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[178189_at]

Description F28C6.5 /REP_DB=WormBase Gene ID /WP=CE03276 /TR=Q19865 /GB=CAA92671.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 29      6grp_high1_10_10 > 81      4grp_highest_8_8 > 03      4grp_high_8_8 > 47      6grp_highest_nonorm > 53      6grp_total > 92      4grp_total > 41      6grp_highest_sub > 04_05      6grp_high1_sub > 04      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 710
1 2 3 4 range
A 292.2
(-3)
P 		871.4
(1.9)
P 		995.6
(3)
P 		530.4
(-1)
P 		703
B 302.4
(-3)
P 		788.2
(2.6)
P 		820.7
(3)
P 		524
(-0.4)
P 		518
C 285.7
(-3)
P 		848.7
(3)
P 		847.6
(3)
P 		467.7
(-1.1)
P 		563
avr 293.4
(-3) 		836.1
(2.5) 		888
(3) 		507.4
(-0.8) 		595

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1503
1 2 3 4 5 6 range
A 363.2
(-2.8)
P 		670.6
(-1.5)
P 		526.4
(-2.1)
P 		1730
(3)
P 		1310
(1.2)
P 		307.8
(-3)
P 		1422
B 227.4
(-3)
P 		607.6
(-1.4)
P 		439
(-2.1)
P 		1256.2
(1.4)
P 		1617.5
(3)
P 		352.5
(-2.5)
P 		1390
C 255.6
(-3)
P 		690.6
(-0.9)
P 		563.4
(-1.5)
P 		1473.7
(3)
P 		1321.7
(2.3)
P 		348
(-2.5)
P 		1218
avr 282.1
(-3) 		656.3
(-1.1) 		509.6
(-1.9) 		1486.6
(3) 		1416.4
(2.7) 		336.1
(-2.7) 		1205
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 218
1 2 3 4 range
A 106.4
(-2.1)
P 		189.2
(0.7)
P 		260.3
(3)
P 		77.6
(-3)
P 		183
B 96.4
(-3)
P 		194.7
(3)
P 		190.2
(2.7)
P 		111.7
(-2.1)
P 		98
C 94.6
(-2.6)
P 		244
(1.6)
P 		295.4
(3)
P 		78.9
(-3)
P 		217
avr 99.1
(-2.6) 		209.3
(1.5) 		248.6
(3) 		89.4
(-3) 		159

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 590
1 2 3 4 5 6 range
A 67
(-3)
P 		238.5
(-0.2)
P 		114.6
(-2.2)
P 		438.8
(3)
P 		395.3
(2.3)
P 		74.9
(-2.9)
P 		372
B 79.1
(-3)
P 		202.2
(-1.7)
P 		106.6
(-2.7)
P 		332.7
(-0.4)
P 		657.2
(3)
P 		131.4
(-2.5)
P 		578
C 94.7
(-3)
P 		210.7
(-0.9)
P 		143.4
(-2.1)
P 		296.4
(0.6)
P 		427.7
(3)
P 		116.5
(-2.6)
P 		333
avr 80.3
(-3) 		217.1
(-1) 		121.5
(-2.4) 		356
(1) 		493.4
(3) 		107.6
(-2.6) 		413
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178189_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3830
Sequence Type Exemplar sequence
Representative Public ID F28C6.5 NCBI
Target Description F28C6.5 /REP_DB=WormBase Gene ID /WP=CE03276 /TR=Q19865 /GB=CAA92671.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063420(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063420 NCBI Caenorhabditis elegans F28C6.5 (F28C6.5) mRNA, complete cds. 11/11 None
SNAP00000038092 ENSEMBL cdna:SNAP chromosome:CEL140:II:8599477:8600362:1 11/11 None
GENEFINDER00000038099 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:8599477:8600362:1 11/11 None
F28C6.5 ENSEMBL cdna:known chromosome:CEL140:II:8599601:8600362:1 gene:F28C6.5 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:8599597-8600359(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F28C6.5
Entrez Gene 185048 Entrez gene
SwissProt Q19865 EMBL-EBI
WormBase CE03276 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA92671 Hypothetical protein F28C6.5 [Caenorhabditis elegans] ref|NP_495821.1| F28C6.5 [Caenorhabditis elegans] 1.0E-107
blast CAA92671 Hypothetical protein F28C6.5 [Caenorhabditis elegans] ref|NP_495821.1| F28C6.5 [Caenorhabditis elegans] 3.0E-85
blast CAE57571 Hypothetical protein CBG00549 [Caenorhabditis briggsae] 1.0E-74
blast CAE57571 Hypothetical protein CBG00549 [Caenorhabditis briggsae] 6.0E-70
Sequence
Target Sequence 
>C. ELEGANS:178189_AT
tgtctccattctatcctgcagatggaaacccatcaaatccgttcccacctatatataaag
ctcgtcctctacctcttcatgtcccagagccacaagacgtcgtagccgttccattagatg
aagaagctcttcacggagaaccactattcccgccagagctcattcttccgagtgaactgg
ctgcagaagaagcggcaaagaaaaaggctttggagacaaagaaacctgagaatccatttg
ctgacgttgttgaaaccaatgagcagttggatactgtacattggccaacggcagtgagta
catcagagatagaaggagaggactcacagggaaagaatatggttcaagaagatgaaccac
aacgctcagtaataatcgatgaagcgagagagaaacccatgcagccatttgaagcagcca
agatatcaattggtgcgaacgaccggccga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGTCTCCATTCTATCCTGCAGATGG 451 557 179 Antisense
TGGAAACCCATCAAATCCGTTCCCA 277 547 201 Antisense
TATATATAAAGCTCGTCCTCTACCT 549 659 228 Antisense
CCCAGAGCCACAAGACGTCGTAGCC 307 259 261 Antisense
CGTCGTAGCCGTTCCATTAGATGAA 476 243 276 Antisense
GAAGCTCTTCACGGAGAACCACTAT 221 343 301 Antisense
CAGAGCTCATTCTTCCGAGTGAACT 622 205 332 Antisense
GAATCCATTTGCTGACGTTGTTGAA 118 329 408 Antisense
TGGCCAACGGCAGTGAGTACATCAG 53 545 460 Antisense
GAGAGAAACCCATGCAGCCATTTGA 383 393 566 Antisense
TCAATTGGTGCGAACGACCGGCCGA 591 633 604 Antisense
Sequence Source Affymetrix Proprietary Database