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[178254_at]

Description E04D5.2 /REP_DB=WormBase Gene ID /WP=CE03123 /TR=Q19055 /GB=CAA91282.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 16      4grp_total > 21      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 336
1 2 3 4 range
A 394
(0.5)
P 		316.5
(-1.1)
P 		223.2
(-3)
A 		520.2
(3)
P 		297
B 499
(3)
P 		297.3
(-0.8)
A 		184.3
(-3)
A 		492.1
(2.9)
P 		315
C 388.6
(0.5)
P 		278.9
(-2)
A 		234.9
(-3)
A 		495.2
(3)
P 		260
avr 427.2
(1.4) 		297.6
(-1.3) 		214.1
(-3) 		502.5
(3) 		288

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 190
1 2 3 4 5 6 range
A 353
(3)
P 		267.4
(0.1)
P 		204.6
(-2.1)
P 		227.6
(-1.3)
A 		176.9
(-3)
A 		210.9
(-1.8)
A 		176
B 321.7
(3)
P 		267.5
(0.8)
P 		180.9
(-2.6)
A 		170.6
(-3)
A 		235.7
(-0.4)
P 		172.9
(-2.9)
P 		151
C 298.7
(3)
P 		266.8
(1.6)
P 		199
(-1.4)
A 		163
(-3)
A 		180
(-2.2)
A 		191.6
(-1.7)
A 		136
avr 324.5
(3) 		267.2
(0.5) 		194.8
(-2.7) 		187.1
(-3) 		197.5
(-2.5) 		191.8
(-2.8) 		137
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 116
1 2 3 4 range
A 143.5
(3)
P 		68.7
(-2.3)
P 		58.3
(-3)
A 		76.2
(-1.7)
P 		85
B 159.1
(3)
P 		73.4
(-1.4)
A 		42.7
(-3)
A 		104.9
(0.2)
P 		116
C 128.6
(3)
P 		80.2
(-3)
A 		81.9
(-2.8)
A 		83.5
(-2.6)
P 		48
avr 143.7
(3) 		74.1
(-2) 		61
(-3) 		88.2
(-1) 		83

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 79
1 2 3 4 5 6 range
A 65.1
(-0.6)
P 		95.1
(3)
P 		44.6
(-3)
P 		57.7
(-1.4)
A 		53.4
(-2)
A 		51.4
(-2.2)
A 		51
B 111.9
(3)
P 		89.1
(1)
P 		43.9
(-3)
A 		45.2
(-2.9)
A 		95.8
(1.6)
P 		64.5
(-1.2)
P 		68
C 110.7
(3)
P 		81.4
(0.7)
P 		50.6
(-1.6)
A 		32.8
(-3)
A 		58.2
(-1)
A 		64.2
(-0.6)
A 		78
avr 95.9
(3) 		88.5
(2.1) 		46.4
(-2.9) 		45.2
(-3) 		69.1
(-0.2) 		60
(-1.2) 		51
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178254_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3616
Sequence Type Exemplar sequence
Representative Public ID E04D5.2 NCBI
Target Description E04D5.2 /REP_DB=WormBase Gene ID /WP=CE03123 /TR=Q19055 /GB=CAA91282.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063840(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063840 NCBI Caenorhabditis elegans E04D5.2 (E04D5.2) mRNA, complete cds. 11/11 None
SNAP00000023766 ENSEMBL cdna:SNAP chromosome:CEL140:II:10419140:10421912:-1 11/11 None
GENEFINDER00000023768 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:10419140:10421382:-1 11/11 None
E04D5.2 ENSEMBL cdna:known chromosome:CEL140:II:10419140:10422239:-1 gene:E04D5.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:10419136-10422236(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl E04D5.2
Entrez Gene 184036 Entrez gene
SwissProt Q19055 EMBL-EBI
WormBase CE03123 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA91282 Hypothetical protein E04D5.2 [Caenorhabditis elegans] ref|NP_496241.1| E04D5.2 [Caenorhabditis elegans] 0.0
blast CAE59684 Hypothetical protein CBG03107 [Caenorhabditis briggsae] 0.0
blast CAA91282 Hypothetical protein E04D5.2 [Caenorhabditis elegans] ref|NP_496241.1| E04D5.2 [Caenorhabditis elegans] 1.0E-177
blast CAE59684 Hypothetical protein CBG03107 [Caenorhabditis briggsae] 1.0E-162
blast CAE59684 Hypothetical protein CBG03107 [Caenorhabditis briggsae] 1.0E-161
blast CAA91282 Hypothetical protein E04D5.2 [Caenorhabditis elegans] ref|NP_496241.1| E04D5.2 [Caenorhabditis elegans] 1.0E-152
blast CAE75484 Hypothetical protein CBG23488 [Caenorhabditis briggsae] 3.0E-48
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_496241.1 7 62-84,105-127,142-161,168-190,222-244,313-335,355-377
E04D5.2 7 62-84,105-127,142-161,168-190,222-244,313-335,355-377
SNAP00000023766 6 24-46,61-80,101-123,155-177,288-310,330-352
GENEFINDER00000023768 6 12-34,54-76,97-119,157-179,290-312,332-354
Sequence
Target Sequence 
>C. ELEGANS:178254_AT
cattagacggaacacctttcgccgccacagaagcatacgaaatatacgatttccatgtta
tcactgcaattcagatgatgtttccgtttgtggtactattgctactgaatctaacaatta
tcaagagattggtagctgagaaaagggaaaatatgtaccctattctccgaggtgctggaa
ctacaacagaagtgaaaaaagcgtcatttgttcagggaaatcttccagaaaactatgtgc
ttctgcaagttgccgctgacgtcatcaaggaatcattgatccacagatcatcccgaagta
agcgatcacagcttcgaaatgcaatttacacgatgttggctattgtcacttcatatttgg
tttgtaatggcgttcatctttttctgacgatccttgagaggttcgatccatcttatcttt
acgaatccacggatcgaatgcagtcgagcacattttatatcgttttgtctgatactgtct
ccatctgctatatggcatcctcggcaattcgtattttcatctacgccaagtgtaatccaa
agttgcgacaggagatcaccgactac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CATTAGACGGAACACCTTTCGCCGC 41 189 623 Antisense
GATTTCCATGTTATCACTGCAATTC 94 429 670 Antisense
TACCCTATTCTCCGAGGTGCTGGAA 601 647 778 Antisense
CTATGTGCTTCTGCAAGTTGCCGCT 390 225 855 Antisense
TTGCCGCTGACGTCATCAAGGAATC 183 697 872 Antisense
CACGATGTTGGCTATTGTCACTTCA 71 201 951 Antisense
ATGGCGTTCATCTTTTTCTGACGAT 671 53 989 Antisense
GAGGTTCGATCCATCTTATCTTTAC 526 403 1020 Antisense
TCGTTTTGTCTGATACTGTCTCCAT 191 605 1082 Antisense
TATGGCATCCTCGGCAATTCGTATT 375 655 1113 Antisense
GTTGCGACAGGAGATCACCGACTAC 86 439 1164 Antisense
Sequence Source Affymetrix Proprietary Database