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[178391_at]

Description T21E8.5 /REP_DB=WormBase Gene ID /WP=CE06487 /TR=Q22654 /GB=CAA94218.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Cluster 6grp_total > 94      4grp_total > 10      6grp_total_sub > Hat      6grp_rule_based > S5      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 114
1 2 3 4 range
A 22.4
(-2.5)
A 		35.9
(-1.4)
A 		16.6
(-3)
A 		88.1
(3)
A 		72
B 27.5
(-1.4)
A 		14
(-3)
A 		65.8
(3)
A 		57.3
(2)
A 		52
C 21.2
(-2.1)
A 		57.2
(-0.2)
A 		3.4
(-3)
A 		117.3
(3)
A 		114
avr 23.7
(-3) 		35.7
(-1.9) 		28.6
(-2.5) 		87.6
(3) 		64

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 127
1 2 3 4 5 6 range
A 7
(-2.7)
A 		3.4
(-3)
A 		8.1
(-2.6)
A 		82
(3)
A 		65.8
(1.8)
A 		60.1
(1.3)
A 		79
B 14.1
(-2.7)
A 		8.6
(-3)
A 		11.5
(-2.9)
A 		51.4
(-0.9)
A 		130
(3)
P 		46
(-1.2)
A 		121
C 11.3
(-2.9)
A 		49.5
(1.1)
A 		10.8
(-3)
A 		10.4
(-3)
A 		60.1
(2.2)
A 		67.7
(3)
P 		57
avr 10.8
(-2.9) 		20.5
(-2.2) 		10.1
(-3) 		47.9
(0) 		85.3
(3) 		57.9
(0.8) 		75
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 19
1 2 3 4 range
A 8.1
(-0.3)
A 		7.8
(-0.6)
A 		4.3
(-3)
A 		12.9
(3)
A 		9
B 8.8
(-0.3)
A 		3.4
(-3)
A 		15.3
(3)
A 		12.2
(1.4)
A 		12
C 7
(-1.1)
A 		16.5
(1.9)
A 		1.2
(-3)
A 		19.8
(3)
A 		19
avr 8
(-2.2) 		9.2
(-1.3) 		6.9
(-3) 		15
(3) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 52
1 2 3 4 5 6 range
A 1.3
(-3)
A 		1.2
(-3)
A 		1.8
(-2.8)
A 		20.8
(3)
A 		19.9
(2.7)
A 		14.6
(1.1)
A 		20
B 4.9
(-2.7)
A 		2.9
(-3)
A 		2.8
(-3)
A 		13.6
(-1.7)
A 		52.8
(3)
P 		17.1
(-1.3)
A 		50
C 4.2
(-2.4)
A 		15.1
(0.8)
A 		2.7
(-2.8)
A 		2.1
(-3)
A 		19.4
(2)
A 		22.7
(3)
P 		21
avr 3.5
(-2.8) 		6.4
(-2.2) 		2.4
(-3) 		12.2
(-0.9) 		30.7
(3) 		18.1
(0.3) 		28
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178391_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17221
Sequence Type Exemplar sequence
Representative Public ID T21E8.5 NCBI
Target Description T21E8.5 /REP_DB=WormBase Gene ID /WP=CE06487 /TR=Q22654 /GB=CAA94218.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077414(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077414 NCBI Caenorhabditis elegans T21E8.5 (T21E8.5) mRNA, complete cds. 11/11 None
GENEFINDER00000028882 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:10886201:10886975:1 11/11 None
T21E8.5 ENSEMBL cdna:known chromosome:CEL140:X:10886201:10886975:1 gene:T21E8.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:10886200-10886975(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T21E8.5
Entrez Gene 188699 Entrez gene
SwissProt Q22654 EMBL-EBI
WormBase CE06487 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA94218 Hypothetical protein T21E8.5 [Caenorhabditis elegans] ref|NP_509815.1| T21E8.5 [Caenorhabditis elegans] 1.0E-49
blast CAE70804 Hypothetical protein CBG17565 [Caenorhabditis briggsae] 1.0E-23
Sequence
Target Sequence 
>C. ELEGANS:178391_AT
gaaagttgctccactactagaagaagtgatggaccttcgccggaaaattcatattataaa
tgctgaacagtttttaaaaactcgcaacgagcacaccactcttatccttcaagtggaggc
aatgattactgagttctcgcttcacgttttcaaggaacattttgaggcgattcggcgaaa
aggagcgtattgttctgttcgtggagaaagaaactttgtgcgttactcaaagacacttac
tgagttaaacagctcccttcgtcat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAAGTTGCTCCACTACTAGAAGAA 521 359 15 Antisense
GATGGACCTTCGCCGGAAAATTCAT 590 405 42 Antisense
AAAACTCGCAACGAGCACACCACTC 267 131 91 Antisense
CACACCACTCTTATCCTTCAAGTGG 567 195 106 Antisense
GATTACTGAGTTCTCGCTTCACGTT 119 431 138 Antisense
TTCTCGCTTCACGTTTTCAAGGAAC 381 687 148 Antisense
GGAACATTTTGAGGCGATTCGGCGA 338 529 168 Antisense
GGAGCGTATTGTTCTGTTCGTGGAG 660 521 196 Antisense
GAAAGAAACTTTGTGCGTTACTCAA 190 351 220 Antisense
TGCGTTACTCAAAGACACTTACTGA 599 575 233 Antisense
TGAGTTAAACAGCTCCCTTCGTCAT 515 547 255 Antisense
Sequence Source Affymetrix Proprietary Database