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[178177_at]

Description F25H5.2 /REP_DB=WormBase Gene ID /WP=CE15897 /TR=O17834 /GB=CAB02982.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 96      4grp_total > 0      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 119
1 2 3 4 range
A 67.8
(0.1)
A 		10.6
(-3)
A 		71.6
(0.3)
A 		121
(3)
A 		110
B 46.8
(-3)
A 		50.9
(-2.7)
A 		74.6
(-1)
A 		129.6
(3)
A 		83
C 57.8
(-1.6)
A 		41.8
(-3)
A 		45
(-2.7)
A 		110.5
(3)
A 		69
avr 57.5
(-1.4) 		34.4
(-3) 		63.7
(-1) 		120.4
(3) 		86

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 138
1 2 3 4 5 6 range
A 39
(-1.7)
A 		8.8
(-3)
A 		45.6
(-1.4)
A 		47.2
(-1.3)
A 		129
(2.2)
P 		146.3
(3)
P 		138
B 82.9
(1.2)
A 		72.5
(0.1)
A 		44.2
(-3)
A 		98.9
(3)
A 		80.4
(1)
P 		94.4
(2.5)
P 		55
C 46.9
(-3)
A 		59.9
(-2)
A 		51
(-2.7)
A 		95.2
(0.9)
A 		71.5
(-1)
P 		121.9
(3)
P 		75
avr 56.3
(-2.2) 		47.1
(-3) 		46.9
(-3) 		80.4
(-0.3) 		93.6
(0.8) 		120.9
(3) 		74
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 25
1 2 3 4 range
A 24.7
(3)
A 		2.3
(-3)
A 		18.7
(1.4)
A 		17.7
(1.1)
A 		22
B 14.9
(-2.1)
A 		12.6
(-3)
A 		17.3
(-1.1)
A 		27.6
(3)
A 		15
C 19.1
(3)
A 		12
(-3)
A 		15.7
(0.1)
A 		18.6
(2.6)
A 		7
avr 19.6
(2.2) 		9
(-3) 		17.2
(1) 		21.3
(3) 		12

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 38
1 2 3 4 5 6 range
A 7.2
(-2.3)
A 		3.1
(-3)
A 		9.9
(-1.9)
A 		12
(-1.5)
A 		38.9
(3)
P 		35.6
(2.4)
P 		36
B 28.8
(1.4)
A 		24.1
(0.3)
A 		10.7
(-3)
A 		26.2
(0.8)
A 		32.6
(2.4)
P 		35.2
(3)
P 		25
C 17.4
(-2.1)
A 		18.3
(-1.9)
A 		13
(-3)
A 		19.2
(-1.7)
A 		23.2
(-0.8)
P 		40.8
(3)
P 		28
avr 17.8
(-1.5) 		15.2
(-2.1) 		11.2
(-3) 		19.1
(-1.2) 		31.6
(1.7) 		37.2
(3) 		26
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178177_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.921
Sequence Type Exemplar sequence
Representative Public ID F25H5.2 NCBI
Target Description F25H5.2 /REP_DB=WormBase Gene ID /WP=CE15897 /TR=O17834 /GB=CAB02982.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060060(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060060 NCBI Caenorhabditis elegans F25H5.2 (F25H5.2) mRNA, complete cds. 11/11 None
SNAP00000024323 ENSEMBL cdna:SNAP chromosome:CEL140:I:9188097:9189127:-1 11/11 None
GENEFINDER00000024329 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:9188097:9189127:-1 11/11 None
F25H5.2 ENSEMBL cdna:known chromosome:CEL140:I:9188097:9189127:-1 gene:F25H5.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:9188027-9189058(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F25H5.2
Entrez Gene 184938 Entrez gene
WormBase CE15897 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB02982 Hypothetical protein F25H5.2 [Caenorhabditis elegans] ref|NP_492461.1| F25H5.2 [Caenorhabditis elegans] 1.0E-135
blast CAE65687 Hypothetical protein CBG10762 [Caenorhabditis briggsae] 6.0E-44
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_492461.1 1 158-180
F25H5.2 1 158-180
SNAP00000024323 1 158-180
GENEFINDER00000024329 1 158-180
Sequence
Target Sequence 
>C. ELEGANS:178177_AT
ccataaacaccagccaacatttgccccttgcaaatatggagtcgcaatgtcatttgatca
accacattttgtggctgatccaacggatgactttccagaagaaagagtctgttgtacagg
atgtcaaacacttcatatcaaacttcccggcacagacactaatgccactttatgttgtaa
tcctcaaacaccgtataggcattctcttggccgaattgtttctggatcagacaagaacag
tcctggtcacgttagcggcaattttggatatgttcatccgttgcacttagccttcgataa
aattgctccgaaagaattgcttgtgaaagatgaaaaaccaagagaaacaagaattggaca
aggaatgtggccatttatgttcatcggagcagcagctgctcaatctgtcattctcatttt
ggctttactcgtttgcaagttcactgataagaagattattctggaaactgtaccggattg
tgattatgaagtgattaaagaagctcctgtcatcagtcatcagtcga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCATAAACACCAGCCAACATTTGCC 74 271 120 Antisense
TTGCCCCTTGCAAATATGGAGTCGC 211 703 140 Antisense
TCATATCAAACTTCCCGGCACAGAC 175 619 252 Antisense
AATGCCACTTTATGTTGTAATCCTC 516 147 280 Antisense
AACACCGTATAGGCATTCTCTTGGC 245 135 306 Antisense
TCCTGGTCACGTTAGCGGCAATTTT 301 593 360 Antisense
GATATGTTCATCCGTTGCACTTAGC 671 425 386 Antisense
TTATGTTCATCGGAGCAGCAGCTGC 109 679 494 Antisense
GTCATTCTCATTTTGGCTTTACTCG 423 467 526 Antisense
GGCTTTACTCGTTTGCAAGTTCACT 459 537 540 Antisense
GCTCCTGTCATCAGTCATCAGTCGA 349 299 622 Antisense
Sequence Source Affymetrix Proprietary Database