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[178429_at]

Description F21D5.6 /REP_DB=WormBase Gene ID /WP=CE03249 /TR=Q19684 /GB=CAA91036.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 96      6grp_high1_10_10 > 68      4grp_highest_8_8 > 63      4grp_high_8_8 > 27      6grp_highest_nonorm > 87      6grp_total > 59      4grp_total > 36      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 518
1 2 3 4 range
A 1256.3
(1.2)
P 		1297.1
(1.7)
P 		916
(-3)
P 		1403.9
(3)
P 		488
B 1434.1
(3)
P 		1406.1
(2.4)
P 		1137.2
(-3)
P 		1360.4
(1.5)
P 		297
C 1365.8
(1.8)
P 		1409.3
(3)
P 		1189.5
(-3)
P 		1227.3
(-2)
P 		220
avr 1352.1
(2.6) 		1370.8
(3) 		1080.9
(-3) 		1330.5
(2.2) 		290

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 957
1 2 3 4 5 6 range
A 1257.9
(0.7)
P 		995.5
(-1.6)
P 		952.3
(-1.9)
P 		825.7
(-3)
P 		1209.8
(0.3)
P 		1529.9
(3)
P 		704
B 1282.5
(0.1)
P 		1001
(-2.1)
P 		936.5
(-2.6)
P 		887.7
(-3)
P 		1130.5
(-1.1)
P 		1651.9
(3)
P 		764
C 1105
(-1.5)
P 		1038.4
(-1.9)
P 		871.9
(-3)
P 		960.5
(-2.4)
P 		963.7
(-2.4)
P 		1782.8
(3)
P 		911
avr 1215.1
(-0.5) 		1011.6
(-2.1) 		920.2
(-2.8) 		891.3
(-3) 		1101.3
(-1.3) 		1654.9
(3) 		764
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 252
1 2 3 4 range
A 457.4
(3)
P 		281.6
(-1.2)
P 		239.4
(-2.2)
P 		205.5
(-3)
P 		252
B 457.3
(3)
P 		347.3
(-0.4)
P 		263.5
(-3)
P 		289.9
(-2.2)
P 		194
C 452.1
(3)
P 		405.2
(1.9)
P 		414.6
(2.1)
P 		206.9
(-3)
P 		245
avr 455.6
(3) 		344.7
(-0) 		305.8
(-1.1) 		234.1
(-3) 		222

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 423
1 2 3 4 5 6 range
A 231.9
(-2.1)
P 		354.1
(2.3)
P 		207.4
(-3)
P 		209.4
(-2.9)
P 		365.1
(2.7)
P 		372.5
(3)
P 		165
B 446.2
(0.4)
P 		333.2
(-1.4)
P 		227.4
(-3)
P 		235.1
(-2.9)
P 		459.3
(0.6)
P 		616
(3)
P 		389
C 409.6
(0.2)
P 		316.9
(-1.2)
P 		221.9
(-2.6)
P 		193.2
(-3)
P 		311.8
(-1.2)
P 		596.8
(3)
P 		404
avr 362.6
(-0.2) 		334.7
(-0.7) 		218.9
(-2.9) 		212.6
(-3) 		378.7
(0.2) 		528.4
(3) 		316
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178429_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8376
Sequence Type Exemplar sequence
Representative Public ID F21D5.6 NCBI
Target Description F21D5.6 /REP_DB=WormBase Gene ID /WP=CE03249 /TR=Q19684 /GB=CAA91036.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069105(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069105 NCBI Caenorhabditis elegans F21D5.6 (F21D5.6) mRNA, complete cds. 11/11 None
SNAP00000028043 ENSEMBL cdna:SNAP chromosome:CEL140:IV:8740270:8741363:-1 11/11 None
GENEFINDER00000028047 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:8740270:8741363:-1 11/11 None
F21D5.6 ENSEMBL cdna:known chromosome:CEL140:IV:8740150:8741370:-1 gene:F21D5.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:8740275-8741369(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F21D5.6
Entrez Gene 177682 Entrez gene
SwissProt Q19684 EMBL-EBI
WormBase CE03249 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA91036 Hypothetical protein F21D5.6 [Caenorhabditis elegans] ref|NP_501506.1| F21D5.6 [Caenorhabditis elegans] 1.0E-141
blast CAE70020 Hypothetical protein CBG16435 [Caenorhabditis briggsae] 1.0E-100
blast XP_395945 PREDICTED: hypothetical protein XP_395945 [Apis mellifera] 1.0E-16
Sequence
Target Sequence 
>C. ELEGANS:178429_AT
gtcatcgatcattcttctacggtgagagtgtaatcgaaaacggaacaattcacatattca
ctccatacaatctcgtcttcatttgtctcccatatctacaaaagaccgcaggaaaatttg
tggaaattggcgaaatattggtagatgatcaagttgacgggatcaaagaattggctgaaa
atgaaagaattctgaaagcattggaagctgtttcagatgtcaaagatgttctcgatgtca
aactatatcgtttgaacgatcaaaaaatgttggattggatgagaaggaagtttgattctc
tcaaaaaacttctagaaagtgatgcccacaagtctttgttgtcatgtccggaagcattcg
atcgttatgttttcacattcctgtctgaatatctttcttctgatcttgcattgattgtta
aagatcatttgaatattcaagatgctactgtctccgagaacatcgatatgagtatgaaac
gaaaagctgtggatgacgatgagcttgaagataagaagcaacctgtcgcaaagaaaccaa
aagaatcagtacaatcaaagaaacttcaagccgcctcgaagggaaccaaatca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCATCGATCATTCTTCTACGGTGA 689 465 260 Antisense
CATATTCACTCCATACAATCTCGTC 284 215 312 Antisense
CTTCATTTGTCTCCCATATCTACAA 363 221 336 Antisense
GATGCCCACAAGTCTTTGTTGTCAT 374 415 580 Antisense
GTCATGTCCGGAAGCATTCGATCGT 359 467 600 Antisense
GTTATGTTTTCACATTCCTGTCTGA 511 447 623 Antisense
GAATATCTTTCTTCTGATCTTGCAT 148 327 646 Antisense
AAGATGCTACTGTCTCCGAGAACAT 218 155 698 Antisense
GAAGCAACCTGTCGCAAAGAAACCA 411 341 774 Antisense
TCAAAGAAACTTCAAGCCGCCTCGA 222 627 814 Antisense
AGCCGCCTCGAAGGGAACCAAATCA 264 83 828 Antisense
Sequence Source Affymetrix Proprietary Database