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[178186_at]

Description F25H5.8 /REP_DB=WormBase Gene ID /WP=CE24917 /TR=Q9NAQ5 /GB=CAB82207.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 99      4grp_total > 02      6grp_total_sub > Increase      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 196
1 2 3 4 range
A 6.8
(-3)
A 		36.7
(-2)
A 		71.4
(-0.8)
A 		179.4
(3)
A 		173
B 6.1
(-3)
A 		18.2
(-2.5)
A 		23.8
(-2.2)
A 		140.1
(3)
A 		134
C 46.8
(-1.7)
A 		22.4
(-2.5)
A 		5.6
(-3)
A 		201.9
(3)
A 		196
avr 19.9
(-3) 		25.8
(-2.8) 		33.6
(-2.5) 		173.8
(3) 		154

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2390
1 2 3 4 5 6 range
A 886.9
(-1)
A 		84.2
(-3)
A 		231.7
(-2.6)
A 		129.9
(-2.9)
A 		2439.3
(3)
P 		1217.6
(-0.1)
P 		2355
B 704.4
(-1.5)
P 		150.5
(-2.9)
A 		101.5
(-3)
A 		194.2
(-2.8)
A 		2474.2
(3)
P 		1729.4
(1.1)
P 		2373
C 481.6
(-1.6)
P 		159.3
(-2.7)
A 		89.8
(-3)
A 		264.9
(-2.4)
M 		1746.9
(3)
P 		771.7
(-0.5)
P 		1657
avr 691
(-1.4) 		131.3
(-3) 		141
(-3) 		196.3
(-2.8) 		2220.1
(3) 		1239.6
(0.2) 		2089
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 32
1 2 3 4 range
A 2.5
(-3)
A 		8
(-1.6)
A 		18.7
(1.1)
A 		26.3
(3)
A 		24
B 2
(-3)
A 		4.5
(-2.5)
A 		5.5
(-2.2)
A 		29.9
(3)
A 		28
C 15.5
(-0.5)
A 		6.5
(-2.1)
A 		1.9
(-3)
A 		34
(3)
A 		32
avr 6.7
(-2.9) 		6.3
(-3) 		8.7
(-2.4) 		30.1
(3) 		24

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 982
1 2 3 4 5 6 range
A 163.5
(-1.9)
A 		29.9
(-3)
A 		50.5
(-2.8)
A 		32.9
(-3)
A 		736.1
(3)
P 		296.4
(-0.7)
P 		706
B 245.1
(-1.7)
P 		50.1
(-2.8)
A 		24.6
(-3)
A 		51.4
(-2.8)
A 		1005.3
(3)
P 		644.9
(0.8)
P 		981
C 178.5
(-1.3)
P 		48.6
(-2.7)
A 		22.9
(-3)
A 		53.3
(-2.7)
M 		565.3
(3)
P 		258.3
(-0.4)
P 		542
avr 195.7
(-1.7) 		42.9
(-2.9) 		32.7
(-3) 		45.9
(-2.9) 		768.9
(3) 		399.9
(-0) 		736
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178186_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.812
Sequence Type Exemplar sequence
Representative Public ID F25H5.8 NCBI
Target Description F25H5.8 /REP_DB=WormBase Gene ID /WP=CE24917 /TR=Q9NAQ5 /GB=CAB82207.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060059(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060059 NCBI Caenorhabditis elegans F25H5.8 (F25H5.8) mRNA, complete cds. 11/11 None
SNAP00000024322 ENSEMBL cdna:SNAP chromosome:CEL140:I:9185871:9186821:-1 11/11 None
GENEFINDER00000024332 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:9185871:9186821:-1 11/11 None
F25H5.8 ENSEMBL cdna:known chromosome:CEL140:I:9185871:9186821:-1 gene:F25H5.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:9185801-9186752(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F25H5.8
Entrez Gene 184939 Entrez gene
WormBase CE24917 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB82207 Hypothetical protein F25H5.8 [Caenorhabditis elegans] ref|NP_492460.1| F25H5.8 [Caenorhabditis elegans] 8.0E-30
blast CAE61306 Hypothetical protein CBG05141 [Caenorhabditis briggsae] 2.0E-21
blast NP_001021675 W10C8.6 [Caenorhabditis elegans] gb|AAY55881.1| Hypothetical protein W10C8.6 [Caenorhabditis elegans] 4.0E-13
Protein Domains
Database ID Description E-Value
Pfam IPR000612 EMBL-EBI Protein of unknown function UPF0057 1.6E-18
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_492460.1 2 9-26,36-58
F25H5.8 2 9-26,36-58
SNAP00000024322 2 9-26,36-58
GENEFINDER00000024332 2 9-26,36-58
Sequence
Target Sequence 
>C. ELEGANS:178186_AT
ggcggaaactcctgaagataagattgttatggttctactcattcttcttttcccgccatt
agcggtatggtacaaggaaaagacgtgtggagttggtgtctgtatcaacgtggttctcta
cattctgctcattttcccagcatatattcatgcagtt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGCGGAAACTCCTGAAGATAAGATT 275 539 15 Antisense
GATTGTTATGGTTCTACTCATTCTT 227 435 36 Antisense
TATGGTTCTACTCATTCTTCTTTTC 187 655 42 Antisense
TCTTCTTTTCCCGCCATTAGCGGTA 675 613 57 Antisense
TCCCGCCATTAGCGGTATGGTACAA 462 595 65 Antisense
GCGGTATGGTACAAGGAAAAGACGT 584 291 76 Antisense
ACGTGTGGAGTTGGTGTCTGTATCA 466 95 97 Antisense
TGGTGTCTGTATCAACGTGGTTCTC 269 551 108 Antisense
TATCAACGTGGTTCTCTACATTCTG 245 659 117 Antisense
CTGCTCATTTTCCCAGCATATATTC 616 237 139 Antisense
TTTCCCAGCATATATTCATGCAGTT 121 671 147 Antisense
Sequence Source Affymetrix Proprietary Database