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[178408_at]

Description B0250.8 /REP_DB=WormBase Gene ID /WP=CE18484 /TR=Q9XVF3 /GB=CAB03796.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 40      4grp_total > 33      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 41
1 2 3 4 range
A 17
(0.1)
A 		24.4
(3)
A 		24.3
(3)
A 		9.3
(-3)
A 		15
B 6.3
(-3)
A 		36.6
(3)
A 		23.3
(0.4)
A 		9
(-2.5)
A 		30
C 6.4
(-3)
A 		6.5
(-3)
A 		7
(-2.9)
A 		47.4
(3)
A 		41
avr 9.9
(-3) 		22.5
(3) 		18.2
(1) 		21.9
(2.7) 		13

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 31
1 2 3 4 5 6 range
A 10.2
(-1.5)
A 		20.9
(0.6)
A 		6.8
(-2.1)
A 		32.8
(3)
A 		2.5
(-3)
A 		5.4
(-2.4)
A 		30
B 5.5
(0.5)
A 		7.5
(2.4)
A 		8.2
(3)
A 		1.7
(-3)
A 		4.9
(-0)
A 		3.2
(-1.6)
A 		7
C 7.1
(2.6)
A 		2.5
(-2.4)
A 		7.5
(3)
A 		2.7
(-2.1)
A 		1.9
(-3)
A 		3.6
(-1.2)
A 		6
avr 7.6
(-0.1) 		10.3
(1.6) 		7.5
(-0.2) 		12.4
(3) 		3.1
(-3) 		4.1
(-2.4) 		9
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 8
1 2 3 4 range
A 6.2
(2.8)
A 		5.3
(1.7)
A 		6.4
(3)
A 		1.4
(-3)
A 		5
B 2
(-2.9)
A 		9
(3)
A 		5.4
(-0)
A 		1.9
(-3)
A 		7
C 2.1
(-2.8)
A 		1.9
(-3)
A 		2.4
(-2.5)
A 		8
(3)
A 		6
avr 3.4
(-3) 		5.4
(3) 		4.7
(0.9) 		3.8
(-1.8) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 8
1 2 3 4 5 6 range
A 1.9
(-2.1)
A 		7.4
(2.3)
A 		1.5
(-2.4)
A 		8.3
(3)
A 		0.7
(-3)
A 		1.3
(-2.5)
A 		8
B 1.9
(1.3)
A 		2.5
(3)
A 		2
(1.6)
A 		0.4
(-3)
A 		2
(1.6)
A 		1.2
(-0.7)
A 		2
C 2.7
(3)
A 		0.8
(-2.4)
A 		1.9
(0.7)
A 		0.6
(-3)
A 		0.6
(-3)
A 		1.2
(-1.3)
A 		2
avr 2.2
(-0.4) 		3.6
(3) 		1.8
(-1.3) 		3.1
(1.8) 		1.1
(-3) 		1.2
(-2.8) 		3
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178408_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13419
Sequence Type Exemplar sequence
Representative Public ID B0250.8 NCBI
Target Description B0250.8 /REP_DB=WormBase Gene ID /WP=CE18484 /TR=Q9XVF3 /GB=CAB03796.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075536(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075536 NCBI Caenorhabditis elegans B0250.8 (B0250.8) mRNA, complete cds. 11/11 None
B0250.8 ENSEMBL cdna:known chromosome:CEL140:V:20467945:20471048:-1 gene:B0250.8 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000043585 6/11 Cross Hyb Matching Probes None
GENEFINDER00000043596 8/11 Cross Hyb Matching Probes None
GENEFINDER00000043599 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:20467944-20471048(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl B0250.8
Entrez Gene 181885 Entrez gene
SwissProt Q9XVF3 EMBL-EBI
WormBase CE18484 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB03796 Hypothetical protein B0250.8 [Caenorhabditis elegans] ref|NP_507937.1| B0250.8 [Caenorhabditis elegans] 1.0E-146
blast NP_491108 LiPid Depleted family member (lpd-6) [Caenorhabditis elegans] gb|AAB97575.2| Lipid depleted protein 6 [Caenorhabditis elegans] 2.0E-10
Sequence
Target Sequence 
>C. ELEGANS:178408_AT
aagcaacctgctactactgaacaagttgaagaagttgaaaatcagaaggagaaggataga
gagattgtgatgaatcgggagagaattttttctttggttcatcgaaacatcacgcgttat
tcgatccaaatgccagcacaaaaaggagtacaagcccttgtatcaaagtttcgtctttct
ccaggtaacccaatgttcctgaaatctagttcagatttattcagcttcacttcttcagac
aaagaatatctggaaggttatgagataaaggtcaaaagcgccgcaggagttatcaaattc
actctcttgaaaggtgatcaaccgcctaaatatgaaatcagttctttccttggaatcatt
ttggaacaccgtgaagatccaagtaatacgccgatgccgccgtcttcgagtcgccgtcat
gcagtggacaatggcaagggagaagaagcgaagtctgtcaccaatgacacagttttcgca
c

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGCAACCTGCTACTACTGAACAAG 362 151 268 Antisense
GAAACATCACGCGTTATTCGATCCA 97 357 371 Antisense
TATTCGATCCAAATGCCAGCACAAA 216 665 385 Antisense
ATCAAAGTTTCGTCTTTCTCCAGGT 552 25 429 Antisense
TTTCTCCAGGTAACCCAATGTTCCT 566 671 443 Antisense
TATTCAGCTTCACTTCTTCAGACAA 688 663 485 Antisense
GCGCCGCAGGAGTTATCAAATTCAC 454 287 545 Antisense
GATCCAAGTAATACGCCGATGCCGC 16 415 643 Antisense
AGTCGCCGTCATGCAGTGGACAATG 149 61 676 Antisense
GAAGAAGCGAAGTCTGTCACCAATG 204 341 709 Antisense
GTCACCAATGACACAGTTTTCGCAC 540 465 724 Antisense
Sequence Source Affymetrix Proprietary Database