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[178390_at]

Description C25G4.3 /REP_DB=WormBase Gene ID /WP=CE05304 /TR=Q18170 /GB=CAA94572.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 56      6grp_high1_10_10 > 75      4grp_highest_8_8 > 16      4grp_high_8_8 > 52      6grp_highest_nonorm > 81      6grp_total > 65      4grp_total > 59      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 191
1 2 3 4 range
A 300.7
(1.3)
P 		354.4
(3)
P 		265.7
(0.2)
P 		163.7
(-3)
P 		191
B 242.9
(0.1)
P 		276.2
(3)
P 		274.2
(2.8)
P 		207.1
(-3)
P 		69
C 274.4
(0.6)
P 		272.8
(0.5)
P 		322.8
(3)
P 		203.4
(-3)
P 		119
avr 272.7
(1.4) 		301.1
(3) 		287.6
(2.3) 		191.4
(-3) 		110

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 163
1 2 3 4 5 6 range
A 210.5
(-2.9)
P 		209.1
(-3)
P 		370.3
(3)
P 		303.4
(0.5)
P 		289.8
(0)
P 		272.9
(-0.6)
P 		161
B 302.5
(-1)
P 		338.4
(1.1)
P 		321.6
(0.1)
P 		282.9
(-2.2)
P 		269.3
(-3)
P 		370.3
(3)
P 		101
C 243.4
(-1.6)
P 		355.7
(2.8)
P 		217.3
(-2.6)
P 		319.6
(1.4)
P 		207.7
(-3)
P 		361.4
(3)
P 		154
avr 252.1
(-3) 		301.1
(0.6) 		303.1
(0.7) 		302
(0.6) 		255.6
(-2.7) 		334.9
(3) 		83
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 89
1 2 3 4 range
A 109.5
(3)
P 		76.9
(0.7)
P 		69.5
(0.2)
P 		24
(-3)
P 		86
B 77.5
(3)
P 		68.2
(1.3)
P 		63.5
(0.5)
P 		44.1
(-3)
P 		33
C 90.8
(1.3)
P 		78.4
(0.4)
P 		112.5
(3)
P 		34.3
(-3)
P 		78
avr 92.6
(3) 		74.5
(1.1) 		81.8
(1.9) 		34.1
(-3) 		59

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 99
1 2 3 4 5 6 range
A 38.8
(-3)
P 		74.4
(1.4)
P 		80.6
(2.1)
P 		77
(1.7)
P 		87.5
(3)
P 		66.4
(0.4)
P 		49
B 105.2
(-0.1)
P 		112.6
(0.6)
P 		78.1
(-2.7)
P 		74.9
(-3)
P 		109.4
(0.3)
P 		138.1
(3)
P 		63
C 90.2
(0.2)
P 		108.5
(1.9)
P 		55.3
(-3)
P 		64.3
(-2.2)
P 		67.2
(-1.9)
P 		121
(3)
P 		66
avr 78.1
(-1.9) 		98.5
(1.4) 		71.3
(-3) 		72.1
(-2.9) 		88
(-0.3) 		108.5
(3) 		37
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178390_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9728
Sequence Type Exemplar sequence
Representative Public ID C25G4.3 NCBI
Target Description C25G4.3 /REP_DB=WormBase Gene ID /WP=CE05304 /TR=Q18170 /GB=CAA94572.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069976(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069976 NCBI Caenorhabditis elegans C25G4.3 (C25G4.3) mRNA, complete cds. 11/11 None
SNAP00000033049 ENSEMBL cdna:SNAP chromosome:CEL140:IV:12444509:12445855:-1 11/11 None
GENEFINDER00000033065 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:12444509:12445855:-1 11/11 None
C25G4.3 ENSEMBL cdna:known chromosome:CEL140:IV:12444421:12445030:-1 gene:C25G4.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:12444514-12445036(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C25G4.3
Entrez Gene 178195 Entrez gene
SwissProt Q18170 EMBL-EBI
WormBase CE05304 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6364 rRNA processing inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA94572 Hypothetical protein C25G4.3 [Caenorhabditis elegans] ref|NP_502377.1| C25G4.3 [Caenorhabditis elegans] 8.0E-93
blast CAA94572 Hypothetical protein C25G4.3 [Caenorhabditis elegans] ref|NP_502377.1| C25G4.3 [Caenorhabditis elegans] 2.0E-92
blast CAE59863 Hypothetical protein CBG03339 [Caenorhabditis briggsae] 9.0E-74
blast CAE59863 Hypothetical protein CBG03339 [Caenorhabditis briggsae] 2.0E-73
Sequence
Target Sequence 
>C. ELEGANS:178390_AT
gatacggtatcgatatgggacttgaagatcgacttctactcgtctctctcggaacaaaac
aacgacgtttagacgacaaaaaagttgttcagctgagcagaatccttgaggaacgaatcg
ccgaagtcgtcgcaacagatgaaatgctcgggcgattacaacttcaaattactcgagtac
gagttgatcgagctttcacacaagtgtccgtttattggatgtgtcgaggtgatggagatt
cggaaattgttgattttctggaagagtcgaaacaccagataagacgacgagtggaggaat
ccattggaattacgtgtcctg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATACGGTATCGATATGGGACTTGA 169 421 23 Antisense
TGAAGATCGACTTCTACTCGTCTCT 280 575 45 Antisense
ACTCGTCTCTCTCGGAACAAAACAA 341 99 60 Antisense
GTTGTTCAGCTGAGCAGAATCCTTG 324 437 106 Antisense
AATCCTTGAGGAACGAATCGCCGAA 670 167 123 Antisense
AATCGCCGAAGTCGTCGCAACAGAT 565 165 138 Antisense
ATGCTCGGGCGATTACAACTTCAAA 118 41 166 Antisense
GATCGAGCTTTCACACAAGTGTCCG 482 415 208 Antisense
AAGTGTCCGTTTATTGGATGTGTCG 482 157 224 Antisense
GGATGTGTCGAGGTGATGGAGATTC 239 515 239 Antisense
GAATCCATTGGAATTACGTGTCCTG 19 325 319 Antisense
Sequence Source Affymetrix Proprietary Database