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[178222_at]

Description B0464.8 /REP_DB=WormBase Gene ID /WP=CE20460 /TR=Q9XVX8 /GB=CAA79545.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 77      6grp_high1_10_10 > 77      4grp_highest_8_8 > 63      4grp_high_8_8 > 19      6grp_highest_nonorm > 94      6grp_total > 77      4grp_total > 29      6grp_highest_sub > Da      6grp_cgc > 49      6grp_high1_sub > Da      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 152
1 2 3 4 range
A 386.4
(0.9)
P 		428.8
(3)
P 		304.8
(-3)
P 		375.6
(0.4)
P 		124
B 399
(1.3)
P 		388.9
(0.9)
P 		289.8
(-3)
P 		441.3
(3)
P 		152
C 392.2
(1.7)
P 		304.8
(-2.5)
P 		294
(-3)
P 		420.2
(3)
P 		126
avr 392.5
(2) 		374.2
(1) 		296.2
(-3) 		412.4
(3) 		116

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 768
1 2 3 4 5 6 range
A 420.7
(-2.5)
P 		365.8
(-3)
P 		385.1
(-2.8)
P 		416.4
(-2.5)
P 		457.5
(-2.1)
P 		965.7
(3)
P 		600
B 363.8
(-2.2)
P 		275.7
(-3)
P 		341.6
(-2.4)
P 		298.5
(-2.8)
P 		540.4
(-0.6)
P 		927.1
(3)
P 		651
C 412.5
(-2.3)
P 		326.4
(-3)
P 		472.1
(-1.8)
P 		374.8
(-2.6)
P 		480.6
(-1.7)
P 		1043.7
(3)
P 		717
avr 399
(-2.3) 		322.6
(-3) 		399.6
(-2.3) 		363.2
(-2.6) 		492.8
(-1.4) 		978.8
(3) 		656
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 86
1 2 3 4 range
A 140.7
(3)
P 		93.1
(-0.3)
P 		79.7
(-1.3)
P 		55
(-3)
P 		86
B 127.2
(3)
P 		96
(-0.1)
P 		67.2
(-3)
P 		94
(-0.3)
P 		60
C 129.8
(3)
P 		87.6
(-1.3)
P 		102.5
(0.2)
P 		70.9
(-3)
P 		59
avr 132.6
(3) 		92.2
(-1.1) 		83.1
(-2) 		73.3
(-3) 		59

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 274
1 2 3 4 5 6 range
A 77.5
(-3)
P 		130.1
(-1)
P 		83.9
(-2.8)
P 		105.6
(-1.9)
P 		138.1
(-0.7)
P 		235.1
(3)
P 		158
B 126.6
(-1.9)
P 		91.8
(-2.7)
P 		83
(-2.9)
P 		79.1
(-3)
P 		219.6
(0.2)
P 		345.7
(3)
P 		267
C 152.9
(-1.3)
P 		99.6
(-2.5)
P 		120.2
(-2)
P 		75.4
(-3)
P 		155.5
(-1.2)
P 		349.4
(3)
P 		274
avr 119
(-2.1) 		107.2
(-2.4) 		95.7
(-2.8) 		86.7
(-3) 		171.1
(-0.7) 		310.1
(3) 		223
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178222_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6784
Sequence Type Exemplar sequence
Representative Public ID B0464.8 NCBI
Target Description B0464.8 /REP_DB=WormBase Gene ID /WP=CE20460 /TR=Q9XVX8 /GB=CAA79545.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066685(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066685 NCBI Caenorhabditis elegans B0464.8 (B0464.8) mRNA, complete cds. 11/11 None
SNAP00000014947 ENSEMBL cdna:SNAP chromosome:CEL140:III:9468053:9469719:1 11/11 None
GENEFINDER00000014957 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:9468053:9469719:1 11/11 None
B0464.8 ENSEMBL cdna:known chromosome:CEL140:III:9468053:9469730:1 gene:B0464.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:9468048-9469715(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol tag-342
Ensembl B0464.8
Entrez Gene 176331 Entrez gene
SwissProt Q9XVX8 EMBL-EBI
WormBase CE36465 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA79545 Hypothetical protein B0464.8 [Caenorhabditis elegans] ref|NP_499086.2| B0464.8 [Caenorhabditis elegans] 0.0
blast CAE65208 Hypothetical protein CBG10083 [Caenorhabditis briggsae] 3.0E-99
blast CAE65208 Hypothetical protein CBG10083 [Caenorhabditis briggsae] 7.0E-97
Sequence
Target Sequence 
>C. ELEGANS:178222_AT
aatacagtcgattcgcacttgctcgacgtgtcagtttccagaatcaatcagcagttcctg
tggtttatagtgacacattttcaaagtcaaatgcaagaaaaagaaaacgatcgcctccaa
caaacaatcgtcaacttctgctccaatggcttggaaatccaccagttcacgacgaatccg
ctcaagtttttctcgcatttctcgccaaagatattatctcggagatcgttggaaatgccc
ttctggagcgacgaaaaatgaatcccgcagaaggaggtccactgagctactcttattacg
aggaacctttgcgcagaaaca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATACAGTCGATTCGCACTTGCTCG 661 171 575 Antisense
CTTGCTCGACGTGTCAGTTTCCAGA 643 217 592 Antisense
GAATCAATCAGCAGTTCCTGTGGTT 559 325 615 Antisense
CGATCGCCTCCAACAAACAATCGTC 677 245 682 Antisense
GAAATCCACCAGTTCACGACGAATC 479 361 728 Antisense
CGACGAATCCGCTCAAGTTTTTCTC 373 225 744 Antisense
TTCTCGCATTTCTCGCCAAAGATAT 453 691 764 Antisense
GAGATCGTTGGAAATGCCCTTCTGG 229 395 796 Antisense
GAATCCCGCAGAAGGAGGTCCACTG 93 329 834 Antisense
GGTCCACTGAGCTACTCTTATTACG 710 501 850 Antisense
TACGAGGAACCTTTGCGCAGAAACA 323 647 871 Antisense
Sequence Source Affymetrix Proprietary Database