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[178225_at]

Description F28C6.9 /REP_DB=WormBase Gene ID /WP=CE03280 /TR=Q19867 /GB=CAA92673.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 05      4grp_total > 45      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 111
1 2 3 4 range
A 90.1
(3)
P 		79.9
(2.3)
A 		30.8
(-1.3)
A 		7.4
(-3)
A 		83
B 118.7
(3)
P 		66.9
(-0.7)
A 		38.4
(-2.7)
A 		34.6
(-3)
A 		84
C 92.9
(3)
P 		61.8
(-1.5)
A 		51.1
(-3)
A 		59.3
(-1.8)
A 		42
avr 100.6
(3) 		69.5
(0.2) 		40.1
(-2.4) 		33.8
(-3) 		67

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 86
1 2 3 4 5 6 range
A 31.7
(-3)
A 		45
(-0.5)
A 		63.4
(3)
P 		37.6
(-1.9)
A 		46.5
(-0.2)
A 		34.4
(-2.5)
A 		32
B 57.2
(3)
A 		46.7
(1.2)
A 		35.7
(-0.6)
A 		21.6
(-3)
A 		45.6
(1)
A 		29.5
(-1.7)
A 		36
C 90.7
(3)
A 		60.9
(0.9)
A 		16.5
(-2.2)
A 		19.5
(-2)
A 		43.8
(-0.3)
A 		5.2
(-3)
A 		86
avr 59.9
(3) 		50.9
(1.5) 		38.5
(-0.5) 		26.2
(-2.5) 		45.3
(0.6) 		23
(-3) 		37
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 37
1 2 3 4 range
A 32.8
(3)
P 		17.4
(0.1)
A 		8
(-1.7)
A 		1.1
(-3)
A 		32
B 37.9
(3)
P 		16.5
(-1.2)
A 		8.9
(-2.7)
A 		7.4
(-3)
A 		31
C 30.7
(3)
P 		17.8
(-0.7)
A 		17.8
(-0.7)
A 		10
(-3)
A 		21
avr 33.8
(3) 		17.2
(-0.6) 		11.6
(-1.8) 		6.2
(-3) 		28

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 32
1 2 3 4 5 6 range
A 5.8
(-3)
A 		16
(3)
A 		13.8
(1.7)
P 		9.5
(-0.8)
A 		14
(1.8)
A 		8.4
(-1.5)
A 		10
B 19.9
(3)
A 		15.6
(1.2)
A 		8.7
(-1.7)
A 		5.7
(-3)
A 		18.5
(2.4)
A 		11
(-0.8)
A 		14
C 33.6
(3)
A 		18.6
(0.2)
A 		4.2
(-2.5)
A 		3.9
(-2.6)
A 		14.2
(-0.6)
A 		1.7
(-3)
A 		32
avr 19.8
(3) 		16.7
(1.6) 		8.9
(-1.9) 		6.4
(-3) 		15.6
(1.1) 		7
(-2.7) 		13
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178225_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3507
Sequence Type Exemplar sequence
Representative Public ID F28C6.9 NCBI
Target Description F28C6.9 /REP_DB=WormBase Gene ID /WP=CE03280 /TR=Q19867 /GB=CAA92673.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063427(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063427 NCBI Caenorhabditis elegans F28C6.9 (F28C6.9) mRNA, complete cds. 11/11 None
SNAP00000038098 ENSEMBL cdna:SNAP chromosome:CEL140:II:8608798:8610032:-1 11/11 None
GENEFINDER00000038105 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:8608798:8610542:-1 11/11 None
F28C6.9 ENSEMBL cdna:known chromosome:CEL140:II:8608798:8610598:-1 gene:F28C6.9 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:8608794-8610595(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F28C6.9
Entrez Gene 185049 Entrez gene
SwissProt Q19867 EMBL-EBI
WormBase CE03280 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA92673 Hypothetical protein F28C6.9 [Caenorhabditis elegans] ref|NP_495828.1| F28C6.9 [Caenorhabditis elegans] 0.0
blast CAA92673 Hypothetical protein F28C6.9 [Caenorhabditis elegans] ref|NP_495828.1| F28C6.9 [Caenorhabditis elegans] 1.0E-139
blast CAE57559 Hypothetical protein CBG00537 [Caenorhabditis briggsae] 1.0E-99
blast CAE57559 Hypothetical protein CBG00537 [Caenorhabditis briggsae] 7.0E-95
blast CAE57561 Hypothetical protein CBG00539 [Caenorhabditis briggsae] 1.0E-62
blast CAE57561 Hypothetical protein CBG00539 [Caenorhabditis briggsae] 4.0E-47
Sequence
Target Sequence 
>C. ELEGANS:178225_AT
gagcaaacatgctttcactgatgcaattggtcaatacatctggataacaattgacaaacc
cattccattatcacaaaatccacacggacaagttgaaattcggaagctaacagttttagg
gtacccattgtcagatgagatggtacggcaaatggagccggaaatagaggaaatccacgc
ggatgagccggtaaaacaggtgcctgaacacaacagcagaccatcatcgaaaagaaatgt
taaagtggaaagacatagctatgaaggtgacgggatgtcaatgaaagaagaaacgaatct
aagtggagatccacttacatcagttcgtacaattcgaagagttttggaacaaaaaatgga
aaaggcaaatttcgatggaaagacaattcaagcaactgtttgtcttcgggcaattcaacg
aattgacgagtatgaagcaaggattgaagatttggcaactagaagaagtaaagctttaga
agttggggatctgaatatggccgaacgtcatcgactggcaatgattgattgtagagatac
agtattccgagcagttcacgttgatttgctgttggat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGCAAACATGCTTTCACTGATGCA 684 389 423 Antisense
ACAAACCCATTCCATTATCACAAAA 632 111 476 Antisense
GAGGAAATCCACGCGGATGAGCCGG 273 403 589 Antisense
GATGAGCCGGTAAAACAGGTGCCTG 527 411 604 Antisense
GAACACAACAGCAGACCATCATCGA 207 353 628 Antisense
GAGATCCACTTACATCAGTTCGTAC 180 397 728 Antisense
GCAACTGTTTGTCTTCGGGCAATTC 20 321 814 Antisense
GGGGATCTGAATATGGCCGAACGTC 637 495 907 Antisense
TGGCCGAACGTCATCGACTGGCAAT 314 543 920 Antisense
AGAGATACAGTATTCCGAGCAGTTC 520 67 955 Antisense
AGTTCACGTTGATTTGCTGTTGGAT 465 63 975 Antisense
Sequence Source Affymetrix Proprietary Database