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[178296_at]

Description F54B3.2 /REP_DB=WormBase Gene ID /WP=CE01590 /TR=Q09398 /GB=CAA88470.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 67      4grp_total > 01      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 60
1 2 3 4 range
A 1.8
(-3)
A 		3
(-2.9)
A 		7.6
(-2.4)
A 		56.6
(3)
A 		55
B 7
(-1.1)
A 		4.9
(-2.2)
A 		14.6
(3)
A 		3.4
(-3)
A 		11
C 17.6
(-1.6)
A 		8
(-2.6)
A 		4.6
(-3)
A 		61.3
(3)
A 		57
avr 8.8
(-2.4) 		5.3
(-3) 		8.9
(-2.4) 		40.4
(3) 		35

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 57
1 2 3 4 5 6 range
A 8.8
(-1.9)
A 		2
(-2.9)
A 		1.6
(-3)
A 		12.9
(-1.2)
A 		8.1
(-2)
A 		39.6
(3)
A 		38
B 6.5
(-0.8)
A 		4.7
(-1.7)
A 		6.5
(-0.8)
A 		1.8
(-3)
A 		6.9
(-0.6)
A 		14.7
(3)
A 		13
C 4.7
(-2.8)
A 		21.2
(-1.1)
A 		5
(-2.8)
A 		10.7
(-2.2)
A 		3.3
(-3)
A 		58.5
(3)
A 		55
avr 6.7
(-2.6) 		9.3
(-2.1) 		4.4
(-3) 		8.5
(-2.3) 		6.1
(-2.7) 		37.6
(3) 		33
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 10
1 2 3 4 range
A 0.6
(-3)
A 		0.6
(-3)
A 		2
(-1.9)
A 		8.3
(3)
A 		8
B 2.2
(0.3)
A 		1.2
(-1.9)
A 		3.4
(3)
A 		0.7
(-3)
A 		3
C 5.8
(-0.1)
A 		2.3
(-2.5)
A 		1.6
(-3)
A 		10.3
(3)
A 		9
avr 2.9
(-1.2) 		1.4
(-3) 		2.3
(-1.9) 		6.4
(3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 19
1 2 3 4 5 6 range
A 1.6
(-2.2)
A 		0.7
(-2.7)
A 		0.3
(-3)
A 		3.3
(-1.1)
A 		2.5
(-1.6)
A 		9.7
(3)
A 		9
B 2.2
(-1)
A 		1.6
(-1.7)
A 		1.6
(-1.7)
A 		0.5
(-3)
A 		2.8
(-0.2)
A 		5.5
(3)
A 		5
C 1.8
(-2.8)
A 		6.5
(-1.2)
A 		1.3
(-2.9)
A 		2.1
(-2.7)
A 		1.1
(-3)
A 		19.6
(3)
A 		19
avr 1.9
(-2.5) 		2.9
(-2) 		1.1
(-3) 		2
(-2.5) 		2.1
(-2.4) 		11.6
(3) 		11
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178296_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3348
Sequence Type Exemplar sequence
Representative Public ID F54B3.2 NCBI
Target Description F54B3.2 /REP_DB=WormBase Gene ID /WP=CE01590 /TR=Q09398 /GB=CAA88470.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063811(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063811 NCBI Caenorhabditis elegans F54B3.2 (F54B3.2) mRNA, complete cds. 11/11 None
SNAP00000006967 ENSEMBL cdna:SNAP chromosome:CEL140:II:10268084:10271193:-1 11/11 None
F54B3.2 ENSEMBL cdna:known chromosome:CEL140:II:10268084:10271060:-1 gene:F54B3.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000006969 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:10268080-10271057(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F54B3.2
Entrez Gene 186198 Entrez gene
SwissProt Q09398 EMBL-EBI
WormBase CE01590 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA88470 Hypothetical protein F54B3.2 [Caenorhabditis elegans] ref|NP_496212.1| F54B3.2 [Caenorhabditis elegans] 0.0
blast CAE56693 Hypothetical protein CBG24475 [Caenorhabditis briggsae] 2.0E-98
blast CAE57821 Hypothetical protein CBG00845 [Caenorhabditis briggsae] 9.0E-91
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_496212.1 1 252-274
F54B3.2 1 252-274
SNAP00000006967 1 252-274
Sequence
Target Sequence 
>C. ELEGANS:178296_AT
gaattccatcgtcgttcggtggtttcgaaaattcaaaaaactatcgaattcatgttgaaa
ctgaataatcagacatctacatactctcaactcattccatattggaatccaactattttc
ctaccacctgcttcatggcaatacagaaaattgggagaagagaaggagaagcttgaggct
gaaaatatgtcaatctcgtgggttctagtggtgtcagtaatgccaatgatattctttgga
gttgtcgtttcatgttatattgttattcagccacgtggtggcttgacatacactgacgaa
gaaattgacttctcaattcaagaagttgacagtgctagtcgtgtaaatgtacttcaacca
aagattcatccatttaaaattactccaattgtttatgtgaacccgactcattcgactgaa
accaattctattgatattgcaatggaaccaacaattcgacgcggtgatgctctgaaaacc
gctgtggatgatgatatacatactgacaagacgatgctctccgagggaaaatccacaaaa
accgttcggcttccacccaaggatactggtacgacttctgtggaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATTCCATCGTCGTTCGGTGGTTT 556 321 568 Antisense
ACATACTCTCAACTCATTCCATATT 583 105 646 Antisense
TGGAATCCAACTATTTTCCTACCAC 99 521 670 Antisense
CAATCTCGTGGGTTCTAGTGGTGTC 81 181 758 Antisense
GATATTCTTTGGAGTTGTCGTTTCA 131 427 795 Antisense
GCCACGTGGTGGCTTGACATACACT 108 279 837 Antisense
TGTGAACCCGACTCATTCGACTGAA 121 555 963 Antisense
ACAATTCGACGCGGTGATGCTCTGA 602 113 1018 Antisense
TACTGACAAGACGATGCTCTCCGAG 489 647 1068 Antisense
GGCTTCCACCCAAGGATACTGGTAC 112 537 1115 Antisense
GGATACTGGTACGACTTCTGTGGAA 81 511 1128 Antisense
Sequence Source Affymetrix Proprietary Database