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[178253_at]

Description F49H6.4 /REP_DB=WormBase Gene ID /WP=CE18714 /TR=Q9XV05 /GB=CAB04439.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 70      4grp_total > 25      6grp_cgc > 95      6grp_cgc_sub > 04      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 34
1 2 3 4 range
A 34.5
(-3)
A 		48.1
(3)
M 		43.6
(1)
A 		41.2
(-0)
A 		14
B 26.2
(-3)
A 		30.5
(-0.3)
A 		34.2
(2.1)
A 		35.6
(3)
A 		9
C 26.6
(-3)
A 		46.2
(0.6)
A 		40.1
(-0.6)
A 		59.7
(3)
M 		33
avr 29.1
(-3) 		41.6
(1.6) 		39.3
(0.7) 		45.5
(3) 		16

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 65
1 2 3 4 5 6 range
A 29.8
(-0.2)
A 		35.6
(0.5)
A 		13.1
(-2.3)
A 		37.4
(0.7)
A 		7
(-3)
A 		56.3
(3)
A 		49
B 43.3
(-0.6)
P 		42.6
(-0.6)
A 		29.9
(-2.2)
A 		72.3
(3)
A 		47.8
(-0)
A 		23.3
(-3)
A 		49
C 25.8
(-1.9)
A 		31.6
(-1.2)
A 		64.7
(3)
A 		43.9
(0.4)
M 		38.1
(-0.4)
A 		17.4
(-3)
A 		47
avr 33
(-2.4) 		36.6
(-1.3) 		35.9
(-1.5) 		51.2
(3) 		31
(-3) 		32.3
(-2.6) 		20
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 8
1 2 3 4 range
A 12.6
(3)
A 		10.4
(1)
M 		11.4
(1.9)
A 		6
(-3)
A 		7
B 8.3
(3)
A 		7.5
(-3)
A 		7.9
(0)
A 		7.6
(-2.3)
A 		1
C 8.8
(-3)
A 		13.3
(2.2)
A 		14
(3)
A 		10.1
(-1.5)
M 		5
avr 9.9
(0.8) 		10.4
(1.7) 		11.1
(3) 		7.9
(-3) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 17
1 2 3 4 5 6 range
A 5.5
(-1.2)
A 		12.6
(2.4)
A 		2.8
(-2.6)
A 		9.5
(0.8)
A 		2.1
(-3)
A 		13.7
(3)
A 		12
B 15.1
(0.9)
P 		14.2
(0.4)
A 		7.3
(-3)
A 		19.1
(2.9)
A 		19.4
(3)
A 		8.7
(-2.3)
A 		12
C 9.6
(-0.9)
A 		9.7
(-0.8)
A 		16.5
(3)
A 		8.8
(-1.3)
M 		12.3
(0.6)
A 		5.8
(-3)
A 		11
avr 10.1
(-1) 		12.2
(2.5) 		8.9
(-3) 		12.5
(3) 		11.3
(1) 		9.4
(-2.2) 		4
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178253_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12782
Sequence Type Exemplar sequence
Representative Public ID F49H6.4 NCBI
Target Description F49H6.4 /REP_DB=WormBase Gene ID /WP=CE18714 /TR=Q9XV05 /GB=CAB04439.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074907(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074907 NCBI Caenorhabditis elegans Serpentine Receptor, class H family member (srh-97) (srh-97) mRNA, complete cds. 11/11 None
GENEFINDER00000000016 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:17016986:17022378:1 11/11 None
F49H6.4 ENSEMBL cdna:known chromosome:CEL140:V:17019665:17022378:1 gene:F49H6.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:17019664-17022378(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol srh-97
Ensembl F49H6.4
Entrez Gene 186072 Entrez gene
SwissProt Q9XV05 EMBL-EBI
WormBase CE18714 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4930 G-protein coupled receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB04439 Hypothetical protein F49H6.4 [Caenorhabditis elegans] ref|NP_507308.1| Serpentine Receptor, class H family member (srh-97) [Caenorhabditis elegans] 0.0
blast CAC35812 Hypothetical protein F21H7.11 [Caenorhabditis elegans] ref|NP_507100.2| Serpentine Receptor, class H family member (srh-95) [Caenorhabditis elegans] 1.0E-131
blast CAB04447 Hypothetical protein F49H6.3 [Caenorhabditis elegans] ref|NP_507307.1| F49H6.3 [Caenorhabditis elegans] 4.0E-89
blast CAB07593 Hypothetical protein F21H7.7 [Caenorhabditis elegans] ref|NP_872212.2| Serpentine Receptor, class H family member (srh-104) [Caenorhabditis elegans] 1.0E-88
Protein Domains
Database ID Description E-Value
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.0E-126
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.0E-126
Pfam IPR009545 EMBL-EBI Protein of unknown function DUF1164 2.2E-122
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_507308.1 7 28-50,85-102,106-128,149-171,217-239,259-281,291-313
F49H6.4 7 28-50,85-102,106-128,149-171,217-239,259-281,291-313
GENEFINDER00000000016 10 7-29,49-71,91-113,128-150,374-391,395-417,438-460,506-528,548-570,580-602
Sequence
Target Sequence 
>C. ELEGANS:178253_AT
gactcctctaccatgcaatagtttttttctacaactgcagctgtcttcttttctttttga
caaatcctgctgatcaagaagctgccaaacttcatgcattaactcgttacccatgtccca
caacggagtttttcgaatttccagttaccatactacttactgatcaaaacgttatccaat
ttataagctttattttccttccatcaatgattttacaaacagctggaaatataatattcc
atgctttttgcgcggtttactatctttatatagctccgtcgaaatcagtttcaaaagaaa
ctcagaagaaccaaaaggcatttttgattggtatcctattacaaacatgtattccattga
tgtttatatcagggccactgatcattttagcaattgcttattcgttcggatattactctc
aagaaatgatgaacttatcaatagtctgcgctgcatttcacggggttgcacaaagtgttg
cagttattacagtgcaccatcactaccgga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACTCCTCTACCATGCAATAGTTTT 209 373 455 Antisense
TCTACAACTGCAGCTGTCTTCTTTT 11 615 482 Antisense
TGCATTAACTCGTTACCCATGTCCC 498 577 549 Antisense
TACCCATGTCCCACAACGGAGTTTT 533 641 562 Antisense
GTTACCATACTACTTACTGATCAAA 262 447 598 Antisense
ATAAGCTTTATTTTCCTTCCATCAA 611 25 637 Antisense
TATTCCATGCTTTTTGCGCGGTTTA 66 665 689 Antisense
AGGGCCACTGATCATTTTAGCAATT 105 59 825 Antisense
ATTCGTTCGGATATTACTCTCAAGA 211 9 854 Antisense
CAATAGTCTGCGCTGCATTTCACGG 657 179 893 Antisense
ATTACAGTGCACCATCACTACCGGA 247 11 940 Antisense
Sequence Source Affymetrix Proprietary Database