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[178230_s_at]

Description R144.4 /REP_DB=WormBase Gene ID /WP=CE25091 /TR=Q09999 /GB=AAK21458.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 94      6grp_high1_10_10 > 48      4grp_high_8_8 > 45      6grp_highest_nonorm > 83      6grp_total > 38      4grp_total > 43      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 144
1 2 3 4 range
A 497.1
(0)
P 		568.4
(3)
A 		459.9
(-1.5)
P 		424.2
(-3)
P 		144
B 437.5
(-3)
P 		506.7
(1.6)
P 		528.6
(3)
P 		462.5
(-1.4)
P 		91
C 488.9
(-0.4)
P 		501.2
(0.9)
A 		462.9
(-3)
P 		522.4
(3)
P 		60
avr 474.5
(-2.5) 		525.4
(3) 		483.8
(-1.5) 		469.7
(-3) 		56

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 298
1 2 3 4 5 6 range
A 481.1
(0.5)
P 		481.4
(0.5)
P 		340.8
(-3)
P 		418.8
(-1)
P 		483.8
(0.6)
P 		579.1
(3)
P 		238
B 453.9
(2.5)
P 		465.6
(3)
P 		395.4
(-0.2)
P 		388.3
(-0.5)
P 		332.8
(-3)
P 		419.6
(0.9)
P 		133
C 524.7
(3)
P 		436.8
(0.8)
P 		392.9
(-0.3)
P 		281.5
(-3)
P 		425.2
(0.5)
P 		449.4
(1.1)
P 		243
avr 486.6
(3) 		461.3
(1.8) 		376.4
(-2.3) 		362.9
(-3) 		413.9
(-0.5) 		482.7
(2.8) 		124
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 119
1 2 3 4 range
A 181
(3)
P 		123.4
(0.1)
A 		120.2
(-0.1)
P 		62.1
(-3)
P 		119
B 139.5
(3)
P 		125.1
(0.9)
P 		122.5
(0.5)
P 		98.6
(-3)
P 		41
C 161.8
(3)
P 		144.1
(1.6)
A 		161.3
(3)
P 		88.1
(-3)
P 		74
avr 160.8
(3) 		130.9
(0.7) 		134.7
(1) 		82.9
(-3) 		78

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 138
1 2 3 4 5 6 range
A 88.7
(-2.1)
P 		171.2
(3)
P 		74.2
(-3)
P 		106.2
(-1)
P 		146
(1.4)
P 		141
(1.1)
P 		97
B 157.9
(3)
P 		155
(2.7)
P 		96
(-3)
P 		102.8
(-2.3)
P 		135.2
(0.8)
P 		156.5
(2.9)
P 		62
C 194.5
(3)
P 		133.3
(0.3)
P 		100
(-1.1)
P 		56.6
(-3)
P 		137.6
(0.5)
P 		150.5
(1.1)
P 		138
avr 147
(2.4) 		153.2
(3) 		90.1
(-2.9) 		88.5
(-3) 		139.6
(1.7) 		149.3
(2.6) 		65
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 178230_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7733
Sequence Type Exemplar sequence
Representative Public ID R144.4 NCBI
Target Description R144.4 /REP_DB=WormBase Gene ID /WP=CE25091 /TR=Q09999 /GB=AAK21458.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171863(10), NM_171109(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171863 NCBI Caenorhabditis elegans R144.4a (R144.4) mRNA, complete cds. 10/11 None
NM_171109 NCBI Caenorhabditis elegans R144.4b (R144.4) mRNA, complete cds. 10/11 None
SNAP00000019425 ENSEMBL cdna:SNAP chromosome:CEL140:III:5018609:5020769:1 10/11 None
GENEFINDER00000019433 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:5018609:5020769:1 10/11 None
R144.4b ENSEMBL cdna:known chromosome:CEL140:III:5018609:5020769:1 gene:R144.4 10/11 None
R144.4a ENSEMBL cdna:known chromosome:CEL140:III:5018609:5021091:1 gene:R144.4 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:5018607-5020768(+) 96.37 100.0
Public Domain and Genome References
Ensembl R144.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_741122 R144.4b [Caenorhabditis elegans] gb|AAP82645.1| Hypothetical protein R144.4b [Caenorhabditis elegans] 8.0E-73
blast NP_741123 R144.4a [Caenorhabditis elegans] gb|AAP82644.1| Hypothetical protein R144.4a [Caenorhabditis elegans] 4.0E-71
blast NP_741123 R144.4a [Caenorhabditis elegans] gb|AAP82644.1| Hypothetical protein R144.4a [Caenorhabditis elegans] 5.0E-70
blast NP_741122 R144.4b [Caenorhabditis elegans] gb|AAP82645.1| Hypothetical protein R144.4b [Caenorhabditis elegans] 8.0E-70
Protein Domains
Database ID Description E-Value
Pfam IPR003124 EMBL-EBI Actin-binding WH2 4.3E-7
Sequence
Target Sequence 
>C. ELEGANS:178230_S_AT
gaacgatgagacctctacgaccagcaaatgatgtgaaaccgacaatgtatcgacgaagtg
gaagttcagaggatataccgcaagcgacgtcggcaaccagactggcacgtccattagctc
ctccgccagctcgcccagctgcaccaccaccgccagtaccaacgccgtccatagcacaaa
tgtccagcagattccagtcgccagcgatatcacggactcttgatgatgctcccccaccac
ctccacgaatcgcttcaaaaacatcagttgctccaacatcatctcgggctccacttcccc
cgtcttcttcatttgcaccttcaatttcagcatctccagcacctgccccacctccacctc
caccgcaggctgcttcaccgtatcctggaatgaaatcaactgcgactgtctcagcgaacg
ttccatttcatccacttaacaggtattttttaaattcatttttttttcttcgcaatcttt
taaattataggttccactttctaccgct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAACGATGAGACCTCTACGACCAGC 36 349 584 Antisense
TATACCGCAAGCGACGTCGGCAACC 340 659 657 Antisense
CAACCAGACTGGCACGTCCATTAGC 509 185 677 Antisense
GCCGTCCATAGCACAAATGTCCAGC 295 283 747 Antisense
CAGTCGCCAGCGATATCACGGACTC 481 207 778 Antisense
CACCTCCACGAATCGCTTCAAAAAC 595 199 821 Antisense
TGCACCTTCAATTTCAGCATCTCCA 612 577 897 Antisense
CTGCTTCACCGTATCCTGGAATGAA 27 239 953 Antisense
AACTGCGACTGTCTCAGCGAACGTT 454 141 981 Antisense
CGTTCCATTTCATCCACTTAACAGG 275 247 1002 Antisense
ATTATAGGTTCCACTTTCTACCGCT 28 3 1067 Antisense
Sequence Source Affymetrix Proprietary Database