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[178322_at]

Description ZK856.5 /REP_DB=WormBase Gene ID /WP=CE06662 /TR=Q23640 /GB=CAA94853.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 4grp_high_8_8 > 24      6grp_total > 51      4grp_total > 39      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 173
1 2 3 4 range
A 234.7
(3)
P 		86.2
(-3)
M 		129.6
(-1.2)
P 		162.8
(0.1)
P 		149
B 245.4
(3)
P 		72.9
(-3)
P 		82.3
(-2.7)
A 		127.4
(-1.1)
P 		173
C 191.4
(3)
P 		103.8
(-3)
P 		107.6
(-2.7)
P 		125.9
(-1.5)
P 		88
avr 223.8
(3) 		87.6
(-3) 		106.5
(-2.2) 		138.7
(-0.7) 		136

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 200
1 2 3 4 5 6 range
A 146.7
(-0.2)
P 		160.5
(0.3)
P 		223.6
(3)
P 		206.9
(2.3)
P 		150.4
(-0.1)
P 		80.9
(-3)
A 		143
B 105
(-0.3)
P 		132
(0.6)
P 		204.7
(3)
P 		200.5
(2.9)
P 		122.9
(0.3)
P 		23.9
(-3)
A 		181
C 111.4
(-0.9)
P 		183.8
(1.8)
P 		214.6
(3)
P 		211.1
(2.9)
A 		98
(-1.5)
A 		57.4
(-3)
A 		157
avr 121
(-0.5) 		158.8
(0.9) 		214.3
(3) 		206.2
(2.7) 		123.8
(-0.4) 		54.1
(-3) 		160
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 67
1 2 3 4 range
A 85.4
(3)
P 		18.7
(-3)
M 		33.9
(-1.6)
P 		23.8
(-2.5)
P 		67
B 78.3
(3)
P 		18
(-3)
P 		19.1
(-2.9)
A 		27.1
(-2.1)
P 		60
C 63.4
(3)
P 		29.8
(-1.8)
P 		37.5
(-0.7)
P 		21.2
(-3)
P 		42
avr 75.7
(3) 		22.2
(-3) 		30.2
(-2.1) 		24
(-2.8) 		54

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 48
1 2 3 4 5 6 range
A 27
(-1.8)
P 		57.1
(3)
P 		48.7
(1.7)
P 		52.5
(2.3)
P 		45.4
(1.1)
P 		19.7
(-3)
A 		37
B 36.5
(0.7)
P 		43.9
(1.8)
P 		49.7
(2.5)
P 		53.1
(3)
P 		49.9
(2.6)
P 		8.9
(-3)
A 		44
C 41.3
(0.6)
P 		56.1
(3)
P 		54.6
(2.8)
P 		42.5
(0.8)
A 		31.7
(-1)
A 		19.2
(-3)
A 		37
avr 34.9
(0.1) 		52.4
(3) 		51
(2.8) 		49.4
(2.5) 		42.3
(1.3) 		15.9
(-3) 		37
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178322_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12216
Sequence Type Exemplar sequence
Representative Public ID ZK856.5 NCBI
Target Description ZK856.5 /REP_DB=WormBase Gene ID /WP=CE06662 /TR=Q23640 /GB=CAA94853.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073219(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073219 NCBI Caenorhabditis elegans ZK856.5 (ZK856.5) mRNA, complete cds. 9/11 None
GENEFINDER00000036976 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:10191714:10192698:-1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000000586 1/11 Cross Hyb Matching Probes None
GENEFINDER00000008160 1/11 Cross Hyb Matching Probes None
GENEFINDER00000019531 1/11 Cross Hyb Matching Probes None
GENEFINDER00000025865 1/11 Cross Hyb Matching Probes None
SNAP00000036947 8/11 Cross Hyb Matching Probes None
ZK856.5 8/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:10191713-10192698(-) 73.13 100.0
Public Domain and Genome References
Gene Title
Entrez Gene 191439 Entrez gene
SwissProt Q23640 EMBL-EBI
WormBase CE38478 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.18594.1.S1_AT similar to acid sphingomyelinase-like phosphodiesterase 3B isoform 1 cfa
DROSOPHILA_2:1638906_AT dm
CHICKEN:GGA.6971.1.S1_AT sphingomyelin phosphodiesterase, acid-like 3B gga
CHICKEN:GGA.6971.1.S1_S_AT sphingomyelin phosphodiesterase, acid-like 3B gga
CHICKEN:GGAAFFX.21962.1.S1_AT sphingomyelin phosphodiesterase, acid-like 3B gga
CHICKEN:GGA.6971.2.S1_AT Sphingomyelin phosphodiesterase, acid-like 3B gga
CHICKEN:GGA.6971.2.A1_AT Sphingomyelin phosphodiesterase, acid-like 3B gga
HG-U133_PLUS_2:205309_AT sphingomyelin phosphodiesterase, acid-like 3B hs
HG-U133A:205309_AT sphingomyelin phosphodiesterase, acid-like 3B hs
HG-FOCUS:205309_AT sphingomyelin phosphodiesterase, acid-like 3B hs
HG-U133A_2:205309_AT sphingomyelin phosphodiesterase, acid-like 3B hs
HU35KSUBA:Y08134_AT sphingomyelin phosphodiesterase, acid-like 3B hs
HUGENEFL:Y08134_AT sphingomyelin phosphodiesterase, acid-like 3B hs
U133_X3P:G7656907_3P_AT sphingomyelin phosphodiesterase, acid-like 3B hs
U133_X3P:HS2.123659.2.S1_3P_AT sphingomyelin phosphodiesterase, acid-like 3B hs
HG-U133_PLUS_2:1554050_AT sphingomyelin phosphodiesterase, acid-like 3B hs
HG-U95AV2:37779_AT sphingomyelin phosphodiesterase, acid-like 3B hs
HG-U95C:64012_AT sphingomyelin phosphodiesterase, acid-like 3B hs
MG-U74BV2:115354_AT sphingomyelin phosphodiesterase, acid-like 3B mm
MOE430A:1417300_AT sphingomyelin phosphodiesterase, acid-like 3B mm
MOUSE430A_2:1417300_AT sphingomyelin phosphodiesterase, acid-like 3B mm
MOUSE430_2:1417300_AT sphingomyelin phosphodiesterase, acid-like 3B mm
Protein Similarities
Method ID Description E-Value
blast CAA94853 Hypothetical protein ZK856.5 [Caenorhabditis elegans] ref|NP_505620.3| ZK856.5 [Caenorhabditis elegans] 0.0
blast T28041 hypothetical protein ZK856.3 - Caenorhabditis elegans 0.0
blast CAE64751 Hypothetical protein CBG09540 [Caenorhabditis briggsae] 0.0
blast CAA94853 Hypothetical protein ZK856.5 [Caenorhabditis elegans] ref|NP_505620.3| ZK856.5 [Caenorhabditis elegans] 2.0E-60
blast CAI14295 sphingomyelin phosphodiesterase, acid-like 3B [Homo sapiens] 9.0E-13
blast NP_055289 acid sphingomyelinase-like phosphodiesterase 3B isoform 1 [Homo sapiens] emb|CAI14293.1| sphingomyelin phosphodiesterase, acid-like 3B [Homo sapiens] sp|Q92485|ASM3B_HUMAN Acid sphingomyelinase-like phosphodiesterase 3b precursor (ASM-like phosphodiesterase 3b) 9.0E-13
Protein Domains
Database ID Description E-Value
Pfam IPR004843 EMBL-EBI Metallo-phosphoesterase 8.0E-12
Sequence
Target Sequence 
>C. ELEGANS:178322_AT
gtggcccaacttgttaatgtacctactttttctggctctacctctcaaatcctggggcta
tcaagttcttcatctcgccgattttcatcttgacgttcaatattctataaacggagacaa
taaacatatgtgtcacgatgatggtacggaacgaaatacaacacttggagagtatggaga
ctacatgtgtgatgcaccaaaaccactgatccaactcgctatcgatgaatctgcccgtat
atttccaaatccggatcttatcatttggacaggagataatgtggctcacattgatggata
tggatgggaaattgttcttgatgccgtcaatcaaaccacctcgcttcttttctccaggta
cagtgctggccaaaaagatatccacttttggttttttgtgtgtaacttttttctcaagca
tccatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGGCCCAACTTGTTAATGTACCTA 332 485 15 Antisense
AATGTACCTACTTTTTCTGGCTCTA 685 161 30 Antisense
CTACCTCTCAAATCCTGGGGCTATC 447 225 52 Antisense
GGCTATCAAGTTCTTCATCTCGCCG 40 539 70 Antisense
TCGCCGATTTTCATCTTGACGTTCA 488 599 89 Antisense
GTGATGCACCAAAACCACTGATCCA 283 483 203 Antisense
CACTGATCCAACTCGCTATCGATGA 148 199 218 Antisense
AATCTGCCCGTATATTTCCAAATCC 342 169 242 Antisense
GGGAAATTGTTCTTGATGCCGTCAA 325 495 320 Antisense
CTTCTTTTCTCCAGGTACAGTGCTG 298 221 358 Antisense
GTAACTTTTTTCTCAAGCATCCATT 699 461 416 Antisense
Sequence Source Affymetrix Proprietary Database