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[178163_at]

Description B0250.2 /REP_DB=WormBase Gene ID /WP=CE18479 /TR=Q9XVF6 /GB=CAB03793.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 01      6grp_total > 33      4grp_total > 29      6grp_high1_sub > De      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 162
1 2 3 4 range
A 227.6
(3)
P 		172.8
(1)
A 		65.5
(-3)
A 		206.6
(2.2)
P 		162
B 155.5
(-0.2)
P 		168.5
(1.6)
M 		136.4
(-3)
A 		178
(3)
A 		42
C 171.3
(1)
P 		160.2
(-0.4)
A 		138.7
(-3)
A 		187.4
(3)
A 		49
avr 184.8
(2.5) 		167.2
(1.2) 		113.5
(-3) 		190.7
(3) 		77

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 315
1 2 3 4 5 6 range
A 402.6
(3)
P 		169.2
(-1.5)
P 		251
(0.1)
P 		197.7
(-0.9)
P 		121
(-2.4)
A 		88.1
(-3)
A 		315
B 293.6
(3)
P 		243.8
(1.2)
P 		202.2
(-0.2)
P 		124.7
(-3)
P 		171.5
(-1.3)
P 		129.1
(-2.8)
A 		169
C 166.7
(-1)
P 		149.1
(-2.2)
P 		220.5
(2.6)
M 		225.9
(3)
P 		144.2
(-2.6)
A 		138
(-3)
P 		88
avr 287.6
(3) 		187.4
(-0.6) 		224.6
(0.8) 		182.8
(-0.7) 		145.6
(-2) 		118.4
(-3) 		169
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 66
1 2 3 4 range
A 82.9
(3)
P 		37.5
(-1.1)
A 		17.1
(-3)
A 		30.2
(-1.8)
P 		66
B 49.6
(3)
P 		41.6
(0.3)
M 		31.6
(-3)
A 		37.9
(-0.9)
A 		18
C 56.7
(3)
P 		46
(0.4)
A 		48.4
(1)
A 		31.6
(-3)
A 		25
avr 63.1
(3) 		41.7
(-1.2) 		32.4
(-3) 		33.2
(-2.8) 		31

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 81
1 2 3 4 5 6 range
A 74.2
(3)
P 		60.2
(1.4)
P 		54.7
(0.8)
P 		50.1
(0.3)
P 		36.5
(-1.3)
A 		21.4
(-3)
A 		53
B 102.2
(3)
P 		81.1
(1.2)
P 		49.1
(-1.6)
P 		33
(-3)
P 		69.7
(0.2)
P 		48.1
(-1.7)
A 		69
C 61.8
(3)
P 		45.5
(-3)
P 		56.1
(0.9)
M 		45.4
(-3)
P 		46.7
(-2.5)
A 		46.2
(-2.7)
P 		16
avr 79.4
(3) 		62.3
(0.5) 		53.3
(-0.8) 		42.8
(-2.4) 		51
(-1.2) 		38.6
(-3) 		41
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178163_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13632
Sequence Type Exemplar sequence
Representative Public ID B0250.2 NCBI
Target Description B0250.2 /REP_DB=WormBase Gene ID /WP=CE18479 /TR=Q9XVF6 /GB=CAB03793.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075538(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075538 NCBI Caenorhabditis elegans B0250.2 (B0250.2) mRNA, complete cds. 11/11 None
SNAP00000043589 ENSEMBL cdna:SNAP chromosome:CEL140:V:20474936:20476970:-1 11/11 None
GENEFINDER00000043600 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:20474857:20476970:-1 9/11 None
B0250.2 ENSEMBL cdna:known chromosome:CEL140:V:20474805:20476970:-1 gene:B0250.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:20474935-20476970(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl B0250.2
Entrez Gene 180342 Entrez gene
SwissProt Q9XVF6 EMBL-EBI
WormBase CE18479 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB03793 Hypothetical protein B0250.2 [Caenorhabditis elegans] ref|NP_507939.1| B0250.2 [Caenorhabditis elegans] 2.0E-80
blast CAE61653 Hypothetical protein CBG05587 [Caenorhabditis briggsae] 7.0E-69
blast CAB03793 Hypothetical protein B0250.2 [Caenorhabditis elegans] ref|NP_507939.1| B0250.2 [Caenorhabditis elegans] 1.0E-35
blast CAE61653 Hypothetical protein CBG05587 [Caenorhabditis briggsae] 2.0E-33
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_507939.1 1 15-37
B0250.2 1 15-37
SNAP00000043589 1 15-37
GENEFINDER00000043600 1 15-37
Sequence
Target Sequence 
>C. ELEGANS:178163_AT
aattcacaattccggcgagcgaggtgatgacgtcaattgtgctcccgacgataattttct
gtgttgggctcgttgcagccatgttgcttggaattaataaatgcaagcagtgggagctgg
acaaggtcgtcgagatgagaagatcacgtcgggtgctccagcgagttacgaataggctta
gagagaagaattttaagacgatacaacgagctcggacaatgcgaaaaagggccgccagtg
caaaagcaagaaatgaattctcaccgccggcttatcaatcaaaaaacaattcgacagagt
ttccgtgtggcacatctcctccgccatcgtatgaagacgacgttctcgaggagtactaca
aagaatgtcacccgagcagacgatctatcccggtacctagctctcctcgacgatatattc
gacaaaggtcgaaattcagttctgcagcaagaagagtcagccgttcaagtgtcagcac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATTCACAATTCCGGCGAGCGAGGT 708 167 29 Antisense
GTGATGACGTCAATTGTGCTCCCGA 350 483 52 Antisense
TGCTCCCGACGATAATTTTCTGTGT 672 577 68 Antisense
CTCGTTGCAGCCATGTTGCTTGGAA 407 233 97 Antisense
TGAGAAGATCACGTCGGGTGCTCCA 280 569 164 Antisense
TCGGGTGCTCCAGCGAGTTACGAAT 110 607 177 Antisense
AAAGGGCCGCCAGTGCAAAAGCAAG 515 121 254 Antisense
GACAGAGTTTCCGTGTGGCACATCT 232 367 321 Antisense
TGTCACCCGAGCAGACGATCTATCC 444 559 394 Antisense
CCTAGCTCTCCTCGACGATATATTC 190 269 424 Antisense
GAGTCAGCCGTTCAAGTGTCAGCAC 123 401 482 Antisense
Sequence Source Affymetrix Proprietary Database