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[178312_at]

Description T27C5.8 /REP_DB=WormBase Gene ID /WP=CE16502 /TR=O45852 /GB=CAB04869.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 58      4grp_total > 32      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 69
1 2 3 4 range
A 19.9
(-3)
A 		35.5
(1.5)
A 		40.7
(3)
A 		21.7
(-2.5)
A 		21
B 24.9
(1.5)
A 		9.1
(-1.9)
A 		31.8
(3)
A 		4
(-3)
A 		28
C 42.5
(-1.3)
A 		30.7
(-3)
A 		39.4
(-1.8)
A 		73.2
(3)
A 		43
avr 29.1
(-1) 		25.1
(-3) 		37.3
(3) 		33
(0.9) 		12

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 84
1 2 3 4 5 6 range
A 87.5
(2.7)
A 		21.1
(-2)
A 		34.8
(-1)
A 		7.4
(-3)
A 		26.7
(-1.6)
A 		91.2
(3)
A 		84
B 28.9
(-1.1)
A 		30
(-1)
A 		43.1
(0.4)
A 		11.2
(-3)
A 		17.7
(-2.3)
A 		67
(3)
P 		56
C 33.6
(-2.1)
A 		56.7
(0.3)
A 		24.5
(-3)
A 		36.6
(-1.8)
A 		33.7
(-2)
A 		82.6
(3)
P 		58
avr 50
(0.1) 		35.9
(-1.3) 		34.1
(-1.5) 		18.4
(-3) 		26
(-2.3) 		80.3
(3) 		62
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 13
1 2 3 4 range
A 7.2
(0.2)
A 		7.7
(0.6)
A 		10.6
(3)
A 		3.2
(-3)
A 		7
B 7.9
(3)
A 		2.2
(-1.9)
A 		7.4
(2.6)
A 		0.9
(-3)
A 		7
C 14.1
(3)
A 		8.8
(-3)
A 		13.7
(2.5)
A 		12.3
(1)
A 		5
avr 9.7
(1.9) 		6.2
(-2.2) 		10.6
(3) 		5.5
(-3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 26
1 2 3 4 5 6 range
A 16.1
(1.2)
A 		7.5
(-1.3)
A 		7.6
(-1.3)
A 		1.9
(-3)
A 		8
(-1.2)
A 		22.2
(3)
A 		20
B 10.1
(-1.1)
A 		10
(-1.1)
A 		10.5
(-1)
A 		3
(-3)
A 		7.2
(-1.9)
A 		25
(3)
P 		22
C 12.5
(-1.2)
A 		17.3
(0.1)
A 		6.2
(-3)
A 		7.4
(-2.7)
A 		10.9
(-1.7)
A 		27.6
(3)
P 		21
avr 12.9
(-0.5) 		11.6
(-0.8) 		8.1
(-1.8) 		4.1
(-3) 		8.7
(-1.7) 		24.9
(3) 		21
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178312_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14590
Sequence Type Exemplar sequence
Representative Public ID T27C5.8 NCBI
Target Description T27C5.8 /REP_DB=WormBase Gene ID /WP=CE16502 /TR=O45852 /GB=CAB04869.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075006(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075006 NCBI Caenorhabditis elegans T27C5.8 (T27C5.8) mRNA, complete cds. 11/11 None
GENEFINDER00000030304 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:17419734:17420968:1 11/11 None
T27C5.8 ENSEMBL cdna:known chromosome:CEL140:V:17419734:17420968:1 gene:T27C5.8 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000030297 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:17419733-17420968(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T27C5.8
Entrez Gene 188987 Entrez gene
SwissProt O45852 EMBL-EBI
WormBase CE16502 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04869 Hypothetical protein T27C5.8 [Caenorhabditis elegans] ref|NP_507407.1| T27C5.8 [Caenorhabditis elegans] 2.0E-99
blast CAB04447 Hypothetical protein F49H6.3 [Caenorhabditis elegans] ref|NP_507307.1| F49H6.3 [Caenorhabditis elegans] 2.0E-68
blast CAA20971 Hypothetical protein F49H6.13 [Caenorhabditis elegans] emb|CAB04448.1| Hypothetical protein F49H6.13 [Caenorhabditis elegans] ref|NP_507304.1| F49H6.13 [Caenorhabditis elegans] 1.0E-59
Protein Domains
Database ID Description E-Value
Pfam IPR009545 EMBL-EBI Protein of unknown function DUF1164 8.6E-90
Pfam IPR009545 EMBL-EBI Protein of unknown function DUF1164 5.2E-18
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_507407.1 4 7-29,49-71,92-114,158-180
T27C5.8 4 7-29,49-71,92-114,158-180
GENEFINDER00000030304 4 7-29,49-71,92-114,158-180
Sequence
Target Sequence 
>C. ELEGANS:178312_AT
agcgtttgttctttactgtagtcaccattgacgcaatgacctgcaacttttttggtgtac
tctcgatcgcttgggtaacagattggagtggaagcatcgggctttttccactttgggtcg
tcaccattggatggtatgtcgctgcgagtttggtgatggaagtctccctcctttgtaccg
tcttagtgctcattcagctgatagtcacatcaaataccatcagaaggaacggttactctc
ccacgcagagagggaaatttatttcaattgctgttctgtcactgttcatcactgttttaa
cagttgttggtgttactttaattggagttaacttgccacatatgaacaggaagtataatg
ataatgctacaccagccaaaactcctaacctatgttttactatattacaaggtttaacta
tgtatgttactgtagggaacgctaagagtactcttggatactctgcatggatcagtgtag
ccggtgcagttttctacctcgttgttgcgggattatcgattagctacgcatgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGCGTTTGTTCTTTACTGTAGTCAC 643 75 26 Antisense
GACGCAATGACCTGCAACTTTTTTG 551 377 55 Antisense
GCAACTTTTTTGGTGTACTCTCGAT 642 317 68 Antisense
TGTACTCTCGATCGCTTGGGTAACA 35 561 81 Antisense
CAGAAGGAACGGTTACTCTCCCACG 80 205 246 Antisense
TGGAGTTAACTTGCCACATATGAAC 135 549 348 Antisense
AATGCTACACCAGCCAAAACTCCTA 560 165 388 Antisense
CCAGCCAAAACTCCTAACCTATGTT 548 273 397 Antisense
AGTGTAGCCGGTGCAGTTTTCTACC 528 1 499 Antisense
TTTTCTACCTCGTTGTTGCGGGATT 625 671 515 Antisense
GGGATTATCGATTAGCTACGCATGT 274 497 534 Antisense
Sequence Source Affymetrix Proprietary Database