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[178224_at]

Description F53A2.1 /REP_DB=WormBase Gene ID /WP=CE16093 /TR=O45546 /GB=CAB04449.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 56      4grp_total > 02      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 78
1 2 3 4 range
A 3.6
(-2.8)
A 		1.5
(-3)
A 		1.2
(-3)
A 		79.2
(3)
A 		78
B 5.4
(-3)
A 		6.2
(1)
A 		6.6
(3)
A 		5.6
(-2)
A 		1
C 2.7
(-3)
A 		4.3
(-1.9)
A 		5.5
(-1.1)
A 		11.6
(3)
A 		9
avr 3.9
(-3) 		4
(-3) 		4.4
(-2.9) 		32.1
(3) 		28

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 23
1 2 3 4 5 6 range
A 1.9
(-1.5)
A 		1.2
(-2.4)
A 		5.4
(3)
A 		3.4
(0.4)
A 		0.7
(-3)
A 		2.3
(-1)
A 		5
B 4
(-2.2)
A 		23.7
(3)
A 		3.4
(-2.4)
A 		4.1
(-2.2)
A 		1
(-3)
A 		11.9
(-0.1)
A 		23
C 3.3
(-3)
A 		5.2
(-2.3)
A 		4.8
(-2.4)
A 		4.8
(-2.4)
A 		5.5
(-2.2)
A 		19.1
(3)
A 		16
avr 3.1
(-2.5) 		10
(2.2) 		4.5
(-1.6) 		4.1
(-1.8) 		2.4
(-3) 		11.1
(3) 		9
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 11
1 2 3 4 range
A 1.3
(-2.5)
A 		0.3
(-3)
A 		0.3
(-3)
A 		11.6
(3)
A 		11
B 1.7
(3)
A 		1.5
(0.6)
A 		1.5
(0.6)
A 		1.2
(-3)
A 		1
C 0.9
(-3)
A 		1.3
(-0.8)
A 		1.9
(2.5)
A 		2
(3)
A 		1
avr 1.3
(-2.5) 		1
(-3) 		1.2
(-2.7) 		4.9
(3) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 8
1 2 3 4 5 6 range
A 0.3
(-2.4)
A 		0.4
(-1.8)
A 		1.2
(3)
A 		0.9
(1.2)
A 		0.2
(-3)
A 		0.6
(-0.6)
A 		1
B 1.4
(-2.2)
A 		7.9
(3)
A 		0.8
(-2.7)
A 		1.1
(-2.4)
A 		0.4
(-3)
A 		4.4
(0.2)
A 		8
C 1.2
(-2.8)
A 		1.6
(-2.3)
A 		1.2
(-2.8)
A 		1
(-3)
A 		1.8
(-2.1)
A 		6.4
(3)
A 		5
avr 1
(-2.6) 		3.3
(2) 		1.1
(-2.4) 		1
(-2.6) 		0.8
(-3) 		3.8
(3) 		3
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178224_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6795
Sequence Type Exemplar sequence
Representative Public ID F53A2.1 NCBI
Target Description F53A2.1 /REP_DB=WormBase Gene ID /WP=CE16093 /TR=O45546 /GB=CAB04449.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067345(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067345 NCBI Caenorhabditis elegans F53A2.1 (F53A2.1) mRNA, complete cds. 11/11 None
GENEFINDER00000020120 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:13318591:13321088:-1 11/11 None
SNAP00000020104 ENSEMBL cdna:SNAP chromosome:CEL140:III:13318781:13320411:-1 11/11 None
F53A2.1 ENSEMBL cdna:known chromosome:CEL140:III:13318781:13324355:-1 gene:F53A2.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:13318776-13324351(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F53A2.1
Entrez Gene 186137 Entrez gene
SwissProt O45546 EMBL-EBI
WormBase CE28830 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04449 Hypothetical protein F53A2.1 [Caenorhabditis elegans] ref|NP_499746.1| F53A2.1 [Caenorhabditis elegans] 0.0
blast CAE56551 Hypothetical protein CBG24284 [Caenorhabditis briggsae] 2.0E-99
blast AAK68494 Hypothetical protein Y48G1BM.2 [Caenorhabditis elegans] ref|NP_490704.1| Y48G1BM.2 [Caenorhabditis elegans] 7.0E-69
blast AAK68496 Hypothetical protein Y48G1BM.4 [Caenorhabditis elegans] ref|NP_490700.1| Y48G1BM.4 [Caenorhabditis elegans] 1.0E-66
blast AAK68494 Hypothetical protein Y48G1BM.2 [Caenorhabditis elegans] ref|NP_490704.1| Y48G1BM.2 [Caenorhabditis elegans] 1.0E-66
Protein Domains
Database ID Description E-Value
Pfam IPR009497 EMBL-EBI Protein of unknown function DUF1114 1.0E-126
Pfam IPR009497 EMBL-EBI Protein of unknown function DUF1114 1.0E-126
Pfam IPR009497 EMBL-EBI Protein of unknown function DUF1114 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_499746.1 3 25-47,62-79,92-111
F53A2.1 3 25-47,62-79,92-111
Sequence
Target Sequence 
>C. ELEGANS:178224_AT
acacatcaggctgtttcgaactggaatgcaaagaatgttaaccttaattttcgattcgga
gcgcacaatgttatggcagaaaaatttcacgagtgcccagaaaatttcaagaatccagtg
aatattcagtttgttgcaccatggaacattgaaccagtttctgctaatttattggaaagt
gtggaaattaagttacaatacacggatgatattctacttaccattgaagatcagttggat
tatgactattattcaccatatgataatataattgcaaatgttcaaagggccttcccaagc
aggaatatgattgtgaaactttctgagacgaatgttctaattgctcctgaaaatatgcac
aaagtctttgcaaagtttattaggttgactgagcaaaatcaacagcgaaatggaagtgtc
acatttaaattgtactatgaagaagacgatagaattccgataaaagtcccacacattttc
aattcaagcgcacgtctcgtaggagaacaacctgaacattgttggtatctcgatgaaatt
ctcaatattccaaaagaaaccattaaaagcccttttggatgtgtaact

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACACATCAGGCTGTTTCGAACTGGA 241 111 2035 Antisense
ATTTTCGATTCGGAGCGCACAATGT 104 13 2081 Antisense
GAATATTCAGTTTGTTGCACCATGG 346 325 2154 Antisense
TGGAACATTGAACCAGTTTCTGCTA 407 547 2176 Antisense
TACACGGATGATATTCTACTTACCA 450 643 2233 Antisense
TGTTCAAAGGGCCTTCCCAAGCAGG 126 557 2313 Antisense
AGACGAATGTTCTAATTGCTCCTGA 592 69 2360 Antisense
GAATTCCGATAAAAGTCCCACACAT 523 323 2486 Antisense
AAAGTCCCACACATTTTCAATTCAA 33 119 2497 Antisense
TCAATTCAAGCGCACGTCTCGTAGG 465 631 2513 Antisense
TAAAAGCCCTTTTGGATGTGTAACT 232 627 2598 Antisense
Sequence Source Affymetrix Proprietary Database