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[178157_at]

Description F53F8.5 /REP_DB=WormBase Gene ID /WP=CE17859 /TR=O62258 /GB=CAB04458.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 17      4grp_total > 11      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 42
1 2 3 4 range
A 9.2
(-2.4)
A 		5.3
(-3)
A 		8.9
(-2.5)
A 		46.4
(3)
A 		41
B 8.5
(-1)
A 		9.2
(-0.6)
A 		5.2
(-3)
A 		15.3
(3)
A 		10
C 7.4
(-0.5)
A 		11.1
(3)
A 		4.7
(-3)
A 		9.7
(1.7)
A 		6
avr 8.4
(-2.3) 		8.5
(-2.2) 		6.3
(-3) 		23.8
(3) 		18

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 9
1 2 3 4 5 6 range
A 12.5
(3)
A 		6.2
(-1.8)
A 		4.8
(-2.8)
A 		4.6
(-3)
A 		5.7
(-2.2)
A 		7.2
(-1)
A 		8
B 7
(3)
A 		4.3
(-2.2)
A 		4.5
(-1.8)
A 		6.6
(2.2)
A 		4.7
(-1.5)
A 		3.9
(-3)
A 		3
C 4.2
(-3)
A 		7.9
(3)
A 		6.3
(0.4)
A 		4.6
(-2.4)
A 		5.6
(-0.7)
A 		5.2
(-1.4)
A 		4
avr 7.9
(3) 		6.1
(-1) 		5.2
(-3) 		5.3
(-2.8) 		5.3
(-2.8) 		5.4
(-2.6) 		3
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 6
1 2 3 4 range
A 3.4
(-0.6)
A 		1.1
(-3)
A 		2.3
(-1.7)
A 		6.8
(3)
A 		6
B 2.7
(1.3)
A 		2.3
(0.1)
A 		1.2
(-3)
A 		3.3
(3)
A 		2
C 2.5
(0.4)
A 		3.2
(3)
A 		1.7
(-2.6)
A 		1.6
(-3)
A 		2
avr 2.9
(0.3) 		2.2
(-1.6) 		1.7
(-3) 		3.9
(3) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2
1 2 3 4 5 6 range
A 2.3
(3)
A 		2.2
(2.5)
A 		1
(-3)
A 		1.2
(-2.1)
A 		1.7
(0.2)
A 		1.7
(0.2)
A 		1
B 2.4
(3)
A 		1.4
(-1.6)
A 		1.1
(-3)
A 		1.7
(-0.2)
A 		1.9
(0.7)
A 		1.5
(-1.2)
A 		1
C 1.5
(-0.6)
A 		2.4
(3)
A 		1.6
(-0.2)
A 		0.9
(-3)
A 		1.8
(0.6)
A 		1.8
(0.6)
A 		2
avr 2.1
(3) 		2
(2.3) 		1.2
(-3) 		1.3
(-2.3) 		1.8
(1) 		1.7
(0.3) 		1
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178157_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12972
Sequence Type Exemplar sequence
Representative Public ID F53F8.5 NCBI
Target Description F53F8.5 /REP_DB=WormBase Gene ID /WP=CE17859 /TR=O62258 /GB=CAB04458.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075597(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075597 NCBI Caenorhabditis elegans F53F8.5 (F53F8.5) mRNA, complete cds. 11/11 None
SNAP00000039379 ENSEMBL cdna:SNAP chromosome:CEL140:V:20698610:20702088:-1 11/11 None
GENEFINDER00000039382 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:20698009:20702088:-1 11/11 None
F53F8.5 ENSEMBL cdna:known chromosome:CEL140:V:20698610:20702088:-1 gene:F53F8.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:20698609-20702088(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F53F8.5
Entrez Gene 186182 Entrez gene
SwissProt O62258 EMBL-EBI
WormBase CE17859 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04458 Hypothetical protein F53F8.5 [Caenorhabditis elegans] ref|NP_507998.1| F53F8.5 [Caenorhabditis elegans] 1.0E-125
blast CAB04458 Hypothetical protein F53F8.5 [Caenorhabditis elegans] ref|NP_507998.1| F53F8.5 [Caenorhabditis elegans] 1.0E-122
blast CAE61682 Hypothetical protein CBG05626 [Caenorhabditis briggsae] 7.0E-70
blast CAE61682 Hypothetical protein CBG05626 [Caenorhabditis briggsae] 2.0E-66
blast AAX27881 SJCHGC04591 protein [Schistosoma japonicum] 4.0E-18
Protein Domains
Database ID Description E-Value
Pfam IPR001660 EMBL-EBI Sterile alpha motif SAM 3.9E-9
Pfam IPR001660 EMBL-EBI Sterile alpha motif SAM 1.2E-5
Sequence
Target Sequence 
>C. ELEGANS:178157_AT
ctgatcgccggcatatttcgtcgaaaatctccagaatttctcaatcgggcattaaaaaaa
caatttcgaatcgtccgccattgaattcccgtctcacacagtcccccgccgccgccacgt
catcacttcgaatccacgtgggacccaacggacggagcaaagcctcgcggatggacggac
ggcttcagaaagtttcaactgccgcgtcttccagtgatgttctgcttatagacgataata
tggattatgaagacgatgagattgtggaggaagttgttgattatgaggaggatgagatgg
gctattcggatgctccgatctacgacgttgtcgatgctccagttcgtcagcgaatctcat
attcttctggccgcctgtcgagtacagttcaacgtgctccaccacgagcttctgctcctt
cttcatcgtctcgctccgcccatcaccaccaccagccacgtcatcatcgacacgacacgt
catcaagaggcggagctc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTGATCGCCGGCATATTTCGTCGAA 264 239 569 Antisense
TTCGAATCGTCCGCCATTGAATTCC 110 693 633 Antisense
GAATCCACGTGGGACCCAACGGACG 500 101 698 Antisense
AAAGCCTCGCGGATGGACGGACGGC 145 125 727 Antisense
GCGTCTTCCAGTGATGTTCTGCTTA 682 289 772 Antisense
TGCTCCGATCTACGACGTTGTCGAT 193 581 879 Antisense
ATGCTCCAGTTCGTCAGCGAATCTC 120 43 902 Antisense
AGCGAATCTCATATTCTTCTGGCCG 295 81 917 Antisense
CGAGTACAGTTCAACGTGCTCCACC 652 249 947 Antisense
CACGTCATCATCGACACGACACGTC 245 197 1025 Antisense
GACACGTCATCAAGAGGCGGAGCTC 490 367 1042 Antisense
Sequence Source Affymetrix Proprietary Database