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[178139_at]

Description T20B3.3 /REP_DB=WormBase Gene ID /WP=CE20088 /TR=Q9XUN5 /GB=CAB04741.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 25      4grp_total > 33      6grp_cgc > 25      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 95
1 2 3 4 range
A 36.3
(-3)
A 		72.9
(-0.3)
A 		86
(0.7)
A 		117
(3)
A 		81
B 42.3
(-2.3)
A 		56.5
(0.4)
A 		69.8
(3)
A 		38.7
(-3)
A 		31
C 22
(-3)
A 		74.6
(3)
P 		46.8
(-0.2)
A 		64.6
(1.9)
P 		53
avr 33.5
(-3) 		68
(2.2) 		67.5
(2.1) 		73.4
(3) 		40

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 84
1 2 3 4 5 6 range
A 51.9
(2.1)
A 		33.4
(0)
A 		6.6
(-3)
A 		59.5
(3)
P 		38.8
(0.7)
P 		15.6
(-2)
A 		53
B 49.2
(-0.1)
P 		37.4
(-1.1)
P 		84.1
(3)
P 		25.9
(-2.1)
A 		16.2
(-3)
A 		39.8
(-0.9)
A 		68
C 30
(-1.1)
A 		87.6
(3)
P 		10.5
(-2.5)
A 		26
(-1.4)
P 		4.1
(-3)
A 		32.3
(-1)
A 		84
avr 43.7
(1.4) 		52.8
(3) 		33.7
(-0.5) 		37.1
(0.2) 		19.7
(-3) 		29.2
(-1.3) 		33
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 15
1 2 3 4 range
A 13.2
(-3)
A 		15.8
(-1.3)
A 		22.5
(3)
A 		17.1
(-0.5)
A 		9
B 13.5
(1)
A 		14
(1.4)
A 		16.2
(3)
A 		8.2
(-3)
A 		8
C 7.3
(-3)
A 		21.4
(3)
P 		16.3
(0.8)
A 		10.9
(-1.5)
P 		14
avr 11.3
(-3) 		17.1
(2) 		18.3
(3) 		12.1
(-2.3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 25
1 2 3 4 5 6 range
A 9.6
(0.6)
A 		11.9
(1.6)
A 		1.4
(-3)
A 		15.1
(3)
P 		11.7
(1.5)
P 		3.8
(-1.9)
A 		14
B 17.1
(1.6)
P 		12.5
(-0.4)
P 		20.4
(3)
P 		6.9
(-2.9)
A 		6.6
(-3)
A 		14.8
(0.6)
A 		14
C 11.1
(-0.7)
A 		26.7
(3)
P 		2.7
(-2.7)
A 		5.2
(-2.1)
P 		1.3
(-3)
A 		10.8
(-0.8)
A 		25
avr 12.6
(0.5) 		17
(3) 		8.2
(-2) 		9.1
(-1.5) 		6.5
(-3) 		9.8
(-1.1) 		11
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178139_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13961
Sequence Type Exemplar sequence
Representative Public ID T20B3.3 NCBI
Target Description T20B3.3 /REP_DB=WormBase Gene ID /WP=CE20088 /TR=Q9XUN5 /GB=CAB04741.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074847(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074847 NCBI Caenorhabditis elegans Serpentine Receptor, class H family member (srh-215) (srh-215) mRNA, complete cds. 11/11 None
SNAP00000028348 ENSEMBL cdna:SNAP chromosome:CEL140:V:16833138:16834359:-1 11/11 None
GENEFINDER00000028358 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:16830816:16834602:-1 10/11 None
T20B3.3 ENSEMBL cdna:known chromosome:CEL140:V:16833138:16834602:-1 gene:T20B3.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:16833137-16834602(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol srh-215
Ensembl T20B3.3
Entrez Gene 188620 Entrez gene
SwissProt Q9XUN5 EMBL-EBI
WormBase CE20088 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6355 regulation of transcription, DNA-dependent inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3677 DNA binding inferred from electronic annotation QuickGO AmiGO
3700 transcription factor activity inferred from electronic annotation QuickGO AmiGO
4930 G-protein coupled receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB04741 Hypothetical protein T20B3.3 [Caenorhabditis elegans] ref|NP_507248.1| Serpentine Receptor, class H family member (srh-215) [Caenorhabditis elegans] 1.0E-180
blast CAB04741 Hypothetical protein T20B3.3 [Caenorhabditis elegans] ref|NP_507248.1| Serpentine Receptor, class H family member (srh-215) [Caenorhabditis elegans] 1.0E-164
blast CAB04741 Hypothetical protein T20B3.3 [Caenorhabditis elegans] ref|NP_507248.1| Serpentine Receptor, class H family member (srh-215) [Caenorhabditis elegans] 1.0E-130
blast CAB04743 Hypothetical protein T20B3.5 [Caenorhabditis elegans] ref|NP_507246.1| Serpentine Receptor, class H family member (srh-216) [Caenorhabditis elegans] 1.0E-129
blast CAB04742 Hypothetical protein T20B3.4 [Caenorhabditis elegans] ref|NP_507247.1| Serpentine Receptor, class H family member (srh-214) [Caenorhabditis elegans] 1.0E-124
blast CAB04742 Hypothetical protein T20B3.4 [Caenorhabditis elegans] ref|NP_507247.1| Serpentine Receptor, class H family member (srh-214) [Caenorhabditis elegans] 1.0E-120
blast CAB04743 Hypothetical protein T20B3.5 [Caenorhabditis elegans] ref|NP_507246.1| Serpentine Receptor, class H family member (srh-216) [Caenorhabditis elegans] 1.0E-119
Protein Domains
Database ID Description E-Value
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.0E-126
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 4.9E-108
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 2.5E-125
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_507248.1 7 20-42,63-85,100-119,140-159,193-215,244-266,276-298
T20B3.3 7 20-42,63-85,100-119,140-159,193-215,244-266,276-298
SNAP00000028348 5 20-42,55-77,112-134,163-185,195-217
GENEFINDER00000028358 13 20-42,63-85,100-119,140-159,193-215,244-266,276-298,311-333,343-363,384-403,437-459,486-508,523-542
Sequence
Target Sequence 
>C. ELEGANS:178139_AT
ccaacaagaaccttcatcgctacgccactccgatctaccttatccattatataataccac
taatagttattccaactgtcatcaaaatccccgatcaggaagagggtaagaagaacttca
tcaatttatttgaatgtgtacctccgtatgttgaactgaagaatgtattctatttgatcc
ttgtcaagaaattctttcttataagttgcggaattttcataatggttttgttcttggaag
tttggttcttggcatatatgactgatcggaagctaaagcagcagatgacaaagacaatgt
cccagaagactgtagacttgcatcgcaagtttcaaagagcattcatcttgcagctcctca
tcccattcctgatcgtcttcgttccacttggctacatcgggttcaccgcgatctttgact
atcacaatcagtttttcaataacttgacattaattataatatcatctcatggattcttct
caacgattgcaatggtagctcttcatgctccctaccgggaatatacattgaagttatttc
cattttttaaacgtctgagctcctcgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCAACAAGAACCTTCATCGCTACGC 126 277 398 Antisense
CTCCGATCTACCTTATCCATTATAT 241 235 425 Antisense
TGTCATCAAAATCCCCGATCAGGAA 261 557 474 Antisense
GAATGTGTACCTCCGTATGTTGAAC 31 331 529 Antisense
GAATGTATTCTATTTGATCCTTGTC 7 333 558 Antisense
GAAGACTGTAGACTTGCATCGCAAG 649 339 702 Antisense
AAGAGCATTCATCTTGCAGCTCCTC 563 153 732 Antisense
TCACCGCGATCTTTGACTATCACAA 113 627 800 Antisense
TGCAATGGTAGCTCTTCATGCTCCC 334 577 885 Antisense
TCTTCATGCTCCCTACCGGGAATAT 182 617 897 Antisense
TTTTTTAAACGTCTGAGCTCCTCGC 479 675 940 Antisense
Sequence Source Affymetrix Proprietary Database