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[178425_at]

Description K08D8.6 /REP_DB=WormBase Gene ID /WP=CE11918 /TR=O01636 /GB=CAB05328.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 24      6grp_high1_10_10 > 31      4grp_highest_8_8 > 25      4grp_high_8_8 > 59      6grp_highest_nonorm > 80      6grp_total > 31      4grp_total > 60      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 354
1 2 3 4 range
A 425
(2.1)
P 		350.4
(0.7)
P 		468.5
(3)
P 		165
(-3)
P 		304
B 434.3
(3)
P 		418.3
(2.6)
P 		408.5
(2.4)
P 		167.6
(-3)
P 		267
C 486.8
(2.6)
P 		449.3
(2)
P 		507.7
(3)
P 		153.6
(-3)
P 		354
avr 448.7
(2.7) 		406
(1.9) 		461.6
(3) 		162.1
(-3) 		300

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 180
1 2 3 4 5 6 range
A 153.4
(-2.1)
P 		285.6
(2.3)
P 		307.7
(3)
P 		195.4
(-0.7)
P 		187.3
(-1)
P 		127.6
(-3)
P 		180
B 250.4
(1.5)
P 		234.2
(0.8)
P 		286.1
(3)
P 		207.5
(-0.4)
P 		151.6
(-2.8)
P 		146.8
(-3)
P 		139
C 249
(3)
P 		199.3
(0.4)
P 		227.5
(1.9)
P 		218.3
(1.4)
P 		165.1
(-1.5)
P 		136.1
(-3)
P 		113
avr 217.6
(0.5) 		239.7
(1.5) 		273.8
(3) 		207.1
(0.1) 		168
(-1.6) 		136.8
(-3) 		137
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 153
1 2 3 4 range
A 154.7
(3)
P 		76.1
(-0.6)
P 		122.5
(1.5)
P 		24.2
(-3)
P 		131
B 138.5
(3)
P 		103.3
(0.9)
P 		94.7
(0.4)
P 		35.7
(-3)
P 		103
C 161.1
(2.4)
P 		129.2
(1.1)
P 		177
(3)
P 		25.9
(-3)
P 		151
avr 151.4
(3) 		102.9
(0.6) 		131.4
(2) 		28.6
(-3) 		123

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 73
1 2 3 4 5 6 range
A 28.3
(-3)
P 		101.6
(3)
P 		67
(0.2)
P 		49.6
(-1.3)
P 		56.5
(-0.7)
P 		31.1
(-2.8)
P 		73
B 87.1
(3)
P 		78
(1.3)
P 		69.5
(-0.3)
P 		55
(-3)
P 		61.6
(-1.7)
P 		54.8
(-3)
P 		32
C 92.3
(3)
P 		60.8
(-0.9)
P 		57.9
(-1.3)
P 		43.9
(-3)
P 		53.4
(-1.8)
P 		45.6
(-2.8)
P 		48
avr 69.2
(1.2) 		80.1
(3) 		64.8
(0.5) 		49.5
(-2.1) 		57.2
(-0.8) 		43.8
(-3) 		36
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178425_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8610
Sequence Type Exemplar sequence
Representative Public ID K08D8.6 NCBI
Target Description K08D8.6 /REP_DB=WormBase Gene ID /WP=CE11918 /TR=O01636 /GB=CAB05328.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070056(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070056 NCBI Caenorhabditis elegans K08D8.6 (K08D8.6) mRNA, complete cds. 10/11 None
K08D8.6 ENSEMBL cdna:known chromosome:CEL140:IV:12887110:12890478:-1 gene:K08D8.6 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:12889438-12890476(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl K08D8.6
Entrez Gene 178241 Entrez gene
SwissProt O01636 EMBL-EBI
WormBase CE37842 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA97438 Hypothetical protein K08D8.6 [Caenorhabditis elegans] emb|CAB08161.2| Hypothetical protein K08D8.6 [Caenorhabditis elegans] emb|CAB05328.2| Hypothetical protein K08D8.6 [Caenorhabditis elegans] ref|NP_502457.2| K08D8.6 [Caenorhabditis elegans] 0.0
blast T28080 hypothetical protein ZK896.2 - Caenorhabditis elegans 1.0E-170
blast CAB05320 Hypothetical protein ZK896.5 [Caenorhabditis elegans] ref|NP_502453.1| ZK896.5 [Caenorhabditis elegans] 1.0E-142
Protein Domains
Database ID Description E-Value
Pfam IPR003366 EMBL-EBI Protein of unknown function DUF141 1.2E-43
Sequence
Target Sequence 
>C. ELEGANS:178425_AT
ttaactttatgctctgtattttctagcttgatcgctgacccatataactgtgctggatca
caaattatcaatccacccactaatatcagtaacccttgggtttacccaagtacttggata
aataaaggcaatccacctcagtatgctggggggcaaaattgtacatggactatcaatgtt
ccaaaaggaatgttcgcaatcttcactcttcaaggtattacagatgtgtcatcgcttttg
acaatgcaagattcagtggattacgttactaagttcacaataagtgattcggaaccattt
ttcttgatggatcctttctttgtggtaaatctacaagccaataccaatggaacccttgga
atggaggtcagatggaatccgagtgagtattctttccgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTAACTTTATGCTCTGTATTTTCTA 681 683 43 Antisense
ATTTTCTAGCTTGATCGCTGACCCA 356 15 60 Antisense
ATCGCTGACCCATATAACTGTGCTG 367 37 73 Antisense
ATCACAAATTATCAATCCACCCACT 667 27 99 Antisense
TCCACCCACTAATATCAGTAACCCT 565 587 114 Antisense
AATCCACCTCAGTATGCTGGGGGGC 336 167 172 Antisense
TTCGCAATCTTCACTCTTCAAGGTA 204 697 235 Antisense
GTGATTCGGAACCATTTTTCTTGAT 296 483 326 Antisense
GATGGATCCTTTCTTTGTGGTAAAT 620 407 348 Antisense
ACAAGCCAATACCAATGGAACCCTT 680 111 375 Antisense
GAATCCGAGTGAGTATTCTTTCCGC 697 329 417 Antisense
Sequence Source Affymetrix Proprietary Database