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[178247_at]

Description F27D4.2 /REP_DB=WormBase Gene ID /WP=CE05737 /TR=Q93616 /GB=CAB01968.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 82      6grp_total > 80      4grp_total > 25      6grp_high1_sub > 04      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 240
1 2 3 4 range
A 128.6
(-3)
A 		271.8
(3)
A 		247.1
(2)
A 		216.1
(0.7)
A 		143
B 139.5
(-2.3)
A 		280.2
(3)
A 		122.4
(-3)
A 		188.2
(-0.5)
A 		158
C 219.1
(2.2)
M 		40.5
(-3)
A 		195.2
(1.5)
A 		247
(3)
A 		207
avr 162.4
(-3) 		197.5
(0.9) 		188.2
(-0.2) 		217.1
(3) 		55

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 261
1 2 3 4 5 6 range
A 298.6
(0.6)
P 		172.8
(-3)
M 		274.4
(-0.1)
P 		228.4
(-1.4)
A 		384.2
(3)
P 		363.2
(2.4)
P 		211
B 260.9
(-1.8)
P 		285.2
(-1.2)
P 		313.4
(-0.4)
P 		433.8
(3)
P 		219.4
(-3)
P 		301.8
(-0.7)
P 		214
C 285.5
(0)
P 		317.2
(0.9)
P 		199.5
(-2.4)
A 		390.8
(3)
P 		177.2
(-3)
A 		226.5
(-1.6)
P 		214
avr 281.7
(-1.5) 		258.4
(-3) 		262.4
(-2.7) 		351
(3) 		260.3
(-2.9) 		297.2
(-0.5) 		93
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 61
1 2 3 4 range
A 46.8
(-0.2)
A 		59
(2)
A 		64.6
(3)
A 		31.6
(-3)
A 		33
B 44.5
(-0.6)
A 		69.2
(3)
A 		28.4
(-3)
A 		40.1
(-1.3)
A 		41
C 72.5
(3)
M 		11.6
(-3)
A 		68
(2.6)
A 		41.7
(-0)
A 		61
avr 54.6
(3) 		46.6
(0.1) 		53.7
(2.7) 		37.8
(-3) 		17

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 65
1 2 3 4 5 6 range
A 55
(-3)
P 		61.4
(-2.4)
M 		59.8
(-2.5)
P 		57.9
(-2.7)
A 		115.9
(3)
P 		88.4
(0.3)
P 		61
B 90.8
(-0.7)
P 		94.9
(-0.1)
P 		76.1
(-3)
P 		114.9
(3)
P 		89.1
(-1)
P 		112.6
(2.6)
P 		39
C 105.8
(3)
P 		96.8
(2)
P 		50.8
(-3)
A 		78.6
(0)
P 		57.4
(-2.3)
A 		75.8
(-0.3)
P 		55
avr 83.9
(1.3) 		84.4
(1.4) 		62.2
(-3) 		83.8
(1.3) 		87.5
(2) 		92.3
(3) 		30
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178247_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1304
Sequence Type Exemplar sequence
Representative Public ID F27D4.2 NCBI
Target Description F27D4.2 /REP_DB=WormBase Gene ID /WP=CE05737 /TR=Q93616 /GB=CAB01968.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059746
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
F27D4.2.2 ENSEMBL cdna:known chromosome:CEL140:I:7712699:7715176:-1 gene:F27D4.2 A 
SNAP00000008672 ENSEMBL cdna:SNAP chromosome:CEL140:I:7713339:7714720:-1 None
F27D4.2.3 ENSEMBL cdna:known chromosome:CEL140:I:7712699:7715176:-1 gene:F27D4.2 None
F27D4.2.1 ENSEMBL cdna:known chromosome:CEL140:I:7712699:7715183:-1 gene:F27D4.2 A 
F27D4.2.4 ENSEMBL cdna:known chromosome:CEL140:I:7712699:7715183:-1 gene:F27D4.2 None
GENEFINDER00000008683 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:7713986:7715252:-1 None
NM_059746 NCBI Caenorhabditis elegans F27D4.2 (F27D4.2) mRNA, complete cds. None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000008681 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:7713269-7714651(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F27D4.2
Entrez Gene 172536 Entrez gene
SwissProt Q93616 EMBL-EBI
WormBase CE36140 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB01968 Hypothetical protein F27D4.2 [Caenorhabditis elegans] ref|NP_492147.2| F27D4.2 [Caenorhabditis elegans] 1.0E-47
blast CAE57356 Hypothetical protein CBG00300 [Caenorhabditis briggsae] 1.0E-24
Sequence
Target Sequence 
>C. ELEGANS:178247_AT
gagcatcaccacgtgtttctggaaaaggtggagctccacaaaacggtggaatgaatggga
tgtcgatgatgcagcacatgcaaatgcaagcattccaggcacaaatgatgcaagctcaaa
tgtttcagcagatgccgatgatgatgccagggatggttcctggaatgccaccgggaatgg
ctggattgcctggaatgatgccaccgaataatatggcagctgcaatgcaacattttaatg
cgctccttcagaagatattccaaaaacctcgatatctcaattttctatccatttctgtac
aacatattttcataatcttccagtttcagcaaatggccgccgctgcatcggtagcagttt
ctacaccatcaaggaatccatcaacatctggagccgcttctcgaacacgaactccattaa
ccacatcagccacgaattcacctcgacccaccacagaaccaacgattaaagaagaagaac
cacagacaatggaacatgatgtagctgctgctgtggccgcaactactgctactgcaacaa
t

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGCATCACCACGTGTTTCTGGAAA 500 389 398 Antisense
AGGGATGGTTCCTGGAATGCCACCG 400 57 546 Antisense
GGATTGCCTGGAATGATGCCACCGA 643 507 580 Antisense
AACATTTTAATGCGCTCCTTCAGAA 373 137 626 Antisense
ATTTTCTATCCATTTCTGTACAACA 292 15 677 Antisense
TTTTCATAATCTTCCAGTTTCAGCA 256 677 704 Antisense
CGCCGCTGCATCGGTAGCAGTTTCT 476 255 735 Antisense
GAACACGAACTCCATTAACCACATC 421 351 800 Antisense
TTAACCACATCAGCCACGAATTCAC 696 683 814 Antisense
ACATGATGTAGCTGCTGCTGTGGCC 697 105 891 Antisense
CCGCAACTACTGCTACTGCAACAAT 297 263 914 Antisense
Sequence Source Affymetrix Proprietary Database