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[178182_at]

Description F44G4.5 /REP_DB=WormBase Gene ID /WP=CE02232 /TR=Q20415 /GB=CAA90121.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 99      4grp_total > 43      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 51
1 2 3 4 range
A 13.8
(-3)
A 		41
(3)
A 		18.3
(-2)
A 		23.3
(-0.9)
A 		27
B 41.2
(3)
A 		31
(-3)
A 		38.7
(1.5)
A 		38.4
(1.4)
A 		10
C 26.1
(-0.6)
A 		56.7
(3)
A 		5.5
(-3)
A 		21.6
(-1.1)
A 		51
avr 27
(-1.3) 		42.9
(3) 		20.8
(-3) 		27.8
(-1.1) 		22

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 126
1 2 3 4 5 6 range
A 11.6
(-3)
A 		39
(-1.1)
A 		16.2
(-2.7)
A 		12
(-3)
A 		96.5
(3)
P 		87.2
(2.3)
P 		85
B 29.2
(-1.7)
A 		7.1
(-3)
A 		6.3
(-3)
A 		8.5
(-2.9)
A 		110.5
(3)
P 		79.4
(1.2)
P 		104
C 46.3
(-1)
A 		5.4
(-2.9)
A 		4.2
(-3)
A 		13.6
(-2.6)
A 		118.5
(2.4)
P 		130.6
(3)
P 		126
avr 29
(-1.8) 		17.2
(-2.5) 		8.9
(-3) 		11.4
(-2.8) 		108.5
(3) 		99.1
(2.4) 		100
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 14
1 2 3 4 range
A 5
(-1.3)
A 		8.9
(3)
A 		4.8
(-1.5)
A 		3.4
(-3)
A 		6
B 13.1
(3)
A 		7.7
(-3)
A 		9
(-1.6)
A 		8.2
(-2.4)
A 		5
C 8.6
(-0.2)
A 		16.3
(3)
A 		1.9
(-3)
A 		3.6
(-2.3)
A 		14
avr 8.9
(0.9) 		11
(3) 		5.2
(-2.9) 		5.1
(-3) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 44
1 2 3 4 5 6 range
A 2.1
(-3)
A 		13.9
(-0.4)
A 		3.5
(-2.7)
A 		3.1
(-2.8)
A 		29.1
(3)
P 		21.2
(1.2)
P 		27
B 10.2
(-1.8)
A 		2.4
(-2.9)
A 		1.5
(-3)
A 		2.3
(-2.9)
A 		44.9
(3)
P 		29.6
(0.9)
P 		43
C 17.2
(-0.7)
A 		1.7
(-2.9)
A 		1.1
(-3)
A 		2.7
(-2.8)
A 		38.3
(2.2)
P 		43.7
(3)
P 		43
avr 9.8
(-1.7) 		6
(-2.3) 		2
(-3) 		2.7
(-2.9) 		37.4
(3) 		31.5
(2) 		35
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178182_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3055
Sequence Type Exemplar sequence
Representative Public ID F44G4.5 NCBI
Target Description F44G4.5 /REP_DB=WormBase Gene ID /WP=CE02232 /TR=Q20415 /GB=CAA90121.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063521(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063521 NCBI Caenorhabditis elegans F44G4.5 (F44G4.5) mRNA, complete cds. 11/11 None
SNAP00000000992 ENSEMBL cdna:SNAP chromosome:CEL140:II:8999690:9000228:-1 10/11 None
GENEFINDER00000001002 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:8999690:9000282:-1 11/11 None
F44G4.5 ENSEMBL cdna:known chromosome:CEL140:II:8999690:9000282:-1 gene:F44G4.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:8999686-9000279(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F44G4.5
Entrez Gene 185761 Entrez gene
SwissProt Q20415 EMBL-EBI
WormBase CE02232 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA90121 Hypothetical protein F44G4.5 [Caenorhabditis elegans] ref|NP_495922.1| F44G4.5 [Caenorhabditis elegans] 3.0E-99
blast CAA90121 Hypothetical protein F44G4.5 [Caenorhabditis elegans] ref|NP_495922.1| F44G4.5 [Caenorhabditis elegans] 8.0E-89
blast CAE59784 Hypothetical protein CBG03236 [Caenorhabditis briggsae] 8.0E-47
blast CAE59784 Hypothetical protein CBG03236 [Caenorhabditis briggsae] 2.0E-44
blast CAB03814 Hypothetical protein C01G12.3 [Caenorhabditis elegans] ref|NP_497030.1| C01G12.3 [Caenorhabditis elegans] 1.0E-37
Sequence
Target Sequence 
>C. ELEGANS:178182_AT
gatcatgcagccaactttcaacttcaatggataatattcaacgaaatttgatgcttctgg
aaattgagaagaatgaaaagtcgagctttgatgacgtcatcacgggtgccgaatcggata
atggttgggaggattgtttgagagaaactgaaagtgttcaaagtcaatggatcactgaac
aatccaccattgcttatccagaaactacacgtgtcaattgtgatggtccacaaagttatt
atgaattggctgatttgaattataagccagcagagaatactgttcgtttggctcgtgtcc
catcacagtaccaacttgatgctttgtccgagattttcgttttcgatgttcctcaacata
ttgccgttaaaaaagttgaagcttcatttctgccgaaatatgtgaacacgagttccgagg
ttgcccagaattctgtgaacacgacgccatactttgtgaattcggag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATCATGCAGCCAACTTTCAACTTC 376 421 41 Antisense
ATGACGTCATCACGGGTGCCGAATC 360 45 131 Antisense
GAACAATCCACCATTGCTTATCCAG 177 353 217 Antisense
GCTTATCCAGAAACTACACGTGTCA 26 305 232 Antisense
GAATACTGTTCGTTTGGCTCGTGTC 607 325 315 Antisense
GTACCAACTTGATGCTTTGTCCGAG 60 459 348 Antisense
TTTTCGTTTTCGATGTTCCTCAACA 680 675 374 Antisense
AGTTGAAGCTTCATTTCTGCCGAAA 152 63 414 Antisense
TTCCGAGGTTGCCCAGAATTCTGTG 297 699 453 Antisense
GAATTCTGTGAACACGACGCCATAC 157 325 468 Antisense
GACGCCATACTTTGTGAATTCGGAG 259 381 483 Antisense
Sequence Source Affymetrix Proprietary Database