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[178302_at]

Description F58B4.5 /REP_DB=WormBase Gene ID /WP=CE06015 /TR=Q20974 /GB=CAA98496.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 21      6grp_high1_10_10 > 02      4grp_highest_8_8 > 01      4grp_high_8_8 > 45      6grp_highest_nonorm > 60      6grp_total > 23      4grp_total > 51      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 781
1 2 3 4 range
A 643.7
(-1)
P 		1104.9
(3)
P 		764.3
(0)
P 		417.8
(-3)
P 		687
B 700.5
(-0.7)
P 		1147.7
(3)
P 		839.6
(0.4)
P 		425.9
(-3)
P 		722
C 555.3
(-1.3)
P 		1021.2
(3)
P 		1001.4
(2.8)
P 		367.1
(-3)
P 		654
avr 633.2
(-1) 		1091.3
(3) 		868.4
(1.1) 		403.6
(-3) 		688

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 609
1 2 3 4 5 6 range
A 580.7
(3)
P 		502.9
(1.9)
P 		545.2
(2.5)
P 		373.7
(0.1)
P 		233.3
(-1.8)
P 		145.7
(-3)
P 		435
B 703.5
(3)
P 		514.6
(0.9)
P 		464.4
(0.4)
P 		282.3
(-1.6)
P 		232.4
(-2.2)
P 		157.4
(-3)
P 		546
C 754.6
(3)
P 		548.2
(0.8)
P 		530.4
(0.6)
P 		417.6
(-0.5)
P 		214.5
(-2.7)
P 		184.9
(-3)
P 		570
avr 679.6
(3) 		521.9
(1.2) 		513.3
(1.1) 		357.9
(-0.7) 		226.7
(-2.3) 		162.7
(-3) 		517
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 288
1 2 3 4 range
A 234.4
(2.8)
P 		239.8
(3)
P 		199.8
(1.7)
P 		61.2
(-3)
P 		179
B 223.4
(1.1)
P 		283.5
(3)
P 		194.6
(0.2)
P 		90.8
(-3)
P 		193
C 183.8
(-0.5)
P 		293.6
(1.8)
P 		349
(3)
P 		61.9
(-3)
P 		287
avr 213.9
(1.3) 		272.3
(3) 		247.8
(2.3) 		71.3
(-3) 		201

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 244
1 2 3 4 5 6 range
A 107
(-0)
P 		178.9
(3)
P 		118.7
(0.5)
P 		94.8
(-0.5)
P 		70.4
(-1.5)
P 		35.5
(-3)
P 		143
B 244.8
(3)
P 		171.3
(0.6)
P 		112.8
(-1.3)
P 		74.8
(-2.5)
P 		94.4
(-1.8)
P 		58.7
(-3)
P 		186
C 279.7
(3)
P 		167.3
(-0.1)
P 		135
(-1)
P 		84
(-2.4)
P 		69.4
(-2.8)
P 		61.9
(-3)
P 		218
avr 210.5
(3) 		172.5
(1.6) 		122.2
(-0.3) 		84.5
(-1.8) 		78.1
(-2) 		52
(-3) 		159
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178302_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14138
Sequence Type Exemplar sequence
Representative Public ID F58B4.5 NCBI
Target Description F58B4.5 /REP_DB=WormBase Gene ID /WP=CE06015 /TR=Q20974 /GB=CAA98496.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073387(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073387 NCBI Caenorhabditis elegans F58B4.5 (F58B4.5) mRNA, complete cds. 11/11 None
SNAP00000018000 ENSEMBL cdna:SNAP chromosome:CEL140:V:10935327:10936790:1 11/11 None
GENEFINDER00000018004 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:10935327:10936790:1 11/11 None
F58B4.5 ENSEMBL cdna:known chromosome:CEL140:V:10935234:10936825:1 gene:F58B4.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:10935326-10936790(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F58B4.5
Entrez Gene 179513 Entrez gene
SwissProt Q20974 EMBL-EBI
WormBase CE06015 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA98496 Hypothetical protein F58B4.5 [Caenorhabditis elegans] ref|NP_505788.1| F58B4.5 [Caenorhabditis elegans] 0.0
blast CAE75124 Hypothetical protein CBG23052 [Caenorhabditis briggsae] 0.0
blast CAE72164 Hypothetical protein CBG19264 [Caenorhabditis briggsae] 7.0E-98
Sequence
Target Sequence 
>C. ELEGANS:178302_AT
tactgcttaaagcgtcattcccagaagagtatttggagaaagttgaggaaatgctgaaaa
tctacaaagactattatttccaaccgcaaatgatcaaaaatttcaaaaatacatgccaat
tctttggttataaaccagttctcacccattctgatttgtggagctcaaactttttgtgta
ctcgagatggagaaaaagttacattgaaagcgattattgatttccagacagtttcaatca
caactccagcacaagacgttggccgattgtttgcatcatgcttatcaacaaaagatagaa
gagaaaaggcagatttcttgttggaagaatactacaatacatttgtaaatgaacttgatg
gaatggatgttccatatacattccaacaacttaaagatagttatcaagtttacttcccac
taatgactacaatggttcttcctggaattgcaccaatgctccaacattcaaatgtaactg
aagaatacaaagactctatgaaacaagtggcactagacaaaatgattggacttcttgaag
atgttatcacaactcacgagagcaata

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TACTGCTTAAAGCGTCATTCCCAGA 502 647 686 Antisense
CAAAGACTATTATTTCCAACCGCAA 658 189 750 Antisense
GTTATAAACCAGTTCTCACCCATTC 553 447 812 Antisense
GTTCTCACCCATTCTGATTTGTGGA 527 443 823 Antisense
GATTTGTGGAGCTCAAACTTTTTGT 549 427 838 Antisense
AACTTTTTGTGTACTCGAGATGGAG 602 139 853 Antisense
AAGCGATTATTGATTTCCAGACAGT 27 151 893 Antisense
TTCAATCACAACTCCAGCACAAGAC 409 685 918 Antisense
ACTCCAGCACAAGACGTTGGCCGAT 126 99 928 Antisense
GTTGGCCGATTGTTTGCATCATGCT 144 435 943 Antisense
GTTATCACAACTCACGAGAGCAATA 464 447 1228 Antisense
Sequence Source Affymetrix Proprietary Database