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[178334_at]

Description F08G5.3 /REP_DB=WormBase Gene ID /WP=CE05573 /TR=Q19219 /GB=CAA94582.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 82      6grp_high1_10_10 > 38      4grp_highest_8_8 > 31      4grp_high_8_8 > 12      6grp_highest_nonorm > 80      6grp_total > 37      4grp_total > 12      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 480
1 2 3 4 range
A 278.6
(-1)
P 		156.3
(-3)
P 		167.6
(-2.8)
P 		520.7
(3)
P 		364
B 262.3
(-2.1)
P 		253.7
(-2.3)
P 		199.4
(-3)
P 		636.4
(3)
P 		437
C 296.9
(-0.4)
P 		316
(0.1)
P 		208.2
(-3)
P 		413.7
(3)
P 		206
avr 279.3
(-1.4) 		242
(-2.1) 		191.7
(-3) 		523.6
(3) 		332

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 205
1 2 3 4 5 6 range
A 249.1
(3)
P 		207.4
(0.8)
P 		183.7
(-0.5)
P 		136.9
(-3)
P 		193.6
(0)
P 		180.8
(-0.7)
P 		112
B 329.9
(3)
P 		213.9
(-1.1)
P 		198.9
(-1.7)
P 		195.2
(-1.8)
P 		161.7
(-3)
P 		210.9
(-1.2)
P 		168
C 259.8
(1.7)
P 		227
(0.5)
P 		124.7
(-3)
P 		164.8
(-1.6)
P 		148.5
(-2.2)
P 		297.7
(3)
P 		173
avr 279.6
(3) 		216.1
(-0.3) 		169.1
(-2.8) 		165.6
(-3) 		167.9
(-2.9) 		229.8
(0.4) 		114
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 102
1 2 3 4 range
A 101.4
(3)
P 		33.9
(-3)
P 		43.8
(-2.1)
P 		76.2
(0.8)
P 		68
B 83.7
(-0.5)
P 		62.6
(-1.9)
P 		46.2
(-3)
P 		135.6
(3)
P 		89
C 98.3
(3)
P 		90.9
(1.4)
P 		72.6
(-2.4)
P 		69.8
(-3)
P 		29
avr 94.5
(3) 		62.5
(-1.8) 		54.2
(-3) 		93.9
(2.9) 		40

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 83
1 2 3 4 5 6 range
A 45.9
(-1.3)
P 		73.8
(3)
P 		40
(-2.2)
P 		34.7
(-3)
P 		58.4
(0.6)
P 		44
(-1.6)
P 		39
B 114.8
(3)
P 		71.2
(-0.9)
P 		48.3
(-3)
P 		51.7
(-2.7)
P 		65.7
(-1.4)
P 		78.7
(-0.3)
P 		67
C 96.3
(2.7)
P 		69.3
(0.3)
P 		31.7
(-3)
P 		33.1
(-2.9)
P 		48.1
(-1.6)
P 		99.6
(3)
P 		68
avr 85.7
(3) 		71.4
(1.1) 		40
(-3) 		39.8
(-3) 		57.4
(-0.7) 		74.1
(1.5) 		46
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178334_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8577
Sequence Type Exemplar sequence
Representative Public ID F08G5.3 NCBI
Target Description F08G5.3 /REP_DB=WormBase Gene ID /WP=CE05573 /TR=Q19219 /GB=CAA94582.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027950(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027950 NCBI Caenorhabditis elegans F08G5.3b (F08G5.3) mRNA, complete cds. 10/11 None
SNAP00000043804 ENSEMBL cdna:SNAP chromosome:CEL140:IV:12408082:12414683:-1 11/11 None
GENEFINDER00000043823 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:12413058:12414683:-1 11/11 None
F08G5.3b ENSEMBL cdna:known chromosome:CEL140:IV:12412945:12414683:-1 gene:F08G5.3 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001027949 5/11 Cross Hyb Matching Probes None
F08G5.3a.2 5/11 Cross Hyb Matching Probes None
F08G5.3a.1 5/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
184204 2 1 NM_001027950 178334_at (10)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:12413063-12414689(-) 100.0 100.0
Public Domain and Genome References
Ensembl F08G5.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAE46662 Hypothetical protein F08G5.3b [Caenorhabditis elegans] ref|NP_001023121.1| F08G5.3b [Caenorhabditis elegans] 1.0E-92
blast CAE46662 Hypothetical protein F08G5.3b [Caenorhabditis elegans] ref|NP_001023121.1| F08G5.3b [Caenorhabditis elegans] 2.0E-76
blast CAE59858 Hypothetical protein CBG03332 [Caenorhabditis briggsae] 2.0E-76
blast CAE46662 Hypothetical protein F08G5.3b [Caenorhabditis elegans] ref|NP_001023121.1| F08G5.3b [Caenorhabditis elegans] 9.0E-76
blast CAE59858 Hypothetical protein CBG03332 [Caenorhabditis briggsae] 9.0E-76
blast CAE59858 Hypothetical protein CBG03332 [Caenorhabditis briggsae] 9.0E-73
blast CAA94582 Hypothetical protein F08G5.3a [Caenorhabditis elegans] ref|NP_001023120.1| F08G5.3a [Caenorhabditis elegans] 8.0E-69
Sequence
Target Sequence 
>C. ELEGANS:178334_AT
atgaagtcgtcaagtatgccccaactgaagacaacgaaattcatgcattgatacgtggca
aaccgtcattctgtaaagctaatattcgagttaatacacagcagttccaagagagactta
cctaccttgacaaacgatatgatcatcttcggaaaatgacacattcactgagaaagaaag
ttaatgaacttgaggacataatgcgattggacaatgacgaggagaacatggataccattc
aaaagttattggacgaaatcaaacgagaaaaacaattgatgcgtgacgaggcacatataa
tcaaaggagaactatcaaaaaccatgtataatgaggatttaagacgtagattggctggcg
aatggaagaaaattgacgcagacagaaaaagctctgcggccgaatccgaagcttcttcgt
caatgattggtg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGAAGTCGTCAAGTATGCCCCAAC 242 43 26 Antisense
GTATGCCCCAACTGAAGACAACGAA 304 451 39 Antisense
GATACGTGGCAAACCGTCATTCTGT 692 423 75 Antisense
GGCAAACCGTCATTCTGTAAAGCTA 469 535 82 Antisense
GAGTTAATACACAGCAGTTCCAAGA 87 397 113 Antisense
AGAGAGACTTACCTACCTTGACAAA 365 69 135 Antisense
AATTGATGCGTGACGAGGCACATAT 391 175 299 Antisense
AAGACGTAGATTGGCTGGCGAATGG 498 151 366 Antisense
CAGAAAAAGCTCTGCGGCCGAATCC 304 205 408 Antisense
GGCCGAATCCGAAGCTTCTTCGTCA 707 537 423 Antisense
GAAGCTTCTTCGTCAATGATTGGTG 138 343 433 Antisense
Sequence Source Affymetrix Proprietary Database