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[178331_at]

Description K09E4.4 /REP_DB=WormBase Gene ID /WP=CE19997 /TR=Q9NAP6 /GB=CAB70170.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 50      6grp_high1_10_10 > 02      4grp_high_8_8 > 12      6grp_highest_nonorm > 90      6grp_total > 24      4grp_total > 13      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 493
1 2 3 4 range
A 303.3
(-0.2)
P 		145.1
(-2.1)
P 		69.9
(-3)
A 		563
(3)
P 		493
B 235.2
(-0.9)
P 		134.7
(-2.6)
P 		112
(-3)
P 		468.6
(3)
P 		357
C 296
(0.6)
P 		150.1
(-2)
P 		91.5
(-3)
P 		429.1
(3)
P 		338
avr 278.2
(-0.2) 		143.3
(-2.2) 		91.1
(-3) 		486.9
(3) 		396

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 123
1 2 3 4 5 6 range
A 221.1
(3)
P 		172.4
(-0.3)
P 		195.3
(1.2)
P 		151.2
(-1.8)
P 		133.3
(-3)
P 		147.5
(-2)
P 		88
B 236.2
(2.4)
P 		197
(-0.1)
P 		245.2
(3)
P 		163.5
(-2.2)
P 		150.4
(-3)
P 		165.8
(-2)
P 		95
C 233
(3)
P 		222.5
(2.4)
P 		122.3
(-3)
P 		163.2
(-0.8)
P 		136.1
(-2.3)
P 		161
(-0.9)
P 		111
avr 230.1
(3) 		197.3
(0.8) 		187.6
(0.2) 		159.3
(-1.7) 		139.9
(-3) 		158.1
(-1.8) 		90
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 92
1 2 3 4 range
A 110.4
(3)
P 		31.5
(-2.1)
P 		18.3
(-3)
A 		82.4
(1.2)
P 		92
B 75
(1)
P 		33.3
(-2.4)
P 		26
(-3)
P 		99.9
(3)
P 		74
C 98
(3)
P 		43.2
(-2)
P 		31.9
(-3)
P 		72.3
(0.7)
P 		66
avr 94.5
(3) 		36
(-2.1) 		25.4
(-3) 		84.9
(2.2) 		69

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 55
1 2 3 4 5 6 range
A 40.8
(-1.8)
P 		61.3
(3)
P 		42.5
(-1.4)
P 		38.3
(-2.4)
P 		40.2
(-2)
P 		35.9
(-3)
P 		25
B 82.2
(3)
P 		65.6
(0.4)
P 		59.5
(-0.5)
P 		43.3
(-3)
P 		61.1
(-0.3)
P 		61.8
(-0.1)
P 		39
C 86.4
(3)
P 		67.9
(1)
P 		31.1
(-3)
P 		32.8
(-2.8)
P 		44.1
(-1.6)
P 		53.9
(-0.5)
P 		55
avr 69.8
(3) 		64.9
(2.1) 		44.4
(-1.8) 		38.1
(-3) 		48.5
(-1) 		50.5
(-0.7) 		32
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178331_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3878
Sequence Type Exemplar sequence
Representative Public ID K09E4.4 NCBI
Target Description K09E4.4 /REP_DB=WormBase Gene ID /WP=CE19997 /TR=Q9NAP6 /GB=CAB70170.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064547(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064547 NCBI Caenorhabditis elegans K09E4.4 (K09E4.4) mRNA, complete cds. 11/11 A 
SNAP00000022863 ENSEMBL cdna:SNAP chromosome:CEL140:II:14114190:14123808:-1 11/11 None
GENEFINDER00000022873 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:14114190:14117996:-1 11/11 None
K09E4.4.2 ENSEMBL cdna:known chromosome:CEL140:II:14114141:14122056:-1 gene:K09E4.4 11/11 A 
K09E4.4.1 ENSEMBL cdna:known chromosome:CEL140:II:14114141:14118001:-1 gene:K09E4.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:14114186-14117259(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl K09E4.4
Entrez Gene 175063 Entrez gene
SwissProt Q9NAP6 EMBL-EBI
WormBase CE32692 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB70170 Hypothetical protein K09E4.4 [Caenorhabditis elegans] ref|NP_496948.2| K09E4.4 [Caenorhabditis elegans] 0.0
blast CAE73404 Hypothetical protein CBG20846 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR007781 EMBL-EBI Alpha-N-acetylglucosaminidase 5.8E-124
Pfam IPR007781 EMBL-EBI Alpha-N-acetylglucosaminidase 1.3E-11
Pfam IPR007781 EMBL-EBI Alpha-N-acetylglucosaminidase 2.3E-105
Pfam IPR007781 EMBL-EBI Alpha-N-acetylglucosaminidase 1.3E-11
Pfam IPR007781 EMBL-EBI Alpha-N-acetylglucosaminidase 6.4E-119
Pfam IPR007781 EMBL-EBI Alpha-N-acetylglucosaminidase 1.3E-11
Sequence
Target Sequence 
>C. ELEGANS:178331_AT
gcacattctacaatcagcccgagaaatggggaacgccaagattttcagtattcctatacc
atagacctggatttggaagaaaaatagagtattggttcccggtagaagagacgttttcga
gattccgagagcttctgccagcgctggtccatgtgctcggcgagcatcctctgttcagag
aagacctgaacgacgtgatgcgggaaatgacacaattcgagatgggaaatgaggcggctt
tatcaatgtcagaagcatttttgatggaagataagcagcaagttggcgccagttgtgaga
tgttgatggaaatgttccagaagttggaaagttactcgaatcgggatgttcggcagtgga
tagaaaatgcaaaatccatcgccccaacatcagaagaacgtcaagtgttcccggtgaccg
ccggtgacatcctaacagtctggggtccgactggtcaaaatctggactatgctcatcgcg
agtgggctgggcttatgtcgggctactacggacggcgatggcaatacttttgtgattgga
ttctcgagcatgatgatttcaaccacaccgaattttcgatctcagtttttcgtgacgta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCACATTCTACAATCAGCCCGAGAA 245 317 1112 Antisense
AACGCCAAGATTTTCAGTATTCCTA 91 147 1143 Antisense
GTATTCCTATACCATAGACCTGGAT 389 451 1159 Antisense
GAGTATTGGTTCCCGGTAGAAGAGA 362 397 1198 Antisense
TTCGAGATTCCGAGAGCTTCTGCCA 174 697 1227 Antisense
GGCGAGCATCCTCTGTTCAGAGAAG 459 539 1270 Antisense
GAAGACCTGAACGACGTGATGCGGG 413 339 1291 Antisense
GAGGCGGCTTTATCAATGTCAGAAG 163 405 1342 Antisense
GATAAGCAGCAAGTTGGCGCCAGTT 433 421 1381 Antisense
GTTGGCGCCAGTTGTGAGATGTTGA 516 435 1393 Antisense
TTCGATCTCAGTTTTTCGTGACGTA 494 695 1686 Antisense
Sequence Source Affymetrix Proprietary Database