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[178363_at]

Description T21E8.4 /REP_DB=WormBase Gene ID /WP=CE06486 /TR=Q22653 /GB=CAA94217.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Cluster 6grp_total > 72      4grp_total > 58      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 39
1 2 3 4 range
A 2.6
(-3)
A 		2.8
(-3)
A 		40.1
(3)
A 		11.1
(-1.6)
A 		38
B 22
(1)
A 		29.2
(3)
A 		24.4
(1.7)
A 		7.7
(-3)
A 		22
C 30.5
(-0.2)
A 		42
(3)
A 		21.3
(-2.8)
A 		20.5
(-3)
A 		22
avr 18.4
(-0.9) 		24.7
(1.5) 		28.6
(3) 		13.1
(-3) 		16

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 37
1 2 3 4 5 6 range
A 31.5
(1.8)
A 		3.7
(-3)
A 		36.5
(2.7)
A 		38.4
(3)
A 		38.5
(3)
A 		16
(-0.9)
A 		35
B 5.5
(-2)
A 		5.9
(-1.9)
A 		24.9
(3)
A 		1.8
(-3)
A 		18.1
(1.2)
A 		16.9
(0.9)
A 		23
C 2.8
(-3)
A 		28.9
(3)
A 		3.3
(-2.9)
A 		19.3
(0.8)
A 		4.6
(-2.6)
A 		2.7
(-3)
A 		26
avr 13.3
(-2.1) 		12.8
(-2.4) 		21.6
(3) 		19.8
(1.9) 		20.4
(2.3) 		11.9
(-3) 		10
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 12
1 2 3 4 range
A 1
(-2.8)
A 		0.6
(-3)
A 		10.5
(3)
A 		1.6
(-2.4)
A 		10
B 7
(2.8)
A 		7.2
(3)
A 		5.7
(1.4)
A 		1.6
(-3)
A 		6
C 10.1
(1.6)
A 		12.1
(3)
A 		7.4
(-0.3)
A 		3.5
(-3)
A 		9
avr 6
(1) 		6.6
(1.6) 		7.9
(3) 		2.2
(-3) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 11
1 2 3 4 5 6 range
A 5.8
(-0.4)
A 		1.3
(-3)
A 		7.9
(0.8)
A 		9.7
(1.9)
A 		11.6
(3)
A 		3.9
(-1.5)
A 		10
B 1.9
(-1.8)
A 		2
(-1.7)
A 		6.1
(1.9)
A 		0.5
(-3)
A 		7.3
(3)
A 		6.3
(2.1)
A 		7
C 1
(-2.9)
A 		8.8
(3)
A 		0.8
(-3)
A 		3.9
(-0.7)
A 		1.5
(-2.5)
A 		0.9
(-2.9)
A 		8
avr 2.9
(-3) 		4
(-1.3) 		4.9
(0.1) 		4.7
(-0.2) 		6.8
(3) 		3.7
(-1.8) 		4
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178363_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17089
Sequence Type Exemplar sequence
Representative Public ID T21E8.4 NCBI
Target Description T21E8.4 /REP_DB=WormBase Gene ID /WP=CE06486 /TR=Q22653 /GB=CAA94217.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077413(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077413 NCBI Caenorhabditis elegans T21E8.4 (T21E8.4) mRNA, complete cds. 11/11 None
GENEFINDER00000028877 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:10882207:10884431:1 11/11 None
SNAP00000028876 ENSEMBL cdna:SNAP chromosome:CEL140:X:10883490:10884431:1 10/11 None
T21E8.4 ENSEMBL cdna:known chromosome:CEL140:X:10880765:10884431:1 gene:T21E8.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000028875 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:10880764-10884431(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T21E8.4
Entrez Gene 188698 Entrez gene
SwissProt Q22653 EMBL-EBI
WormBase CE06486 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA94217 Hypothetical protein T21E8.4 [Caenorhabditis elegans] ref|NP_509814.1| T21E8.4 [Caenorhabditis elegans] 0.0
blast CAA94217 Hypothetical protein T21E8.4 [Caenorhabditis elegans] ref|NP_509814.1| T21E8.4 [Caenorhabditis elegans] 1.0E-128
blast CAA94217 Hypothetical protein T21E8.4 [Caenorhabditis elegans] ref|NP_509814.1| T21E8.4 [Caenorhabditis elegans] 5.0E-95
blast CAE70805 Hypothetical protein CBG17566 [Caenorhabditis briggsae] 5.0E-32
blast CAE70805 Hypothetical protein CBG17566 [Caenorhabditis briggsae] 6.0E-28
blast CAE70805 Hypothetical protein CBG17566 [Caenorhabditis briggsae] 7.0E-28
blast CAE70806 Hypothetical protein CBG17567 [Caenorhabditis briggsae] 6.0E-18
Sequence
Target Sequence 
>C. ELEGANS:178363_AT
gatcaggtgcctttatcgtcatgcacgcaagcgagaactatgataacttggaccaggtgt
ttttggagcagtggggaacgttcaatgaggcgagtcacttcatcagatttgcgctggatc
acgaaattatggaaaactggcagcacatcattgaacttctggaaacttgcgaagctcgaa
tggaaatcatcactgacttcgattacatgcgtgcatcaagctatgaagtgatgagaattg
tcaatacgcgtatgactgacgtcctcaacgtcatccttccagtcaaagaattcatggagg
tctccatcagactctacggagaaagaatggtggatgaagcagaagaaactagaaagatga
acgcattggagcaggaagctgctggatcttcgaaagcgcagaaactcacgttttcagaaa
atgatgtagttgaagcttctaacagtaaggcaaacgaagaccgtgtcaaacgcaaagagc
tcattgatgccattaaagaagccgaggaaactgatctccttcttaatcaacgtcgcgaaa
aacggaatgacaacgaagaaccaccacgaagac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATCAGGTGCCTTTATCGTCATGCA 378 421 578 Antisense
AGGCGAGTCACTTCATCAGATTTGC 583 53 665 Antisense
ATCAGATTTGCGCTGGATCACGAAA 301 27 679 Antisense
ACTGACTTCGATTACATGCGTGCAT 570 97 769 Antisense
TACGCGTATGACTGACGTCCTCAAC 214 649 822 Antisense
AACGTCATCCTTCCAGTCAAAGAAT 598 143 844 Antisense
GAGGTCTCCATCAGACTCTACGGAG 278 403 874 Antisense
AAGCTGCTGGATCTTCGAAAGCGCA 647 149 953 Antisense
GAAAGCGCAGAAACTCACGTTTTCA 478 359 969 Antisense
TCTCCTTCTTAATCAACGTCGCGAA 672 609 1092 Antisense
GACAACGAAGAACCACCACGAAGAC 572 361 1126 Antisense
Sequence Source Affymetrix Proprietary Database