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[178337_at]

Description F28G4.4 /REP_DB=WormBase Gene ID /WP=CE15922 /TR=O17854 /GB=CAB07607.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 81      4grp_total > 49      6grp_total_sub > Hat      6grp_rule_based > S4      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 108
1 2 3 4 range
A 6.7
(-3)
A 		65.5
(0.3)
A 		113.8
(3)
A 		11.9
(-2.7)
A 		107
B 35.8
(-1.2)
A 		106.6
(3)
A 		102.6
(2.8)
A 		5.4
(-3)
A 		101
C 15.7
(-3)
A 		106.9
(3)
P 		83.7
(1.5)
P 		56.6
(-0.3)
A 		91
avr 19.4
(-3) 		93
(2.5) 		100
(3) 		24.6
(-2.6) 		81

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 148
1 2 3 4 5 6 range
A 58.5
(-1.1)
A 		63.5
(-0.9)
A 		41.2
(-1.9)
A 		152.8
(3)
P 		86
(0.1)
A 		16.2
(-3)
A 		137
B 20.3
(-2.7)
A 		35.9
(-2)
A 		74.3
(-0.1)
A 		132.1
(2.6)
P 		140.1
(3)
P 		14.1
(-3)
A 		126
C 18
(-2.5)
A 		44.7
(-1.5)
A 		78.7
(-0.1)
A 		154.6
(3)
P 		106.9
(1.1)
P 		6.5
(-3)
A 		148
avr 32.3
(-2.1) 		48
(-1.4) 		64.7
(-0.7) 		146.5
(3) 		111
(1.4) 		12.3
(-3) 		134
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 30
1 2 3 4 range
A 2.4
(-2.9)
A 		14.2
(-0.3)
A 		29.8
(3)
A 		1.7
(-3)
A 		28
B 11.4
(-0.5)
A 		26.3
(3)
A 		23.8
(2.4)
A 		1.1
(-3)
A 		25
C 5.2
(-3)
A 		30.7
(3)
P 		29.2
(2.6)
P 		9.5
(-2)
A 		26
avr 6.3
(-2.4) 		23.7
(2) 		27.6
(3) 		4.1
(-3) 		24

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 55
1 2 3 4 5 6 range
A 10.8
(-1.8)
A 		22.6
(0.2)
A 		9
(-2.1)
A 		38.8
(3)
P 		26
(0.8)
A 		3.9
(-3)
A 		35
B 7.1
(-2.8)
A 		12
(-2.2)
A 		18
(-1.5)
A 		35
(0.5)
P 		56.9
(3)
P 		5.2
(-3)
A 		52
C 6.7
(-2.2)
A 		13.6
(-0.9)
A 		20
(0.3)
A 		31.1
(2.4)
P 		34.6
(3)
P 		2.2
(-3)
A 		32
avr 8.2
(-2.3) 		16.1
(-0.9) 		15.7
(-1) 		35
(2.3) 		39.2
(3) 		3.8
(-3) 		35
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178337_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12786
Sequence Type Exemplar sequence
Representative Public ID F28G4.4 NCBI
Target Description F28G4.4 /REP_DB=WormBase Gene ID /WP=CE15922 /TR=O17854 /GB=CAB07607.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074710(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074710 NCBI Caenorhabditis elegans F28G4.4 (F28G4.4) mRNA, complete cds. 11/11 None
SNAP00000032994 ENSEMBL cdna:SNAP chromosome:CEL140:V:16283219:16288275:-1 9/11 None
GENEFINDER00000033000 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:16283219:16288275:-1 10/11 None
F28G4.4 ENSEMBL cdna:known chromosome:CEL140:V:16285601:16288275:-1 gene:F28G4.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:16285600-16288275(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F28G4.4
Entrez Gene 185082 Entrez gene
SwissProt O17854 EMBL-EBI
WormBase CE35511 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB07607 Hypothetical protein F28G4.4 [Caenorhabditis elegans] ref|NP_507111.2| F28G4.4 [Caenorhabditis elegans] 0.0
blast CAB07605 Hypothetical protein F28G4.2 [Caenorhabditis elegans] ref|NP_507110.1| F28G4.2 [Caenorhabditis elegans] 0.0
blast CAB07607 Hypothetical protein F28G4.4 [Caenorhabditis elegans] ref|NP_507111.2| F28G4.4 [Caenorhabditis elegans] 1.0E-168
blast CAE56603 Hypothetical protein CBG24357 [Caenorhabditis briggsae] 1.0E-153
blast CAE56639 Hypothetical protein CBG24399 [Caenorhabditis briggsae] 1.0E-148
Protein Domains
Database ID Description E-Value
Pfam IPR005069 EMBL-EBI Protein of unknown function DUF271 1.0E-126
Pfam IPR005069 EMBL-EBI Protein of unknown function DUF271 1.0E-126
Pfam IPR005069 EMBL-EBI Protein of unknown function DUF271 1.0E-126
Pfam IPR005069 EMBL-EBI Protein of unknown function DUF271 1.0E-126
Pfam IPR005069 EMBL-EBI Protein of unknown function DUF271 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_507111.2 1 5-27
F28G4.4 1 5-27
SNAP00000032994 1 5-27
GENEFINDER00000033000 1 5-27
Sequence
Target Sequence 
>C. ELEGANS:178337_AT
agactgggtatgcggtgcgacgtttttcgtaagaggcaacccgacaacgttccagttttt
cagtcaggtcgaatccttcatgttatcccatcaaagcccagattccacaattttcaccta
tttatgtggccatcatcattacaaatgtcaatttctaccccaatggatggtttcctcatt
caactatttcgaaggaccccgggaaaatgtacctgttcttattcaaattgacggtggaaa
acaaaaaggggagtctaaaatggatgtattgagaaggggcaattttgtgtttcaacatga
aaatggcacttgtgatgagacgaattttcataaattgaggcaagaagttttgaaaaatat
tcttattaaacaaatttttttcagggaaaacgtgaagtttggcttcccagatttcatccg
aaaagatggctcactgaaagaaaattcgttccagaaaactgtatattttatgaaaaacct
gttcaatgtggacccatgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGACTGGGTATGCGGTGCGACGTTT 84 67 825 Antisense
ACAACGTTCCAGTTTTTCAGTCAGG 91 107 868 Antisense
CAGTCAGGTCGAATCCTTCATGTTA 260 207 885 Antisense
TTATCCCATCAAAGCCCAGATTCCA 412 679 907 Antisense
CCAGATTCCACAATTTTCACCTATT 160 271 922 Antisense
TTCACCTATTTATGTGGCCATCATC 389 687 937 Antisense
AATGTCAATTTCTACCCCAATGGAT 649 163 968 Antisense
GGATGGTTTCCTCATTCAACTATTT 248 515 989 Antisense
GGGAAAATGTACCTGTTCTTATTCA 81 493 1025 Antisense
AAGTTTGGCTTCCCAGATTTCATCC 371 157 1219 Antisense
AAACCTGTTCAATGTGGACCCATGG 395 123 1299 Antisense
Sequence Source Affymetrix Proprietary Database