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[178306_at]

Description R04B5.1 /REP_DB=WormBase Gene ID /WP=CE06212 /TR=Q21699 /GB=CAA94838.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 98      4grp_total > 36      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 37
1 2 3 4 range
A 19.6
(3)
A 		16.5
(1.7)
A 		4.9
(-3)
A 		18.3
(2.5)
A 		15
B 4.2
(-2.9)
A 		38.5
(3)
A 		3.7
(-3)
A 		4.1
(-2.9)
A 		35
C 18.4
(0.6)
A 		15.3
(-0.1)
A 		1.1
(-3)
A 		30.2
(3)
P 		29
avr 14.1
(0.2) 		23.4
(3) 		3.2
(-3) 		17.5
(1.2) 		20

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 68
1 2 3 4 5 6 range
A 53.9
(1.6)
A 		2.5
(-3)
A 		53.3
(1.5)
A 		18.7
(-1.6)
A 		44.5
(0.7)
A 		70.1
(3)
P 		68
B 12.1
(-2.1)
A 		8.2
(-2.7)
A 		6.6
(-3)
A 		9.8
(-2.5)
A 		44.7
(3)
P 		38.8
(2.1)
P 		38
C 2.5
(-3)
A 		19.7
(-0.1)
P 		29.6
(1.6)
A 		15
(-0.9)
A 		29.7
(1.6)
A 		38.1
(3)
A 		36
avr 22.8
(-1) 		10.1
(-3) 		29.8
(0) 		14.5
(-2.3) 		39.6
(1.6) 		49
(3) 		39
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 9
1 2 3 4 range
A 7.1
(3)
A 		3.6
(-0.6)
A 		1.3
(-3)
A 		2.7
(-1.6)
A 		6
B 1.3
(-2.7)
A 		9.5
(3)
A 		0.9
(-3)
A 		0.9
(-3)
A 		9
C 6.1
(3)
A 		4.4
(1.2)
A 		0.4
(-3)
A 		5.1
(1.9)
P 		6
avr 4.8
(1.8) 		5.8
(3) 		0.9
(-3) 		2.9
(-0.6) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 17
1 2 3 4 5 6 range
A 9.9
(0.3)
A 		0.9
(-3)
A 		11.6
(1)
A 		4.7
(-1.6)
A 		13.4
(1.6)
A 		17.1
(3)
P 		16
B 4.2
(-2.1)
A 		2.7
(-2.6)
A 		1.6
(-3)
A 		2.6
(-2.6)
A 		18.2
(3)
P 		14.5
(1.7)
P 		17
C 0.9
(-3)
A 		6
(-0.4)
P 		7.5
(0.3)
A 		3
(-1.9)
A 		9.6
(1.4)
A 		12.8
(3)
A 		12
avr 5
(-2.1) 		3.2
(-3) 		6.9
(-1.1) 		3.4
(-2.9) 		13.7
(2.4) 		14.8
(3) 		12
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178306_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11940
Sequence Type Exemplar sequence
Representative Public ID R04B5.1 NCBI
Target Description R04B5.1 /REP_DB=WormBase Gene ID /WP=CE06212 /TR=Q21699 /GB=CAA94838.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073186(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073186 NCBI Caenorhabditis elegans R04B5.1 (R04B5.1) mRNA, complete cds. 11/11 None
SNAP00000020531 ENSEMBL cdna:SNAP chromosome:CEL140:V:10080500:10080858:-1 11/11 None
R04B5.1 ENSEMBL cdna:known chromosome:CEL140:V:10080500:10080858:-1 gene:R04B5.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:10080499-10080858(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl R04B5.1
Entrez Gene 187566 Entrez gene
SwissProt Q21699 EMBL-EBI
WormBase CE06212 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA94838 Hypothetical protein R04B5.1 [Caenorhabditis elegans] ref|NP_505587.1| R04B5.1 [Caenorhabditis elegans] 6.0E-42
blast CAE64013 Hypothetical protein CBG08608 [Caenorhabditis briggsae] 2.0E-27
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_505587.1 1 15-33
R04B5.1 1 15-33
SNAP00000020531 1 15-33
Sequence
Target Sequence 
>C. ELEGANS:178306_AT
gtacgaccaggaaaatctcattgttgacagaagtgagcactttgcattgctttttggtgc
gttttccatgggaataatcgttcttatttttgcactccgcggacaaagaaatcggatttt
cgaagagaacatgttcaagcagagaatacttttgagccagattttcgatatgtacatggt
aagaacagagtctttgcgaattcgtcaaaagcagctcgaggctcatcgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTACGACCAGGAAAATCTCATTGTT 360 459 15 Antisense
GAAGTGAGCACTTTGCATTGCTTTT 657 335 44 Antisense
GCATTGCTTTTTGGTGCGTTTTCCA 417 307 58 Antisense
GCTTTTTGGTGCGTTTTCCATGGGA 514 303 63 Antisense
TTTTCCATGGGAATAATCGTTCTTA 101 677 76 Antisense
TTATTTTTGCACTCCGCGGACAAAG 529 675 98 Antisense
AGCAGAGAATACTTTTGAGCCAGAT 475 77 152 Antisense
TGAGCCAGATTTTCGATATGTACAT 468 569 167 Antisense
GAACAGAGTCTTTGCGAATTCGTCA 574 351 197 Antisense
GAATTCGTCAAAAGCAGCTCGAGGC 8 323 212 Antisense
TCAAAAGCAGCTCGAGGCTCATCGC 215 629 219 Antisense
Sequence Source Affymetrix Proprietary Database