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[178421_at]

Description F13E9.4 /REP_DB=WormBase Gene ID /WP=CE05606 /TR=Q19414 /GB=CAA93412.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 80      4grp_total > 35      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 80
1 2 3 4 range
A 16.5
(-2.1)
A 		76.2
(3)
A 		27.5
(-1.2)
A 		6
(-3)
A 		70
B 40.8
(-0.6)
A 		81.5
(3)
A 		14.5
(-3)
A 		50.4
(0.2)
A 		67
C 1.6
(-3)
A 		64.2
(3)
A 		15.8
(-1.6)
A 		52
(1.8)
A 		63
avr 19.6
(-3) 		74
(3) 		19.3
(-3) 		36.1
(-1.2) 		55

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 59
1 2 3 4 5 6 range
A 14
(-1.9)
A 		8.4
(-2.4)
A 		3.1
(-3)
A 		28.5
(-0.3)
A 		29.9
(-0.2)
A 		60.1
(3)
A 		57
B 19.6
(0.4)
A 		18.7
(0.2)
A 		6.3
(-3)
A 		29.4
(2.9)
A 		29.6
(3)
A 		18.7
(0.2)
A 		23
C 23.5
(-0.1)
A 		38
(1.7)
A 		48.1
(3)
A 		42.1
(2.2)
A 		5.2
(-2.5)
A 		1.1
(-3)
A 		47
avr 19
(-3) 		21.7
(-1.9) 		19.2
(-2.9) 		33.3
(3) 		21.6
(-1.9) 		26.6
(0.2) 		14
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 20
1 2 3 4 range
A 6
(-1)
A 		16.5
(3)
A 		7.2
(-0.6)
A 		0.9
(-3)
A 		16
B 13
(0.4)
A 		20.1
(3)
A 		3.4
(-3)
A 		10.8
(-0.3)
A 		17
C 0.5
(-3)
A 		18.5
(3)
A 		5.5
(-1.3)
A 		8.8
(-0.2)
A 		18
avr 6.5
(-2.5) 		18.4
(3) 		5.4
(-3) 		6.8
(-2.4) 		13

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 14
1 2 3 4 5 6 range
A 2.6
(-2.2)
A 		3
(-2)
A 		0.7
(-3)
A 		7.2
(-0.2)
A 		9
(0.6)
A 		14.6
(3)
A 		14
B 6.8
(0)
A 		6.2
(-0.3)
A 		1.5
(-3)
A 		7.8
(0.6)
A 		12
(3)
A 		7
(0.1)
A 		11
C 8.7
(1.2)
A 		11.6
(2.7)
A 		12.2
(3)
A 		8.5
(1.1)
A 		1.7
(-2.3)
A 		0.4
(-3)
A 		12
avr 6
(-0.6) 		6.9
(1.2) 		4.8
(-3) 		7.8
(3) 		7.6
(2.6) 		7.3
(2) 		3
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178421_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8363
Sequence Type Exemplar sequence
Representative Public ID F13E9.4 NCBI
Target Description F13E9.4 /REP_DB=WormBase Gene ID /WP=CE05606 /TR=Q19414 /GB=CAA93412.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069623(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069623 NCBI Caenorhabditis elegans F13E9.4 (F13E9.4) mRNA, complete cds. 11/11 None
SNAP00000017050 ENSEMBL cdna:SNAP chromosome:CEL140:IV:10895195:10896582:-1 11/11 None
GENEFINDER00000017056 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:10893032:10898628:-1 10/11 None
F13E9.4 ENSEMBL cdna:known chromosome:CEL140:IV:10895195:10896582:-1 gene:F13E9.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:10895200-10896588(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F13E9.4
Entrez Gene 184422 Entrez gene
SwissProt Q19414 EMBL-EBI
WormBase CE05606 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA93412 Hypothetical protein F13E9.4 [Caenorhabditis elegans] ref|NP_502024.1| F13E9.4 [Caenorhabditis elegans] 7.0E-65
blast CAA93412 Hypothetical protein F13E9.4 [Caenorhabditis elegans] ref|NP_502024.1| F13E9.4 [Caenorhabditis elegans] 2.0E-64
blast CAA93411 Hypothetical protein F13E9.9 [Caenorhabditis elegans] ref|NP_502025.2| F13E9.9 [Caenorhabditis elegans] 2.0E-49
blast CAE61951 Hypothetical protein CBG05950 [Caenorhabditis briggsae] 2.0E-42
blast CAE61951 Hypothetical protein CBG05950 [Caenorhabditis briggsae] 5.0E-42
blast CAE61950 Hypothetical protein CBG05949 [Caenorhabditis briggsae] 7.0E-13
Protein Domains
Database ID Description E-Value
Pfam IPR003677 EMBL-EBI Protein of unknown function, DUF 148 7.6E-49
Pfam IPR003677 EMBL-EBI Protein of unknown function, DUF 148 7.6E-49
Pfam IPR003677 EMBL-EBI Protein of unknown function, DUF 148 8.9E-47
Sequence
Target Sequence 
>C. ELEGANS:178421_AT
attcttccagttccaactctgttatgtctaacaatggttattcttccaactcggggtatg
gaaacaacaatggaccaactccttcatttctgaataatgttagttcgtctgctgctcaag
attattacaacattgtaaataacaaatcattgaccaccaaccaaattaacgagcaagcat
ccaattgggcgtcagctaacagtgttcaagcccaatacatccaatacgaaacaaatcgta
gtgctattcacacacaagctacccagaatgtgtctgatattatcaataacttggccacag
ttcaagcatcgattgagggaattcagtcaaacacaagctcatctgaaaatgacaggcaat
ctgcattccaggcattgcaacaagctcatccaatggaatactcggttctaacttcgcttc
aaaaccaacacgagcaaacgcaatatgcaagaatgggtagcaatgggaacagtaatagta
actatggaaactccaattttaattcaaattatggaaacaatggatataaccataatggaa
acaactactttgaaagtacgactgcaacttctgctgca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATTCTTCCAGTTCCAACTCTGTTAT 219 11 629 Antisense
ACAATGGTTATTCTTCCAACTCGGG 25 103 659 Antisense
ACAATGGACCAACTCCTTCATTTCT 691 113 695 Antisense
TAATGTTAGTTCGTCTGCTGCTCAA 63 637 723 Antisense
GCATCCAATTGGGCGTCAGCTAACA 199 309 805 Antisense
ACAGTGTTCAAGCCCAATACATCCA 684 107 827 Antisense
GTGCTATTCACACACAAGCTACCCA 337 479 869 Antisense
GACAGGCAATCTGCATTCCAGGCAT 382 369 979 Antisense
CCAGGCATTGCAACAAGCTCATCCA 75 273 996 Antisense
GAATACTCGGTTCTAACTTCGCTTC 304 173 1024 Antisense
AAGTACGACTGCAACTTCTGCTGCA 166 157 1182 Antisense
Sequence Source Affymetrix Proprietary Database