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[178133_at]

Description E03H4.4 /REP_DB=WormBase Gene ID /WP=CE09131 /TR=O17744 /GB=CAB04026.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 37      4grp_total > 26      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 95
1 2 3 4 range
A 65
(-2)
A 		68.5
(-1.7)
A 		50.7
(-3)
A 		132.6
(3)
P 		82
B 47.4
(-3)
A 		76.3
(3)
A 		64.8
(0.6)
A 		68.2
(1.3)
A 		29
C 37.3
(-3)
A 		69.6
(2.5)
A 		43.4
(-2)
A 		72.7
(3)
A 		35
avr 49.9
(-3) 		71.5
(0.1) 		53
(-2.5) 		91.2
(3) 		41

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 63
1 2 3 4 5 6 range
A 86.4
(3)
A 		44.1
(-2.3)
A 		59.1
(-0.4)
A 		42.2
(-2.5)
A 		38.4
(-3)
A 		64.5
(0.3)
A 		48
B 54.6
(0.4)
A 		49.3
(-0.6)
A 		37.3
(-3)
A 		41.4
(-2.2)
A 		38.6
(-2.7)
A 		67.7
(3)
A 		30
C 45.5
(3)
P 		25.7
(-2.4)
A 		36.7
(0.6)
A 		41.1
(1.8)
A 		23.6
(-3)
A 		23.5
(-3)
A 		22
avr 62.2
(3) 		39.7
(-1.7) 		44.4
(-0.7) 		41.6
(-1.3) 		33.5
(-3) 		51.9
(0.8) 		29
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 11
1 2 3 4 range
A 23.7
(3)
A 		14.9
(-2)
A 		13.2
(-3)
A 		19.4
(0.5)
P 		11
B 15.1
(-2.2)
A 		18.8
(3)
A 		15
(-2.3)
A 		14.5
(-3)
A 		4
C 12.3
(-3)
A 		20
(3)
A 		15.1
(-0.8)
A 		12.3
(-3)
A 		8
avr 17
(1.5) 		17.9
(3) 		14.4
(-3) 		15.4
(-1.3) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 18
1 2 3 4 5 6 range
A 15.9
(3)
A 		15.7
(2.8)
A 		12.9
(-0.5)
A 		10.7
(-3)
A 		11.6
(-2)
A 		15.7
(2.8)
A 		5
B 19
(0.7)
A 		16.4
(-0.3)
A 		9.1
(-3)
A 		11
(-2.3)
A 		15.7
(-0.6)
A 		25.3
(3)
A 		16
C 16.9
(3)
P 		7.9
(-2.8)
A 		9.3
(-1.9)
A 		8.3
(-2.5)
A 		7.6
(-3)
A 		7.9
(-2.8)
A 		9
avr 17.3
(3) 		13.3
(-0.3) 		10.4
(-2.7) 		10
(-3) 		11.6
(-1.7) 		16.3
(2.2) 		7
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178133_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2489
Sequence Type Exemplar sequence
Representative Public ID E03H4.4 NCBI
Target Description E03H4.4 /REP_DB=WormBase Gene ID /WP=CE09131 /TR=O17744 /GB=CAB04026.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060746(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060746 NCBI Caenorhabditis elegans E03H4.4 (E03H4.4) mRNA, complete cds. 11/11 None
SNAP00000031037 ENSEMBL cdna:SNAP chromosome:CEL140:I:12415408:12419556:1 11/11 None
GENEFINDER00000031049 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:12415408:12419556:1 11/11 None
E03H4.4 ENSEMBL cdna:known chromosome:CEL140:I:12415685:12419408:1 gene:E03H4.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:12415615-12419339(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl E03H4.4
Entrez Gene 184019 Entrez gene
SwissProt O17744 EMBL-EBI
WormBase CE38312 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04026 Hypothetical protein E03H4.4 [Caenorhabditis elegans] ref|NP_493147.2| E03H4.4 [Caenorhabditis elegans] 0.0
blast CAB04496 Hypothetical protein F56H6.7 [Caenorhabditis elegans] ref|NP_493108.2| F56H6.7 [Caenorhabditis elegans] 0.0
blast CAB04498 Hypothetical protein F56H6.9 [Caenorhabditis elegans] ref|NP_493110.2| F56H6.9 [Caenorhabditis elegans] 0.0
blast NP_503697 Y45G12C.11 [Caenorhabditis elegans] gb|AAF60585.2| Hypothetical protein Y45G12C.11 [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR005049 EMBL-EBI Protein of unknown function DUF288 1.1E-86
Pfam IPR005049 EMBL-EBI Protein of unknown function DUF288 1.0E-126
Pfam IPR005049 EMBL-EBI Protein of unknown function DUF288 1.0E-126
Pfam IPR005049 EMBL-EBI Protein of unknown function DUF288 1.0E-126
Pfam IPR005049 EMBL-EBI Protein of unknown function DUF288 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_493147.2 1 5-24
E03H4.4 1 5-24
Sequence
Target Sequence 
>C. ELEGANS:178133_AT
tttcctatggagcctatgcctaccactgcgttactctcgtcaaagaaatgcgtttgaata
atgtgcaaggatattttatgatgtctgacgatgctgtctttaatatatggcagcgtgtcg
actactcgagagtacatcaccttacaggatctctaaatatttacaatacgtattttacag
gtatatatggcgaaccagctgccaaaaaaatattagccactgtgaaggactcaacagact
taaaagttttggaaacgtggaaaaagttcgacgatggtttgaaaaagtacgcatataaca
acattacggatggggcagaagccgtacttttgagcacttacgggaaaagttactcggact
tctattacattccttcaactgaaattgattactatgccgacttgatgagactcttctatg
atggtggactgtttctagagactgctgttgatagattcataagatctattaatcatcaaa
catcactcgccggggaatccagttatctttggtttgacgatcgtgagaaatggccaaagc
tctataataagaacctcgttgctctgcatccagtcaagcctagcatgttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTTCCTATGGAGCCTATGCCTACCA 707 667 1517 Antisense
CACTGCGTTACTCTCGTCAAAGAAA 200 199 1540 Antisense
TATATGGCAGCGTGTCGACTACTCG 480 659 1620 Antisense
GAGTACATCACCTTACAGGATCTCT 244 399 1646 Antisense
GGGCAGAAGCCGTACTTTTGAGCAC 450 493 1829 Antisense
GTTACTCGGACTTCTATTACATTCC 280 447 1865 Antisense
ATGCCGACTTGATGAGACTCTTCTA 138 41 1910 Antisense
GGTGGACTGTTTCTAGAGACTGCTG 484 499 1939 Antisense
ATCACTCGCCGGGGAATCCAGTTAT 521 23 1998 Antisense
AATAAGAACCTCGTTGCTCTGCATC 652 171 2062 Antisense
GCATCCAGTCAAGCCTAGCATGTTC 460 309 2082 Antisense
Sequence Source Affymetrix Proprietary Database