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[178431_s_at]

Description T20D3.3 /REP_DB=WormBase Gene ID /WP=CE23989 /TR=O01255 /GB=CAA92486.2 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 45      6grp_high1_10_10 > 54      4grp_highest_8_8 > 09      4grp_high_8_8 > 53      6grp_highest_nonorm > 76      6grp_total > 44      4grp_total > 58      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1260
1 2 3 4 range
A 993.4
(0.2)
P 		1283.3
(2.1)
P 		1423
(3)
P 		515.9
(-3)
P 		907
B 911.3
(-1.7)
P 		1521.1
(3)
P 		1341.7
(1.6)
P 		744.3
(-3)
P 		777
C 1021.4
(-0.6)
P 		1642.1
(2.4)
P 		1775.7
(3)
P 		527.5
(-3)
P 		1248
avr 975.4
(-0.5) 		1482.2
(2.8) 		1513.5
(3) 		595.9
(-3) 		918

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 796
1 2 3 4 5 6 range
A 787.6
(-3)
P 		1087.2
(2.1)
P 		1141.9
(3)
P 		918.2
(-0.8)
P 		836.9
(-2.2)
P 		1107.6
(2.4)
P 		354
B 812.1
(-1.9)
P 		1110.6
(1.4)
P 		1250.9
(3)
P 		890
(-1)
P 		714.8
(-3)
P 		872.9
(-1.2)
P 		536
C 1510.4
(3)
P 		961.2
(-3)
P 		1099.7
(-1.5)
P 		1139.6
(-1.1)
P 		969.1
(-2.9)
P 		1029.1
(-2.3)
P 		549
avr 1036.7
(0.6) 		1053
(0.9) 		1164.2
(3) 		982.6
(-0.4) 		840.3
(-3) 		1003.2
(0) 		324
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 543
1 2 3 4 range
A 361.7
(2.8)
P 		278.6
(1.1)
P 		372
(3)
P 		75.5
(-3)
P 		297
B 290.6
(0.6)
P 		375.7
(3)
P 		310.9
(1.2)
P 		158.6
(-3)
P 		217
C 338.1
(-0.2)
P 		472.1
(1.3)
P 		618.9
(3)
P 		88.9
(-3)
P 		530
avr 330.1
(1.1) 		375.5
(1.9) 		433.9
(3) 		107.7
(-3) 		326

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 415
1 2 3 4 5 6 range
A 145.2
(-3)
P 		386.7
(3)
P 		248.7
(-0.4)
P 		232.9
(-0.8)
P 		252.5
(-0.3)
P 		269.7
(0.1)
P 		242
B 282.6
(-0.9)
P 		369.7
(3)
P 		303.8
(0)
P 		235.7
(-3)
P 		290.4
(-0.6)
P 		325.5
(1)
P 		134
C 559.8
(3)
P 		293.3
(-1.8)
P 		279.9
(-2.1)
P 		229.2
(-3)
P 		313.6
(-1.5)
P 		344.5
(-0.9)
P 		331
avr 329.2
(1.9) 		349.9
(3) 		277.5
(-0.7) 		232.6
(-3) 		285.5
(-0.3) 		313.2
(1.1) 		117
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 178431_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8378
Sequence Type Exemplar sequence
Representative Public ID T20D3.3 NCBI
Target Description T20D3.3 /REP_DB=WormBase Gene ID /WP=CE23989 /TR=O01255 /GB=CAA92486.2 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028220(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028220 NCBI Caenorhabditis elegans T20D3.3a (T20D3.3) mRNA, complete cds. 11/11 None
GENEFINDER00000040172 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:9333568:9335987:-1 11/11 None
T20D3.3.1 ENSEMBL cdna:known chromosome:CEL140:IV:9333319:9335868:-1 gene:T20D3.3 11/11 None
T20D3.3.2 ENSEMBL cdna:known chromosome:CEL140:IV:9333319:9335886:-1 gene:T20D3.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001028221 8/11 Cross Hyb Matching Probes None
SNAP00000040158 4/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
177761 2 1 NM_001028220 178431_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:9333505-9335508(-) 89.55 100.0
Public Domain and Genome References
Ensembl T20D3.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA92486 Hypothetical protein T20D3.3a [Caenorhabditis elegans] ref|NP_001023391.1| T20D3.3a [Caenorhabditis elegans] 1.0E-153
blast CAI79220 Hypothetical protein T20D3.3b [Caenorhabditis elegans] ref|NP_001023392.1| T20D3.3b [Caenorhabditis elegans] 1.0E-129
blast CAA92486 Hypothetical protein T20D3.3a [Caenorhabditis elegans] ref|NP_001023391.1| T20D3.3a [Caenorhabditis elegans] 1.0E-122
blast CAI79220 Hypothetical protein T20D3.3b [Caenorhabditis elegans] ref|NP_001023392.1| T20D3.3b [Caenorhabditis elegans] 2.0E-98
blast CAE74045 Hypothetical protein CBG21696 [Caenorhabditis briggsae] 2.0E-91
Sequence
Target Sequence 
>C. ELEGANS:178431_S_AT
tctcttctccaatgttccatcaaaaaaacatcggagctgtcttcacctgaaacttcaatt
tctctacaatttctttcctactacgaatccattttggtcgattttggtgatcaaattcag
tttctacgaacaaaagcttttcttctctcaaagttacctttcacatcaaactatcagaaa
gaattcaaaaacacgatggcacttctttgtcaattgtgtggatcattcgaaaattggcag
ctttttgaaatcggctcaaaatatttcacgaagttggaaatgtttgggctgaaattgaaa
atgaagaaagttttgaagtatgaaatcgatcattccaaaggatttattcaggagaaattg
aagaagagattggagaaaataacaaaagaattaaatgagttggaaggagaattggaaagt
aatattatcgaatcaattcactcttctatcaactctcacaaagacaccacaacagtcgaa
tccactccaaattcagaatttcgtgctcatgaaagtcgctccaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCTCTTCTCCAATGTTCCATCAAAA 395 613 616 Antisense
AAAAACATCGGAGCTGTCTTCACCT 31 131 639 Antisense
GAGCTGTCTTCACCTGAAACTTCAA 419 387 649 Antisense
ATTTCTTTCCTACTACGAATCCATT 167 17 684 Antisense
GAACAAAAGCTTTTCTTCTCTCAAA 247 353 743 Antisense
GTTACCTTTCACATCAAACTATCAG 629 445 768 Antisense
CACGATGGCACTTCTTTGTCAATTG 341 199 807 Antisense
AATCAATTCACTCTTCTATCAACTC 656 171 1046 Antisense
CTATCAACTCTCACAAAGACACCAC 251 223 1061 Antisense
GACACCACAACAGTCGAATCCACTC 357 367 1078 Antisense
TTCGTGCTCATGAAAGTCGCTCCAA 488 695 1115 Antisense
Sequence Source Affymetrix Proprietary Database