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[178234_at]

Description F20C5.1 /REP_DB=WormBase Gene ID /WP=CE03238 /TR=Q19637 /GB=CAA92299.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 68      6grp_high1_10_10 > 86      4grp_highest_8_8 > 62      4grp_high_8_8 > 18      6grp_highest_nonorm > 91      6grp_total > 76      4grp_total > 29      6grp_highest_sub > In      6grp_cgc > 58      6grp_high1_sub > In      6grp_cgc_sub > In      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 181
1 2 3 4 range
A 190
(2.3)
P 		178.3
(1.7)
P 		80.7
(-3)
P 		205.4
(3)
P 		125
B 261.2
(3)
P 		167.3
(-1.5)
P 		136.4
(-3)
P 		190.4
(-0.4)
P 		125
C 204.2
(-0.9)
P 		188.8
(-2.5)
P 		184.5
(-3)
P 		241.1
(3)
P 		57
avr 218.5
(3) 		178.1
(0.1) 		133.9
(-3) 		212.3
(2.6) 		85

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 272
1 2 3 4 5 6 range
A 125
(-3)
P 		268.4
(0.4)
P 		206.5
(-1.1)
P 		319
(1.6)
P 		253.3
(0)
P 		378.3
(3)
P 		253
B 198.9
(-1.4)
P 		225.3
(-0.7)
P 		137.9
(-3)
P 		210
(-1.1)
P 		268.9
(0.5)
P 		362.2
(3)
P 		224
C 175.9
(-3)
P 		203.3
(-2.3)
P 		289.8
(0.1)
P 		215.9
(-1.9)
P 		208.9
(-2.1)
P 		396.5
(3)
P 		221
avr 166.6
(-3) 		232.3
(-1.1) 		211.4
(-1.7) 		248.3
(-0.7) 		243.7
(-0.8) 		379
(3) 		212
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 62
1 2 3 4 range
A 69.2
(3)
P 		38.7
(-0.8)
P 		21.1
(-3)
P 		30.1
(-1.9)
P 		48
B 83.3
(3)
P 		41.3
(-1.9)
P 		31.6
(-3)
P 		40.6
(-2)
P 		52
C 67.6
(3)
P 		54.3
(0)
P 		64.3
(2.3)
P 		40.7
(-3)
P 		27
avr 73.4
(3) 		44.8
(-1.7) 		39
(-2.7) 		37.1
(-3) 		36

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 112
1 2 3 4 5 6 range
A 23
(-3)
P 		95.5
(3)
P 		45
(-1.2)
P 		80.9
(1.8)
P 		76.4
(1.4)
P 		92.1
(2.7)
P 		73
B 69.2
(-0.9)
P 		75
(-0.5)
P 		33.5
(-3)
P 		55.6
(-1.7)
P 		109.3
(1.5)
P 		135.1
(3)
P 		102
C 65.2
(-1.5)
P 		62
(-1.8)
P 		73.8
(-1)
P 		43.4
(-3)
P 		67.6
(-1.4)
P 		132.7
(3)
P 		89
avr 52.5
(-2.9) 		77.5
(-0.7) 		50.8
(-3) 		60
(-2.2) 		84.4
(-0.1) 		120
(3) 		69
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178234_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8761
Sequence Type Exemplar sequence
Representative Public ID F20C5.1 NCBI
Target Description F20C5.1 /REP_DB=WormBase Gene ID /WP=CE03238 /TR=Q19637 /GB=CAA92299.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027965(11), NM_001027964(11), NM_001027966(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027965 NCBI Caenorhabditis elegans Poly(ADP-ribose) Metabolism Enzyme family member (pme-3) (pme-3) mRNA, complete cds. 11/11 None
NM_001027964 NCBI Caenorhabditis elegans Poly(ADP-ribose) Metabolism Enzyme family member (pme-3) (pme-3) mRNA, complete cds. 11/11 None
NM_001027966 NCBI Caenorhabditis elegans Poly(ADP-ribose) Metabolism Enzyme family member (pme-3) (pme-3) mRNA, complete cds. 11/11 None
SNAP00000022214 ENSEMBL cdna:SNAP chromosome:CEL140:IV:8748697:8752425:1 11/11 None
GENEFINDER00000022220 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:8746274:8752480:1 11/11 None
F20C5.1a.1 ENSEMBL cdna:known chromosome:CEL140:IV:8746234:8752658:1 gene:F20C5.1 11/11 A 
F20C5.1b ENSEMBL cdna:known chromosome:CEL140:IV:8746236:8752658:1 gene:F20C5.1 11/11 A 
F20C5.1a.3 ENSEMBL cdna:known chromosome:CEL140:IV:8746236:8752658:1 gene:F20C5.1 11/11 None
F20C5.1a.2 ENSEMBL cdna:known chromosome:CEL140:IV:8746274:8752807:1 gene:F20C5.1 11/11 A 
F20C5.1c ENSEMBL cdna:known chromosome:CEL140:IV:8747380:8752425:1 gene:F20C5.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:8746279-8752229(+) 100.0 100.0
Public Domain and Genome References
Ensembl F20C5.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA92299 Hypothetical protein F20C5.1b [Caenorhabditis elegans] ref|NP_001023136.1| Poly(ADP-ribose) Metabolism Enzyme family member (pme-3) [Caenorhabditis elegans] gb|AAO26317.1| poly ADP-ribose metabolism enzyme-3 short form [Caenorhabditis elegans] 0.0
blast CAD89735 Hypothetical protein F20C5.1a [Caenorhabditis elegans] ref|NP_001023135.1| Poly(ADP-ribose) Metabolism Enzyme family member (pme-3) [Caenorhabditis elegans] gb|AAO26316.1| poly ADP-ribose metabolism enzyme-3 long form [Caenorhabditis elegans] sp|Q867X0|PME3_CAEEL Poly(ADP-ribose) glycohydrolase pme-3 (Poly ADP-ribose metabolism enzyme-3) 0.0
blast CAH60763 Hypothetical protein F20C5.1c [Caenorhabditis elegans] ref|NP_001023137.1| Poly(ADP-ribose) Metabolism Enzyme family member (pme-3) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR007724 EMBL-EBI Poly glycohydrolase 1.0E-126
Pfam IPR007724 EMBL-EBI Poly glycohydrolase 1.0E-126
Pfam IPR007724 EMBL-EBI Poly glycohydrolase 1.0E-126
Pfam IPR007724 EMBL-EBI Poly glycohydrolase 1.0E-126
Pfam IPR007724 EMBL-EBI Poly glycohydrolase 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:178234_AT
gcgaacgaacatcttggtggcggagttttaaatcatgggtctgttcaggaggagatccgt
ttcttgatgtgtccagaaatgatggttggaatgttgttgtgcgagaaaatgaaacaactg
gaagcgatttcaattgttggagcttacgttttcagttcttatactggttatggtcatact
ctaaaatgggcagaacttcaaccaaatcattctcgtcagaatacaaacgaatttcgagat
cgttttggacgtcttcgggtagaaactattgcaatcgatgcaattctgttcaaaggatca
aaattagattgtcagacggagcagttaaacaaagcaaatatcattagggaaatgaagaaa
gcatctatcggattcatgagccagggaccgaaattcacaaatattccaattgttactgga
tggtggggatgtggagcatttaatggggacaagccactgaagttcataatccaagtaatt
gctgccggagtcgctgatcgtccacttcatttctgttcatttggagaacccgagcttgcc
g

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCGAACGAACATCTTGGTGGCGGAG 443 287 1573 Antisense
GGGTCTGTTCAGGAGGAGATCCGTT 625 497 1609 Antisense
GAGATCCGTTTCTTGATGTGTCCAG 274 397 1624 Antisense
GTTGGAGCTTACGTTTTCAGTTCTT 497 435 1708 Antisense
ACTTCAACCAAATCATTCTCGTCAG 233 103 1767 Antisense
GAGATCGTTTTGGACGTCTTCGGGT 570 395 1808 Antisense
GCAATCGATGCAATTCTGTTCAAAG 304 321 1843 Antisense
CTATCGGATTCATGAGCCAGGGACC 676 223 1937 Antisense
CAAGTAATTGCTGCCGGAGTCGCTG 200 183 2044 Antisense
GTCCACTTCATTTCTGTTCATTTGG 245 477 2072 Antisense
TCATTTGGAGAACCCGAGCTTGCCG 296 619 2089 Antisense
Sequence Source Affymetrix Proprietary Database