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[178274_at]

Description T28D6.5 /REP_DB=WormBase Gene ID /WP=CE20112 /TR=O18151 /GB=CAB03450.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 26      4grp_total > 47      6grp_total_sub > Cup      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 287
1 2 3 4 range
A 332.1
(3)
P 		121.9
(-1.4)
A 		56.8
(-2.8)
A 		45.1
(-3)
A 		287
B 315.6
(3)
P 		91.4
(-3)
A 		90.7
(-3)
A 		168.9
(-0.9)
A 		225
C 311.3
(3)
P 		67.2
(-3)
A 		108.5
(-2)
A 		132.4
(-1.4)
A 		244
avr 319.7
(3) 		93.5
(-2.8) 		85.3
(-3) 		115.5
(-2.2) 		234

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 208
1 2 3 4 5 6 range
A 186.9
(0)
A 		244.6
(2.9)
M 		153.3
(-1.6)
A 		125.9
(-3)
A 		246.6
(3)
A 		209.4
(1.2)
A 		121
B 323.3
(2.7)
P 		334.1
(3)
P 		159.9
(-2.4)
A 		234.5
(-0.1)
A 		142.2
(-3)
A 		163.5
(-2.3)
A 		192
C 309.3
(3)
P 		277.8
(1.9)
M 		137.5
(-3)
A 		181
(-1.5)
A 		154.7
(-2.4)
A 		143.6
(-2.8)
A 		172
avr 273.2
(2.5) 		285.5
(3) 		150.2
(-3) 		180.5
(-1.7) 		181.2
(-1.6) 		172.2
(-2) 		135
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 114
1 2 3 4 range
A 120.9
(3)
P 		26.5
(-2)
A 		14.9
(-2.6)
A 		6.6
(-3)
A 		114
B 100.6
(3)
P 		22.6
(-2.9)
A 		21
(-3)
A 		36
(-1.9)
A 		80
C 103.1
(3)
P 		19.3
(-3)
A 		37.8
(-1.7)
A 		22.3
(-2.8)
A 		84
avr 108.2
(3) 		22.8
(-2.9) 		24.6
(-2.8) 		21.6
(-3) 		87

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 83
1 2 3 4 5 6 range
A 34.4
(-2.7)
A 		87
(3)
M 		33.4
(-2.8)
A 		31.9
(-3)
A 		74.4
(1.6)
A 		51
(-0.9)
A 		55
B 112.5
(3)
P 		111.2
(2.9)
P 		38.8
(-3)
A 		62.1
(-1.1)
A 		57.8
(-1.5)
A 		61
(-1.2)
A 		74
C 114.6
(3)
P 		84.8
(0.8)
M 		35
(-3)
A 		36.4
(-2.9)
A 		50
(-1.9)
A 		48.1
(-2)
A 		80
avr 87.2
(2.3) 		94.3
(3) 		35.7
(-3) 		43.5
(-2.2) 		60.7
(-0.4) 		53.4
(-1.2) 		59
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178274_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6285
Sequence Type Exemplar sequence
Representative Public ID T28D6.5 NCBI
Target Description T28D6.5 /REP_DB=WormBase Gene ID /WP=CE20112 /TR=O18151 /GB=CAB03450.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_181929(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_181929 NCBI Caenorhabditis elegans T28D6.5b (T28D6.5) mRNA, complete cds. 9/11 None
GENEFINDER00000023481 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:11337201:11341779:1 11/11 None
SNAP00000023469 ENSEMBL cdna:SNAP chromosome:CEL140:III:11338193:11341779:1 9/11 None
T28D6.5b ENSEMBL cdna:known chromosome:CEL140:III:11334703:11342104:1 gene:T28D6.5 9/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_067057 6/11 Cross Hyb Matching Probes None
T28D6.5a 6/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
176564 2 1 NM_181929 178274_at (9)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:11337196-11341775(+) 100.0 100.0
Public Domain and Genome References
Ensembl T28D6.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAD56611 Hypothetical protein T28D6.5b [Caenorhabditis elegans] emb|CAD56602.1| Hypothetical protein T28D6.5b [Caenorhabditis elegans] ref|NP_871658.1| T28D6.5b [Caenorhabditis elegans] 0.0
blast CAD56610 Hypothetical protein T28D6.5a [Caenorhabditis elegans] emb|CAB03450.2| Hypothetical protein T28D6.5a [Caenorhabditis elegans] ref|NP_499458.2| T28D6.5a [Caenorhabditis elegans] 0.0
blast CAE67643 Hypothetical protein CBG13202 [Caenorhabditis briggsae] 0.0
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_871658.1 1 369-391
T28D6.5b 1 369-391
GENEFINDER00000023481 1 273-295
SNAP00000023469 1 133-155
Sequence
Target Sequence 
>C. ELEGANS:178274_AT
ggatgcttccagggtcggaggatcagcaggatctcaaggatttgaacaagaaacgaacag
agacacctccatcctctccgattcagaatcatattcagaatcacttgaatcacaacaccg
agtctgattcggaagaagaggaagagcccgccagggtacaggatgacggaaaagacgccg
aaatcgccgaaaaagatgacgatttcgtgtacgaacgtctccgagaggaactttctcccc
agcctccgattgtcgtggatttagacactgacttagattttgtgccgatgaagcaaaaaa
actttgggatgaaaatgatgaacttggagccaaaatctgttatttcgaactccaagtctc
aatttccagcaagagtgggagaaatgagctctaaaacctccaaattttccagtcaaaaac
ctccaattttttcgccccaaaaacctcaattttccacccaaaacttccaatttttgcagt
cactaccaccaccgccaccaggaatgttccctgattctccaccagaatccggttcggga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGATGCTTCCAGGGTCGGAGGATCA 226 513 1634 Antisense
AAACGAACAGAGACACCTCCATCCT 205 127 1684 Antisense
CCATCCTCTCCGATTCAGAATCATA 644 271 1702 Antisense
ATCACTTGAATCACAACACCGAGTC 158 27 1733 Antisense
GAGCCCGCCAGGGTACAGGATGACG 605 385 1777 Antisense
TTTCGTGTACGAACGTCTCCGAGAG 212 671 1836 Antisense
CAGCCTCCGATTGTCGTGGATTTAG 446 201 1873 Antisense
GAACTCCAAGTCTCAATTTCCAGCA 700 343 1980 Antisense
CCAATTTTTGCAGTCACTACCACCA 464 275 2100 Antisense
CACCAGGAATGTTCCCTGATTCTCC 625 201 2129 Antisense
TTCTCCACCAGAATCCGGTTCGGGA 322 687 2148 Antisense
Sequence Source Affymetrix Proprietary Database