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[178347_at]

Description F33H2.8 /REP_DB=WormBase Gene ID /WP=CE17769 /TR=O62221 /GB=CAB04266.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 17      6grp_high1_10_10 > 60      6grp_highest_nonorm > 71      6grp_total > 61      4grp_total > 49      6grp_highest_sub > 04      6grp_high1_sub > 04      6grp_total_sub > Hat      6grp_rule_based > S4      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 426
1 2 3 4 range
A 40.6
(-3)
A 		443.6
(3)
P 		365.5
(1.8)
P 		93.3
(-2.2)
A 		403
B 80.4
(-2.5)
A 		466.7
(3)
P 		432.7
(2.5)
P 		48.1
(-3)
A 		419
C 49.5
(-3)
A 		383
(2.4)
P 		420.9
(3)
P 		47.5
(-3)
A 		373
avr 56.8
(-3) 		431.1
(3) 		406.4
(2.6) 		63
(-2.9) 		374

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 721
1 2 3 4 5 6 range
A 365.1
(-0.2)
P 		261.6
(-1.4)
P 		617.5
(2.7)
P 		645.5
(3)
P 		324.4
(-0.7)
P 		121.1
(-3)
P 		524
B 275.8
(-1.6)
P 		338.8
(-1.1)
P 		417.6
(-0.4)
P 		821.9
(3)
P 		284.3
(-1.5)
P 		111.3
(-3)
P 		711
C 289.7
(-0.7)
P 		286.8
(-0.8)
P 		463.3
(1.3)
P 		603.4
(3)
P 		277.5
(-0.9)
P 		101
(-3)
P 		502
avr 310.2
(-0.9) 		295.7
(-1.1) 		499.5
(1) 		690.3
(3) 		295.4
(-1.1) 		111.1
(-3) 		579
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 139
1 2 3 4 range
A 14.8
(-2.9)
A 		96.3
(3)
P 		95.6
(2.9)
P 		13.7
(-3)
A 		83
B 25.6
(-2.1)
A 		115.3
(3)
P 		100.3
(2.1)
P 		10.3
(-3)
A 		105
C 16.4
(-2.6)
A 		110.1
(1.4)
P 		146.7
(3)
P 		8
(-3)
A 		139
avr 18.9
(-2.5) 		107.2
(2.6) 		114.2
(3) 		10.7
(-3) 		104

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 188
1 2 3 4 5 6 range
A 67.3
(-1.3)
P 		93
(-0.2)
P 		134.5
(1.7)
P 		163.7
(3)
P 		97.9
(0.1)
P 		29.5
(-3)
P 		134
B 95.9
(-1.1)
P 		112.8
(-0.6)
P 		101.4
(-1)
P 		217.7
(3)
P 		115.5
(-0.5)
P 		41.5
(-3)
P 		176
C 107.4
(2)
P 		87.5
(0.7)
P 		117.9
(2.8)
P 		121.3
(3)
P 		89.8
(0.8)
P 		33.8
(-3)
P 		88
avr 90.2
(-0.5) 		97.8
(-0.2) 		117.9
(0.8) 		167.6
(3) 		101.1
(-0) 		34.9
(-3) 		133
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178347_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.693
Sequence Type Exemplar sequence
Representative Public ID F33H2.8 NCBI
Target Description F33H2.8 /REP_DB=WormBase Gene ID /WP=CE17769 /TR=O62221 /GB=CAB04266.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061220(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061220 NCBI Caenorhabditis elegans F33H2.8 (F33H2.8) mRNA, complete cds. 11/11 None
GENEFINDER00000009490 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:15035890:15041079:-1 11/11 None
SNAP00000009481 ENSEMBL cdna:SNAP chromosome:CEL140:I:15039805:15041079:-1 11/11 None
F33H2.8 ENSEMBL cdna:known chromosome:CEL140:I:15039295:15041157:-1 gene:F33H2.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:15039735-15041088(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F33H2.8
Entrez Gene 185238 Entrez gene
SwissProt O62221 EMBL-EBI
WormBase CE37514 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04267 Hypothetical protein F33H2.7 [Caenorhabditis elegans] ref|NP_493620.2| F33H2.7 [Caenorhabditis elegans] 0.0
blast CAB04266 Hypothetical protein F33H2.8 [Caenorhabditis elegans] ref|NP_493621.2| F33H2.8 [Caenorhabditis elegans] 1.0E-176
blast CAE72548 Hypothetical protein CBG19732 [Caenorhabditis briggsae] 1.0E-148
blast CAB04266 Hypothetical protein F33H2.8 [Caenorhabditis elegans] ref|NP_493621.2| F33H2.8 [Caenorhabditis elegans] 1.0E-139
blast CAB04266 Hypothetical protein F33H2.8 [Caenorhabditis elegans] ref|NP_493621.2| F33H2.8 [Caenorhabditis elegans] 1.0E-116
blast CAE72549 Hypothetical protein CBG19733 [Caenorhabditis briggsae] 2.0E-88
blast CAE72549 Hypothetical protein CBG19733 [Caenorhabditis briggsae] 1.0E-87
Protein Domains
Database ID Description E-Value
Pfam IPR002893 EMBL-EBI Zn-finger, MYND type 6.8E-7
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_493621.2 8 42-64,69-91,128-146,161-175,182-204,219-241,253-275,305-327
F33H2.8 8 42-64,69-91,128-146,161-175,182-204,219-241,253-275,305-327
GENEFINDER00000009490 7 13-35,45-67,102-120,135-149,156-178,193-215,227-249
SNAP00000009481 7 13-35,40-62,75-92,102-121,134-151,156-178,200-222
Sequence
Target Sequence 
>C. ELEGANS:178347_AT
gctctcggcggctcataatgtactattttttgtattggacattaagctcatcgccacaag
cttcgcccagtttttcttcaccttcatttttctgaaactgatccattttgcaaattatat
tccacaggcgcatattcccaagaagatacttctcccggcagccggtgccaaactactcga
ggttctgattgcctctcttgctaactcacacaacagaaccggagagctgtttcttgtcag
aagtttcgatttcctatttgccggatgcattctcttctactactcgacgcgtcaaaatgg
gaagggcttgactaaaacggactgttggatgattgctccccttgcgatcgcagtgtcaat
agcctggcttgaacctggacaaatggaatatacgggagtttctttgatgtccgcatttct
gctcccaatttcaagagccctctcgttcattttactaaaaaactcgatggattgtttagg
aaaaggtcatatcaatgcttttatgctggaatacactcgatttgttactattctactctt
cct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCTCTCGGCGGCTCATAATGTACTA 646 297 168 Antisense
TATATTCCACAGGCGCATATTCCCA 523 661 283 Antisense
GGTGCCAAACTACTCGAGGTTCTGA 66 503 331 Antisense
GAAGTTTCGATTTCCTATTTGCCGG 50 337 407 Antisense
TATTTGCCGGATGCATTCTCTTCTA 644 661 422 Antisense
TCTTCTACTACTCGACGCGTCAAAA 337 617 440 Antisense
CGGACTGTTGGATGATTGCTCCCCT 335 243 485 Antisense
AATAGCCTGGCTTGAACCTGGACAA 57 173 525 Antisense
TTTCTTTGATGTCCGCATTTCTGCT 538 671 566 Antisense
TTCAAGAGCCCTCTCGTTCATTTTA 590 683 597 Antisense
GATTTGTTACTATTCTACTCTTCCT 479 427 686 Antisense
Sequence Source Affymetrix Proprietary Database