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[178256_at]

Description T28F3.6 /REP_DB=WormBase Gene ID /WP=CE20116 /TR=Q9XUC2 /GB=CAB05299.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 45      4grp_total > 21      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 145
1 2 3 4 range
A 142.1
(2)
P 		74.7
(-0.9)
A 		25.3
(-3)
A 		165.7
(3)
P 		140
B 105.1
(0.8)
P 		111.2
(1.5)
M 		73.5
(-3)
A 		123.6
(3)
P 		50
C 157.9
(2.3)
P 		100.8
(-0.7)
P 		58.8
(-3)
A 		170
(3)
P 		111
avr 135
(1.9) 		95.6
(-0.4) 		52.5
(-3) 		153.1
(3) 		101

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 104
1 2 3 4 5 6 range
A 105.9
(3)
P 		78.6
(-0.2)
P 		97.1
(2)
P 		79.1
(-0.1)
A 		54.6
(-3)
A 		80.6
(0)
P 		51
B 137.8
(3)
P 		110.9
(1.3)
P 		133.8
(2.7)
P 		56.7
(-2.3)
P 		45.3
(-3)
A 		100.2
(0.6)
P 		93
C 102.6
(2.3)
P 		104
(2.4)
P 		58.2
(-1.1)
P 		78.1
(0.4)
A 		33.8
(-3)
A 		111.2
(3)
P 		77
avr 115.4
(3) 		97.8
(1.5) 		96.4
(1.4) 		71.3
(-0.7) 		44.6
(-3) 		97.3
(1.5) 		71
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 46
1 2 3 4 range
A 51.7
(3)
P 		16.2
(-1.7)
A 		6.6
(-3)
A 		24.3
(-0.6)
P 		45
B 33.5
(3)
P 		27.5
(0.8)
M 		17
(-3)
A 		26.3
(0.4)
P 		17
C 52.3
(3)
P 		29
(-1.4)
P 		20.5
(-3)
A 		28.7
(-1.5)
P 		32
avr 45.8
(3) 		24.2
(-1.2) 		14.7
(-3) 		26.4
(-0.7) 		31

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 37
1 2 3 4 5 6 range
A 19.5
(-1.4)
P 		27.9
(3)
P 		21.1
(-0.6)
P 		20.1
(-1.1)
A 		16.5
(-3)
A 		19.6
(-1.4)
P 		11
B 47.9
(3)
P 		36.9
(1)
P 		32.5
(0.2)
P 		15
(-3)
P 		18.4
(-2.4)
A 		37.4
(1.1)
P 		33
C 38
(3)
P 		31.7
(1.6)
P 		14.8
(-2.1)
P 		15.7
(-1.9)
A 		10.9
(-3)
A 		37.2
(2.8)
P 		27
avr 35.1
(3) 		32.2
(2.1) 		22.8
(-0.7) 		16.9
(-2.5) 		15.3
(-3) 		31.4
(1.9) 		20
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178256_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8733
Sequence Type Exemplar sequence
Representative Public ID T28F3.6 NCBI
Target Description T28F3.6 /REP_DB=WormBase Gene ID /WP=CE20116 /TR=Q9XUC2 /GB=CAB05299.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070672(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070672 NCBI Caenorhabditis elegans T28F3.6 (T28F3.6) mRNA, complete cds. 11/11 None
GENEFINDER00000032173 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:17300812:17301988:-1 11/11 None
T28F3.6 ENSEMBL cdna:known chromosome:CEL140:IV:17300812:17301988:-1 gene:T28F3.6 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000032161 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:17300817-17301994(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T28F3.6
Entrez Gene 189055 Entrez gene
SwissProt Q9XUC2 EMBL-EBI
WormBase CE20116 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB05299 Hypothetical protein T28F3.6 [Caenorhabditis elegans] ref|NP_503073.1| T28F3.6 [Caenorhabditis elegans] 1.0E-134
blast CAE57418 Hypothetical protein CBG00375 [Caenorhabditis briggsae] 1.0E-67
blast NP_001017884 hypothetical protein LOC550583 [Danio rerio] gb|AAH92964.1| Hypothetical protein LOC550583 [Danio rerio] 2.0E-14
Sequence
Target Sequence 
>C. ELEGANS:178256_AT
atttctcgccgattttatggaagatggagacacattgaaaaaaggagaagattccggaga
acgagaaagaaaatttgaattcgagtttacaagtgtctataggccaacaaaaggagtcag
aatacaagaatttgaaacccatgaattttttaccgaacaggagcaaaacgagttaggcgg
aacgagaagactggaggactcagagattcagctttgggatgttagtggagataaaaagta
cgaagactgctggccagcaataaaagaaaatgccgaaggagtcattcttgttgtgaatcc
ggaagagcacaagggctcagaccttcaacaatggttctatgaatttgtagagaaggagaa
tattgatttgagttgtgtcatggtgattctgaacgaacaaggagcaaagaagaccaatca
tgaacagatttctggattcgaaattctgccaaagcttcgcggtgtccaccacgtggctca
tcatttcggctctgaggctatgcaagtcaagatggaagtcaatagcttcatggcatcggt
tttgaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATTTCTCGCCGATTTTATGGAAGAT 562 15 150 Antisense
AGACTGGAGGACTCAGAGATTCAGC 406 69 337 Antisense
GAGATTCAGCTTTGGGATGTTAGTG 639 395 352 Antisense
AAGTACGAAGACTGCTGGCCAGCAA 585 157 385 Antisense
ATGCCGAAGGAGTCATTCTTGTTGT 667 41 419 Antisense
GAGCACAAGGGCTCAGACCTTCAAC 310 389 454 Antisense
GGCTCAGACCTTCAACAATGGTTCT 537 537 463 Antisense
GAGTTGTGTCATGGTGATTCTGAAC 603 397 519 Antisense
AAATTCTGCCAAAGCTTCGCGGTGT 50 117 590 Antisense
CATCATTTCGGCTCTGAGGCTATGC 684 211 628 Antisense
ATAGCTTCATGGCATCGGTTTTGAA 473 21 671 Antisense
Sequence Source Affymetrix Proprietary Database