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[178219_at]

Description F32H5.2 /REP_DB=WormBase Gene ID /WP=CE17750 /TR=O62209 /GB=CAB04251.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 25      4grp_total > 14      6grp_cgc > 32      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 90
1 2 3 4 range
A 135.6
(3)
P
59
(-3)
A
80.4
(-1.3)
A
110.7
(1)
A
77
B 98.5
(3)
P
69.8
(-0.7)
P
52.3
(-3)
A
94
(2.4)
P
46
C 88.3
(-0.6)
P
53.1
(-3)
A
65.8
(-2.1)
A
142.6
(3)
P
90
avr 107.5
(2.1)
60.6
(-3)
66.2
(-2.4)
115.8
(3)
55

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 106
1 2 3 4 5 6 range
A 127.6
(3)
P
70.7
(-0.4)
M
85.9
(0.5)
M
50.9
(-1.5)
P
26.4
(-3)
A
33.6
(-2.6)
A
101
B 110.7
(3)
P
108.8
(2.8)
P
101.3
(2.2)
P
47.7
(-2.4)
A
61.6
(-1.2)
P
41.2
(-3)
A
70
C 95
(1.3)
A
123.1
(3)
P
60.7
(-0.7)
A
21.7
(-3)
A
67.9
(-0.3)
A
40.9
(-1.9)
A
101
avr 111.1
(3)
100.9
(2.2)
82.6
(0.6)
40.1
(-2.9)
52
(-1.9)
38.6
(-3)
73
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 37
1 2 3 4 range
A 49.4
(3)
P
12.8
(-3)
A
21
(-1.7)
A
16.2
(-2.4)
A
37
B 31.4
(3)
P
17.2
(-1.4)
P
12.1
(-3)
A
20
(-0.5)
P
19
C 29.2
(3)
P
15.3
(-3)
A
22.9
(0.3)
A
24.1
(0.8)
P
14
avr 36.7
(3)
15.1
(-3)
18.7
(-2)
20.1
(-1.6)
22

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 34
1 2 3 4 5 6 range
A 23.5
(2.4)
P
25.2
(3)
M
18.7
(0.7)
M
12.9
(-1.3)
P
8
(-3)
A
8.2
(-2.9)
A
17
B 38.5
(3)
P
36.2
(2.5)
P
24.6
(-0.2)
P
12.6
(-3)
A
25
(-0.1)
P
15.4
(-2.4)
A
26
C 35.2
(2.6)
A
37.5
(3)
P
15.4
(-1)
A
4.4
(-3)
A
22
(0.2)
A
13.7
(-1.3)
A
33
avr 32.4
(2.8)
33
(3)
19.6
(-0.5)
10
(-3)
18.3
(-0.8)
12.4
(-2.4)
23
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178219_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13117
Sequence Type Exemplar sequence
Representative Public ID F32H5.2 NCBI
Target Description F32H5.2 /REP_DB=WormBase Gene ID /WP=CE17750 /TR=O62209 /GB=CAB04251.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_182337, NM_182338
Transcript Assignments
Representative
Transcript
Description Matching Probes Related Probesets by Grade
GENEFINDER00000043411 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:13351373:13358596:1 None
NM_182338 NCBI Caenorhabditis elegans F32H5.8 (F32H5.8) mRNA, complete cds. None
NM_182337 NCBI Caenorhabditis elegans TWiK family of potassium channels family member (twk-43) (twk-43) mRNA, complete cds. None
F32H5.7 ENSEMBL cdna:known chromosome:CEL140:V:13354203:13357865:1 gene:F32H5.7 None
SNAP00000043407 ENSEMBL cdna:SNAP chromosome:CEL140:V:13354056:13358596:1 None
F32H5.8 ENSEMBL cdna:known chromosome:CEL140:V:13356865:13358693:1 gene:F32H5.8 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:13354178-13358596(+) 99.89 99.89
Public Domain and Genome References
Gene Title

Gene Symbol twk-43
Ensembl F32H5.7
F32H5.8
Entrez Gene 3565024 Entrez gene
3565836 Entrez gene
SwissProt Q8I4K1 EMBL-EBI
Q8I4K0 EMBL-EBI
WormBase CE31925 Wormbase
CE31926 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAE70750 Hypothetical protein CBG17497 [Caenorhabditis briggsae] 0.0
blast CAD54142 Hypothetical protein F32H5.7 [Caenorhabditis elegans] ref|NP_872137.1| TWiK family of potassium channels family member (twk-43) [Caenorhabditis elegans] 1.0E-115
blast CAD54142 Hypothetical protein F32H5.7 [Caenorhabditis elegans] ref|NP_872137.1| TWiK family of potassium channels family member (twk-43) [Caenorhabditis elegans] 1.0E-110
blast CAE70750 Hypothetical protein CBG17497 [Caenorhabditis briggsae] 1.0E-105
blast CAD54128 Hypothetical protein F32H5.2 [Caenorhabditis elegans] emb|CAB04251.2| Hypothetical protein F32H5.2 [Caenorhabditis elegans] ref|NP_506309.2| F32H5.2 [Caenorhabditis elegans] 1.0E-77
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_872137.1 3 85-104,109-131,138-160
F32H5.7 3 85-104,109-131,138-160
SNAP00000043407 6 10-32,139-161,165-187,262-281,286-308,315-337
GENEFINDER00000043411 7 21-39,85-107,217-239,243-265,340-359,364-386,393-415
Sequence
Target Sequence
>C. ELEGANS:178219_AT
acctcgctaaatttgtcgatgacgcaatcaaagtagaagctggagagttgacaacactga
gggttagctttgttgcaataaatttatataggaacgagccagggacgacggcaacttatg
ccttttttaacgaccgtgcctggatgaattctaattattggcgtgcaatagaaagtggtt
caatgcactacgtggatgatgatgatcttgtgacattacagtcaaatctatcatgtggct
cacagcaaatgctcatcagagttccatactcaagatcgacagattcccgaatgattactg
taaatcatgagcctttgacagagacttcggatgaaaacttcaaacttcatagtgtaaatg
aagaatacgaaactgaacagtttccagatattgaagctgaaattatagataaaccggaag
ttagccaaaatgaaggaatccgaaaaagttcccaatgcattgaacttcagaaacttctga
aagctttaaacgatttgaacaacgaagtagtgtcacaagatggccgatggagtgaagaag
ctagacggcgatacctggaat


BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position
Strandedness
ACCTCGCTAAATTTGTCGATGACGC 485 87 1169 Antisense
GGAACGAGCCAGGGACGACGGCAAC 124 529 1259 Antisense
GGGACGACGGCAACTTATGCCTTTT 543 493 1270 Antisense
TATGCCTTTTTTAACGACCGTGCCT 675 611 1285 Antisense
AACGACCGTGCCTGGATGAATTCTA 346 145 1297 Antisense
GTCAAATCTATCATGTGGCTCACAG 182 465 1389 Antisense
GTGGCTCACAGCAAATGCTCATCAG 258 491 1403 Antisense
AGTTCCATACTCAAGATCGACAGAT 109 65 1428 Antisense
GAGCCTTTGACAGAGACTTCGGATG 624 385 1477 Antisense
GTAGTGTCACAAGATGGCCGATGGA 97 457 1675 Antisense
GAAGCTAGACGGCGATACCTGGAAT 664 341 1705 Antisense
Sequence Source Affymetrix Proprietary Database