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[178233_at]

Description F49H6.5 /REP_DB=WormBase Gene ID /WP=CE18715 /TR=Q9XV04 /GB=CAB04440.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 96      4grp_total > 03      6grp_total_sub > Increase      6grp_rule_based > S5      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 263
1 2 3 4 range
A 39.6
(-1.7)
A 		2.1
(-3)
A 		4.6
(-2.9)
A 		175
(3)
P 		173
B 31.5
(-2.4)
A 		6.9
(-2.9)
A 		3.6
(-3)
A 		261.7
(3)
P 		258
C 41.2
(-2.1)
A 		3
(-3)
A 		3.9
(-3)
A 		264.9
(3)
P 		262
avr 37.4
(-2.1) 		4
(-3) 		4
(-3) 		233.9
(3) 		230

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 84
1 2 3 4 5 6 range
A 11
(-3)
A 		20.8
(-2)
A 		37.5
(-0.2)
A 		35.2
(-0.4)
A 		67.8
(3)
A 		56.1
(1.8)
A 		57
B 11.5
(-2.7)
A 		42.4
(-0.4)
A 		7.8
(-3)
A 		75.6
(2)
A 		89.2
(3)
A 		55.4
(0.5)
A 		81
C 5.4
(-3)
A 		5.1
(-3)
A 		49.8
(0.5)
A 		6.9
(-2.9)
A 		59.8
(1.3)
A 		81
(3)
A 		76
avr 9.3
(-3) 		22.8
(-1.7) 		31.7
(-0.9) 		39.2
(-0.2) 		72.3
(3) 		64.2
(2.2) 		63
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 55
1 2 3 4 range
A 14.4
(0.3)
A 		0.5
(-3)
A 		1.2
(-2.8)
A 		25.6
(3)
P 		25
B 10
(-2)
A 		1.7
(-2.9)
A 		0.8
(-3)
A 		55.8
(3)
P 		55
C 13.6
(-1.3)
A 		0.9
(-3)
A 		1.4
(-2.9)
A 		44.7
(3)
P 		44
avr 12.7
(-1.3) 		1
(-3) 		1.1
(-3) 		42
(3) 		41

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 35
1 2 3 4 5 6 range
A 2
(-3)
A 		7.4
(-1.2)
A 		8.2
(-1)
A 		8.9
(-0.8)
A 		20.5
(3)
A 		13.7
(0.8)
A 		19
B 4
(-2.6)
A 		14.1
(-0.9)
A 		1.9
(-3)
A 		20
(0.2)
A 		36.2
(3)
A 		20.7
(0.3)
A 		34
C 2
(-2.9)
A 		1.6
(-3)
A 		12.7
(-0.4)
A 		1.4
(-3)
A 		19.3
(1.2)
A 		27.1
(3)
A 		26
avr 2.7
(-3) 		7.7
(-1.7) 		7.6
(-1.7) 		10.1
(-1) 		25.3
(3) 		20.5
(1.7) 		23
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178233_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13108
Sequence Type Exemplar sequence
Representative Public ID F49H6.5 NCBI
Target Description F49H6.5 /REP_DB=WormBase Gene ID /WP=CE18715 /TR=Q9XV04 /GB=CAB04440.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074910(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074910 NCBI Caenorhabditis elegans F49H6.5 (F49H6.5) mRNA, complete cds. 11/11 None
SNAP00000000010 ENSEMBL cdna:SNAP chromosome:CEL140:V:17026053:17027925:1 9/11 None
GENEFINDER00000000018 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:17026053:17027925:1 11/11 None
F49H6.5 ENSEMBL cdna:known chromosome:CEL140:V:17026053:17027925:1 gene:F49H6.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:17026052-17027925(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F49H6.5
Entrez Gene 186073 Entrez gene
SwissProt Q9XV04 EMBL-EBI
WormBase CE18715 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:15850_AT molybdopterin biosynthesis protein, putative / molybdenum cofactor biosynthesis enzyme, putative at
ATGENOME1:20406_G_AT molybdopterin biosynthesis protein, putative / molybdenum cofactor biosynthesis enzyme, putative at
ATGENOME1:20405_AT molybdopterin biosynthesis protein, putative / molybdenum cofactor biosynthesis enzyme, putative at
ATH1-121501:263472_AT molybdopterin biosynthesis protein, putative / molybdenum cofactor biosynthesis enzyme, putative at
RG-U34B:RC_AA957434_S_AT Molybdenum cofactor synthesis 1 (predicted) rn
RG-U34B:RC_AA946380_AT Molybdenum cofactor synthesis 1 (predicted) rn
RAE230A:1376093_AT Molybdenum cofactor synthesis 1 (predicted) rn
RAT230_2:1376093_AT Molybdenum cofactor synthesis 1 (predicted) rn
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB04440 Hypothetical protein F49H6.5 [Caenorhabditis elegans] ref|NP_507311.1| F49H6.5 [Caenorhabditis elegans] 0.0
blast CAC42302 Hypothetical protein F49E2.1a [Caenorhabditis elegans] ref|NP_509611.3| F49E2.1a [Caenorhabditis elegans] 0.0
blast CAC42301 Hypothetical protein F49E2.1b [Caenorhabditis elegans] ref|NP_509612.2| F49E2.1b [Caenorhabditis elegans] sp|Q20624|YL71_CAEEL Hypothetical protein F49E2.1 in chromosome X 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR010505 EMBL-EBI Molybdenum cofactor synthesis C 2.7E-49
Pfam IPR007197 EMBL-EBI Radical SAM 7.0E-41
Pfam IPR010505 EMBL-EBI Molybdenum cofactor synthesis C 2.7E-49
Pfam IPR007197 EMBL-EBI Radical SAM 1.1E-22
Sequence
Target Sequence 
>C. ELEGANS:178233_AT
atcagcattgattcgcttgatcgtgaaaaatttgctaagatgacaagacgggatggattt
gacaaagtttggaaggcaattgaactagcgagaggttattttccaaaagttaaactgaac
gttgttgttcttaaacaccaaaatgagaacgaaattgttgattttgtgaatctaactaaa
ggtcgtaaccttgacatccgattcatagagttcatgccttttggcggtaacgagttcaaa
aatgacagtttcatgagctatcgagaaatgctgaacttaattgttgataaatatggggat
gccgtcatacggttatccgacttacctaatgacacaactaaagcttataagattgacgga
ttccaaggacaatttgggtttatcacctcgatgtcagatcacttttgtaacacttgcaat
aggctaagaataactgctgatggaaatttgaaagtatgcctccatggaaattccgaagta
agtctacgtgatagaattcgttgtggtgactctgacgcaaagctttccgaggtcattcaa
acagccgtggacaacaaaaaagctagacatgccggaatggacgctctgaaaaacttgcca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATCAGCATTGATTCGCTTGATCGTG 211 27 553 Antisense
GAACTAGCGAGAGGTTATTTTCCAA 240 349 634 Antisense
AGGTCGTAACCTTGACATCCGATTC 103 53 732 Antisense
ATGCCTTTTGGCGGTAACGAGTTCA 394 39 766 Antisense
ATGACAGTTTCATGAGCTATCGAGA 87 45 794 Antisense
GGATGCCGTCATACGGTTATCCGAC 506 513 849 Antisense
GTTATCCGACTTACCTAATGACACA 541 447 864 Antisense
ATTCGTTGTGGTGACTCTGACGCAA 536 9 1048 Antisense
TCTGACGCAAAGCTTTCCGAGGTCA 405 609 1063 Antisense
CCGAGGTCATTCAAACAGCCGTGGA 165 263 1079 Antisense
AATGGACGCTCTGAAAAACTTGCCA 501 159 1128 Antisense
Sequence Source Affymetrix Proprietary Database