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[178268_s_at]

Description C35D6.1 /REP_DB=WormBase Gene ID /WP=CE15681 /TR=O46344 /GB=CAB05144.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 88      4grp_total > 04      6grp_cgc > 69      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 64
1 2 3 4 range
A 8.3
(-3)
A 		7.8
(-3)
A 		10.3
(-2.8)
A 		69.7
(3)
A 		62
B 29.5
(-0.1)
A 		14
(-2.4)
A 		9.7
(-3)
A 		50.5
(3)
A 		41
C 40.5
(0.4)
A 		12.7
(-2.3)
A 		5.3
(-3)
A 		67.5
(3)
A 		62
avr 26.1
(-1) 		11.5
(-2.7) 		8.4
(-3) 		62.6
(3) 		54

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 35
1 2 3 4 5 6 range
A 7.5
(-2.6)
A 		8.4
(-2.4)
A 		6.6
(-2.8)
A 		5.4
(-3)
A 		34.6
(3)
A 		30.2
(2.1)
A 		29
B 14.3
(-0.9)
A 		10
(-1.8)
A 		4
(-3)
A 		23.7
(0.9)
A 		22.8
(0.8)
A 		34
(3)
A 		30
C 4.2
(-3)
A 		14.2
(-1.3)
A 		10.9
(-1.8)
A 		6.3
(-2.6)
A 		12.8
(-1.5)
A 		39
(3)
A 		35
avr 8.7
(-2.7) 		10.9
(-2.2) 		7.2
(-3) 		11.8
(-2) 		23.4
(0.6) 		34.4
(3) 		27
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 12
1 2 3 4 range
A 3
(-2.1)
A 		1.7
(-3)
A 		2.7
(-2.3)
A 		10.2
(3)
A 		9
B 9.4
(2)
A 		3.4
(-2.2)
A 		2.3
(-3)
A 		10.8
(3)
A 		9
C 13.4
(3)
A 		3.7
(-2.1)
A 		1.9
(-3)
A 		11.4
(2)
A 		12
avr 8.6
(1.4) 		2.9
(-2.6) 		2.3
(-3) 		10.8
(3) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 12
1 2 3 4 5 6 range
A 1.4
(-3)
A 		3
(-1.9)
A 		1.4
(-3)
A 		1.4
(-3)
A 		10.4
(3)
A 		7.4
(1)
A 		9
B 5
(-0.9)
A 		3.3
(-1.8)
A 		1
(-3)
A 		6.3
(-0.3)
A 		9.3
(1.3)
A 		12.7
(3)
A 		12
C 1.6
(-2.8)
A 		4.3
(-1.5)
A 		2.8
(-2.2)
A 		1.3
(-3)
A 		4.2
(-1.5)
A 		13.1
(3)
A 		12
avr 2.7
(-2.4) 		3.5
(-1.9) 		1.7
(-3) 		3
(-2.2) 		8
(1) 		11.1
(3) 		9
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 178268_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9055
Sequence Type Exemplar sequence
Representative Public ID C35D6.1 NCBI
Target Description C35D6.1 /REP_DB=WormBase Gene ID /WP=CE15681 /TR=O46344 /GB=CAB05144.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070545(11), NM_070546(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070545 NCBI Caenorhabditis elegans Serpentine Receptor, class H family member (srh-228) (srh-228) mRNA, complete cds. 11/11 None
NM_070546 NCBI Caenorhabditis elegans Serpentine Receptor, class H family member (srh-227) (srh-227) mRNA, complete cds. 11/11 None
SNAP00000023350 ENSEMBL cdna:SNAP chromosome:CEL140:IV:16339626:16341335:1 11/11 None
SNAP00000023351 ENSEMBL cdna:SNAP chromosome:CEL140:IV:16342451:16344160:-1 11/11 None
GENEFINDER00000023358 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:16339875:16341335:1 11/11 None
GENEFINDER00000023360 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:16342451:16343911:-1 11/11 None
C35D6.1 ENSEMBL cdna:known chromosome:CEL140:IV:16339626:16341335:1 gene:C35D6.1 11/11 None
C35D6.2 ENSEMBL cdna:known chromosome:CEL140:IV:16342451:16344160:-1 gene:C35D6.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:16339631-16341341(+) 100.0 100.0
chrIV:16342456-16344166(-) 100.0 100.0
Public Domain and Genome References
Gene Title

Gene Symbol srh-228
srh-227
Ensembl C35D6.1
C35D6.2
Entrez Gene 183232 Entrez gene
183233 Entrez gene
SwissProt O46344 EMBL-EBI
WormBase CE15681 Wormbase
CE15681 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4930 G-protein coupled receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB05145 Hypothetical protein C35D6.2 [Caenorhabditis elegans] emb|CAB05144.1| Hypothetical protein C35D6.1 [Caenorhabditis elegans] ref|NP_502947.1| Serpentine Receptor, class H family member (srh-227) [Caenorhabditis elegans] ref|NP_502946.1| Serpentine Receptor, class H family member (srh-228) [Caenorhabditis elegans] 0.0
blast CAB05145 Hypothetical protein C35D6.2 [Caenorhabditis elegans] emb|CAB05144.1| Hypothetical protein C35D6.1 [Caenorhabditis elegans] ref|NP_502947.1| Serpentine Receptor, class H family member (srh-227) [Caenorhabditis elegans] ref|NP_502946.1| Serpentine Receptor, class H family member (srh-228) [Caenorhabditis elegans] 1.0E-141
blast T33705 hypothetical protein F58E2.6 - Caenorhabditis elegans 1.0E-130
Protein Domains
Database ID Description E-Value
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.0E-126
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.4E-80
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_502946.1 7 22-44,65-87,102-124,137-159,196-218,239-261,271-293
NP_502947.1 7 22-44,65-87,102-124,137-159,196-218,239-261,271-293
C35D6.1 7 22-44,65-87,102-124,137-159,196-218,239-261,271-293
C35D6.2 7 22-44,65-87,102-124,137-159,196-218,239-261,271-293
SNAP00000023350 7 22-44,65-87,102-124,137-159,196-218,239-261,271-293
SNAP00000023351 7 22-44,65-87,102-124,137-159,196-218,239-261,271-293
GENEFINDER00000023358 5 20-42,54-76,113-135,156-178,188-210
GENEFINDER00000023360 5 20-42,54-76,113-135,156-178,188-210
Sequence
Target Sequence 
>C. ELEGANS:178268_S_AT
tcttctactttacggctgtagccacttttgtcccagtttatatcggaatggcaccaggga
ttgagtataagaaagagttcgtcatagagtcccttccttgcctacctgatgcagttcgtg
cccttcctctctaccttgccgttgaaaacaaatggcaatttctgatatggaccgtaggtg
aatcaactctattctgcgtcacttgtcttgcactcttcttacaaatctaccgtgctcttc
gaaaatatggcgaagtccgttccaaacaaacccttgagctccagaaaaggctattcaaag
ctatatttttgcaattggcaattccattttccatcatctctatgccaatgatttactaca
cctatacaccgttttttaatgctattttgaatagtatcatgtttattacagtctcgtccc
atggatttatatcgacaattgttatgcttatcgtgcagaagccgtaccgagagtttattt
taggaggatttgagaagtttttgagacggaaaatgcctcgtgatcttgatttggcgagag
cttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCTTCTACTTTACGGCTGTAGCCAC 127 617 437 Antisense
TAGCCACTTTTGTCCCAGTTTATAT 425 651 455 Antisense
AAAGAGTTCGTCATAGAGTCCCTTC 165 121 508 Antisense
TCTACCGTGCTCTTCGAAAATATGG 299 615 662 Antisense
TCCAAACAAACCCTTGAGCTCCAGA 126 587 697 Antisense
GATTTACTACACCTATACACCGTTT 33 429 786 Antisense
TACACCGTTTTTTAATGCTATTTTG 611 643 801 Antisense
CATGTTTATTACAGTCTCGTCCCAT 240 207 834 Antisense
GTCTCGTCCCATGGATTTATATCGA 370 471 847 Antisense
CTTATCGTGCAGAAGCCGTACCGAG 710 219 883 Antisense
GTGATCTTGATTTGGCGAGAGCTTC 659 483 956 Antisense
Sequence Source Affymetrix Proprietary Database