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[178427_at]

Description C27A7.7 /REP_DB=WormBase Gene ID /WP=CE08410 /TR=P90760 /GB=CAB02788.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 09      4grp_total > 05      6grp_cgc > 10      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 52
1 2 3 4 range
A 8.6
(-1.9)
A 		2.2
(-3)
A 		4.9
(-2.5)
A 		36.1
(3)
A 		34
B 8.2
(-0.7)
A 		11.8
(2.1)
A 		12.9
(3)
A 		5.2
(-3)
A 		8
C 29.7
(0.1)
A 		2.5
(-3)
A 		3.3
(-2.9)
A 		54.5
(3)
A 		52
avr 15.5
(-0.7) 		5.5
(-3) 		7
(-2.7) 		31.9
(3) 		26

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 37
1 2 3 4 5 6 range
A 38.7
(3)
A 		1.9
(-3)
A 		6.1
(-2.3)
A 		8.1
(-2)
A 		6.7
(-2.2)
A 		12.4
(-1.3)
A 		37
B 1.7
(-3)
A 		6
(-1.9)
A 		24.7
(3)
A 		17.1
(1)
A 		6.8
(-1.7)
A 		3.8
(-2.5)
A 		23
C 4.3
(-1.6)
A 		13.1
(3)
A 		5.3
(-1)
A 		3.6
(-1.9)
A 		5.6
(-0.9)
A 		1.5
(-3)
A 		12
avr 14.9
(3) 		7
(-2.3) 		12
(1.1) 		9.6
(-0.5) 		6.4
(-2.7) 		5.9
(-3) 		9
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 9
1 2 3 4 range
A 3.1
(0.3)
A 		0.5
(-3)
A 		1.3
(-2)
A 		5.3
(3)
A 		5
B 2.6
(1.7)
A 		2.9
(2.7)
A 		3
(3)
A 		1.1
(-3)
A 		2
C 9.8
(3)
A 		0.7
(-3)
A 		1.1
(-2.7)
A 		9.2
(2.6)
A 		9
avr 5.2
(3) 		1.4
(-3) 		1.8
(-2.4) 		5.2
(3) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 7
1 2 3 4 5 6 range
A 7.1
(3)
A 		0.7
(-3)
A 		1.3
(-2.4)
A 		2
(-1.8)
A 		2
(-1.8)
A 		3
(-0.8)
A 		6
B 0.6
(-3)
A 		2
(-1.4)
A 		6
(3)
A 		4.5
(1.3)
A 		2.8
(-0.6)
A 		1.4
(-2.1)
A 		5
C 1.6
(-1.1)
A 		4
(3)
A 		1.4
(-1.5)
A 		0.7
(-2.7)
A 		1.8
(-0.8)
A 		0.5
(-3)
A 		4
avr 3.1
(3) 		2.2
(-0.6) 		2.9
(2.2) 		2.4
(0.2) 		2.2
(-0.6) 		1.6
(-3) 		2
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178427_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13030
Sequence Type Exemplar sequence
Representative Public ID C27A7.7 NCBI
Target Description C27A7.7 /REP_DB=WormBase Gene ID /WP=CE08410 /TR=P90760 /GB=CAB02788.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073650(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073650 NCBI Caenorhabditis elegans Serpentine Receptor, class J family member (srj-4) (srj-4) mRNA, complete cds. 11/11 None
SNAP00000042927 ENSEMBL cdna:SNAP chromosome:CEL140:V:12166398:12167123:1 11/11 None
GENEFINDER00000042933 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:12165971:12167123:1 11/11 None
C27A7.7 ENSEMBL cdna:known chromosome:CEL140:V:12165794:12167123:1 gene:C27A7.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:12165793-12167123(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol srj-4
Ensembl C27A7.7
Entrez Gene 182952 Entrez gene
SwissProt P90760 EMBL-EBI
WormBase CE08410 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB02788 Hypothetical protein C27A7.7 [Caenorhabditis elegans] ref|NP_506051.1| Serpentine Receptor, class J family member (srj-4) [Caenorhabditis elegans] 0.0
blast CAB02788 Hypothetical protein C27A7.7 [Caenorhabditis elegans] ref|NP_506051.1| Serpentine Receptor, class J family member (srj-4) [Caenorhabditis elegans] 1.0E-162
blast CAE75406 Hypothetical protein CBG23396 [Caenorhabditis briggsae] 1.0E-143
blast CAE75405 Hypothetical protein CBG23395 [Caenorhabditis briggsae] 1.0E-141
blast CAE75406 Hypothetical protein CBG23396 [Caenorhabditis briggsae] 1.0E-116
blast CAE75405 Hypothetical protein CBG23395 [Caenorhabditis briggsae] 1.0E-116
blast CAB02788 Hypothetical protein C27A7.7 [Caenorhabditis elegans] ref|NP_506051.1| Serpentine Receptor, class J family member (srj-4) [Caenorhabditis elegans] 1.0E-101
Protein Domains
Database ID Description E-Value
Pfam IPR003002 EMBL-EBI 7TM chemoreceptor, subfamily 1 1.8E-7
Pfam IPR003002 EMBL-EBI 7TM chemoreceptor, subfamily 1 6.0E-8
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_506051.1 7 10-32,45-67,89-111,132-154,200-222,243-265,280-302
C27A7.7 7 10-32,45-67,89-111,132-154,200-222,243-265,280-302
SNAP00000042927 3 46-68,89-111,126-148
GENEFINDER00000042933 5 30-52,73-95,141-163,184-206,221-243
Sequence
Target Sequence 
>C. ELEGANS:178427_AT
cttgttattccctttcgtagagactataatgcagatgttcataaaattccattattggct
agtacctattggggagcatcaacacaactaattctgcgaacttcaactgcgatcattatt
gtaacacttatttcctgcttcacaatatgtttctgcatttggatcggatggacgatacaa
tcaaaattgaaagtagctgacatgagtaaaactactaaaagaatgcacagaaaccttctg
aaagctctggccattcaaactttcattccatttgcaatcagctacatcccatgtgttgtc
gcttggtctgttccaattatccacgtggatactaaaagtttgaacaactatacttctatc
atagctgtagctgcattcccattcatcgatcctttggctatcatggttttcctccctgaa
tatcgaaaattgttcaccaaaatattcctccca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTTGTTATTCCCTTTCGTAGAGACT 622 217 505 Antisense
TCTGCGAACTTCAACTGCGATCATT 567 609 597 Antisense
TTGTAACACTTATTTCCTGCTTCAC 80 709 623 Antisense
AGAAACCTTCTGAAAGCTCTGGCCA 78 73 733 Antisense
AAGCTCTGGCCATTCAAACTTTCAT 135 151 746 Antisense
TTCCATTTGCAATCAGCTACATCCC 635 699 770 Antisense
TACATCCCATGTGTTGTCGCTTGGT 243 645 787 Antisense
TGGTCTGTTCCAATTATCCACGTGG 32 553 808 Antisense
CCCATTCATCGATCCTTTGGCTATC 226 259 882 Antisense
CCTTTGGCTATCATGGTTTTCCTCC 696 265 895 Antisense
ATTGTTCACCAAAATATTCCTCCCA 351 5 933 Antisense
Sequence Source Affymetrix Proprietary Database