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[178325_at]

Description R05D7.2 /REP_DB=WormBase Gene ID /WP=CE16296 /TR=O45706 /GB=CAB03216.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 19      4grp_total > 16      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 85
1 2 3 4 range
A 7.2
(-3)
A 		48.5
(1.6)
A 		41.5
(0.8)
A 		60.8
(3)
A 		54
B 14.4
(1.1)
A 		8.5
(-0.9)
A 		19.8
(3)
A 		2.5
(-3)
A 		17
C 42.4
(-1.6)
A 		28.9
(-3)
A 		33.3
(-2.6)
A 		87.7
(3)
A 		59
avr 21.3
(-3) 		28.6
(-1.5) 		31.5
(-0.9) 		50.3
(3) 		29

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 73
1 2 3 4 5 6 range
A 88.1
(3)
P 		21.7
(-3)
A 		52.4
(-0.2)
A 		37
(-1.6)
A 		39.4
(-1.4)
A 		22
(-3)
A 		66
B 47.5
(1.2)
A 		14.8
(-3)
A 		27.9
(-1.3)
A 		34.8
(-0.4)
A 		38
(0)
A 		61.1
(3)
P 		46
C 57.1
(3)
P 		33.7
(-0.9)
A 		53.6
(2.4)
A 		21
(-3)
A 		47.3
(1.4)
P 		55.7
(2.8)
P 		36
avr 64.2
(3) 		23.4
(-3) 		44.6
(0.1) 		30.9
(-1.9) 		41.6
(-0.3) 		46.3
(0.4) 		41
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 14
1 2 3 4 range
A 2.6
(-3)
A 		10.5
(2.8)
A 		10.8
(3)
A 		8.9
(1.6)
A 		8
B 4.6
(3)
A 		2.1
(-0.7)
A 		4.6
(3)
A 		0.5
(-3)
A 		4
C 14
(2.3)
A 		8.3
(-3)
A 		11.6
(0)
A 		14.8
(3)
A 		7
avr 7.1
(-2.7) 		7
(-3) 		9
(3) 		8.1
(0.3) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 19
1 2 3 4 5 6 range
A 16.2
(3)
P 		7.7
(-1.7)
A 		11.4
(0.3)
A 		9.4
(-0.8)
A 		11.9
(0.6)
A 		5.4
(-3)
A 		11
B 16.5
(0.9)
A 		4.9
(-3)
A 		6.8
(-2.4)
A 		9.2
(-1.6)
A 		15.5
(0.6)
A 		22.8
(3)
P 		18
C 21.2
(3)
P 		10.3
(-0.8)
A 		13.6
(0.3)
A 		4.2
(-3)
A 		15.3
(0.9)
P 		18.6
(2.1)
P 		17
avr 18
(3) 		7.6
(-3) 		10.6
(-1.3) 		7.6
(-3) 		14.2
(0.8) 		15.6
(1.6) 		10
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178325_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2257
Sequence Type Exemplar sequence
Representative Public ID R05D7.2 NCBI
Target Description R05D7.2 /REP_DB=WormBase Gene ID /WP=CE16296 /TR=O45706 /GB=CAB03216.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060674(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060674 NCBI Caenorhabditis elegans R05D7.2 (R05D7.2) mRNA, complete cds. 11/11 None
SNAP00000020572 ENSEMBL cdna:SNAP chromosome:CEL140:I:12169301:12176144:1 11/11 None
GENEFINDER00000020580 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:12173851:12176144:1 11/11 None
R05D7.2 ENSEMBL cdna:known chromosome:CEL140:I:12173851:12176070:1 gene:R05D7.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:12173781-12176001(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl R05D7.2
Entrez Gene 187604 Entrez gene
SwissProt O45706 EMBL-EBI
WormBase CE16296 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB03216 Hypothetical protein R05D7.2 [Caenorhabditis elegans] ref|NP_493075.1| R05D7.2 [Caenorhabditis elegans] 1.0E-161
blast CAB03216 Hypothetical protein R05D7.2 [Caenorhabditis elegans] ref|NP_493075.1| R05D7.2 [Caenorhabditis elegans] 1.0E-156
blast CAB03216 Hypothetical protein R05D7.2 [Caenorhabditis elegans] ref|NP_493075.1| R05D7.2 [Caenorhabditis elegans] 1.0E-155
blast CAB07288 Hypothetical protein T28A8.4 [Caenorhabditis elegans] ref|NP_499794.1| T28A8.4 [Caenorhabditis elegans] 7.0E-29
blast CAB07288 Hypothetical protein T28A8.4 [Caenorhabditis elegans] ref|NP_499794.1| T28A8.4 [Caenorhabditis elegans] 9.0E-29
blast CAB07288 Hypothetical protein T28A8.4 [Caenorhabditis elegans] ref|NP_499794.1| T28A8.4 [Caenorhabditis elegans] 2.0E-27
blast CAB55013 Hypothetical protein Y57A10A.8 [Caenorhabditis elegans] ref|NP_496588.2| Y57A10A.8 [Caenorhabditis elegans] 1.0E-21
Protein Domains
Database ID Description E-Value
Pfam IPR006570 EMBL-EBI SPK 4.4E-52
Pfam IPR006570 EMBL-EBI SPK 4.4E-52
Sequence
Target Sequence 
>C. ELEGANS:178325_AT
tgaaggatgtcatagccggtcggcaaagataagaagaagacatattgaaaaactgaaaaa
gccaaacattgttagaacagatgatttcgatgtgaaaatggaggaagaaatcgagcagga
gcctgaggatcttcctgtggaggttgcacttcagccgaatgaaataagtttgcacaaact
tttgaaacaaatgagacaaatgctcgtgtttctggattcgaacggattcacatcaatcag
gcagaaaatcgacgattcgattaaattttcacgcgatgaggcactcccagattcactaat
cgttctgactcttgacggaatgttcactggagcttctcgtaaattccgaccgtccgtgga
tccttctcgagcacaacaacatgatcaatggaatgctcgagtgttgttgattgtatatct
ttgttttttatacgcgacagattcaccgcgtcttaagaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGAAGGATGTCATAGCCGGTCGGCA 494 575 384 Antisense
CGAGCAGGAGCCTGAGGATCTTCCT 220 251 495 Antisense
GATCTTCCTGTGGAGGTTGCACTTC 432 419 511 Antisense
GACAAATGCTCGTGTTTCTGGATTC 158 365 578 Antisense
AAATTTTCACGCGATGAGGCACTCC 651 115 646 Antisense
GCACTCCCAGATTCACTAATCGTTC 51 317 664 Antisense
TAATCGTTCTGACTCTTGACGGAAT 238 635 680 Antisense
GACGGAATGTTCACTGGAGCTTCTC 621 375 697 Antisense
TGGAGCTTCTCGTAAATTCCGACCG 522 549 711 Antisense
ATCCTTCTCGAGCACAACAACATGA 221 39 743 Antisense
CGACAGATTCACCGCGTCTTAAGAA 147 251 818 Antisense
Sequence Source Affymetrix Proprietary Database