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[178128_at]

Description F01D5.3 /REP_DB=WormBase Gene ID /WP=CE18561 /TR=Q9XVB4 /GB=CAB04036.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 11      6grp_high1_10_10 > 07      4grp_high_8_8 > 51      6grp_highest_nonorm > 80      6grp_total > 04      4grp_total > 60      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 463
1 2 3 4 range
A 468.6
(3)
P 		360.3
(1.2)
P 		387.9
(1.6)
P 		113.7
(-3)
P 		355
B 412.1
(2.3)
P 		431.5
(2.6)
P 		459.7
(3)
P 		39
(-3)
A 		421
C 493.5
(2.9)
P 		501.7
(3)
P 		478.6
(2.7)
P 		49.3
(-3)
A 		452
avr 458.1
(3) 		431.2
(2.6) 		442.1
(2.8) 		67.3
(-3) 		391

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 362
1 2 3 4 5 6 range
A 338.3
(3)
P 		267.8
(1.3)
P 		154.5
(-1.4)
P 		237.8
(0.6)
P 		144.2
(-1.7)
P 		90.3
(-3)
P 		248
B 427.7
(3)
P 		287.1
(0.6)
P 		249.9
(0)
P 		246
(-0)
P 		145.1
(-1.7)
P 		69
(-3)
P 		359
C 431.3
(3)
P 		277.2
(0.2)
P 		162.4
(-1.8)
P 		256
(-0.1)
P 		165.8
(-1.8)
P 		96.4
(-3)
P 		335
avr 399.1
(3) 		277.4
(0.7) 		188.9
(-1) 		246.6
(0.1) 		151.7
(-1.7) 		85.2
(-3) 		314
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 162
1 2 3 4 range
A 170.6
(3)
P 		78.2
(-0.6)
P 		101.4
(0.3)
P 		16.6
(-3)
P 		154
B 131.4
(3)
P 		106.6
(1.8)
P 		106.5
(1.8)
P 		8.3
(-3)
A 		123
C 163.4
(2.9)
P 		144.3
(2.1)
P 		166.8
(3)
P 		8.3
(-3)
A 		159
avr 155.1
(3) 		109.7
(1.1) 		124.9
(1.7) 		11.1
(-3) 		144

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 138
1 2 3 4 5 6 range
A 62.4
(0.3)
P 		95.3
(3)
P 		33.7
(-2)
P 		60.3
(0.1)
P 		43.5
(-1.2)
P 		22
(-3)
P 		73
B 148.8
(3)
P 		95.6
(0.4)
P 		60.7
(-1.3)
P 		65.2
(-1.1)
P 		59
(-1.4)
P 		25.7
(-3)
P 		123
C 159.9
(3)
P 		84.6
(-0.5)
P 		41.3
(-2.6)
P 		51.5
(-2.1)
P 		53.6
(-2)
P 		32.3
(-3)
P 		128
avr 123.7
(3) 		91.8
(1) 		45.2
(-1.9) 		59
(-1) 		52
(-1.4) 		26.7
(-3) 		97
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178128_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3106
Sequence Type Exemplar sequence
Representative Public ID F01D5.3 NCBI
Target Description F01D5.3 /REP_DB=WormBase Gene ID /WP=CE18561 /TR=Q9XVB4 /GB=CAB04036.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064533(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064533 NCBI Caenorhabditis elegans F01D5.3 (F01D5.3) mRNA, complete cds. 11/11 None
F01D5.3 ENSEMBL cdna:known chromosome:CEL140:II:13999098:13999921:-1 gene:F01D5.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000039847 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:13999094-13999918(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F01D5.3
Entrez Gene 184057 Entrez gene
SwissProt Q9XVB4 EMBL-EBI
WormBase CE18561 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
4872 receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB04036 Hypothetical protein F01D5.3 [Caenorhabditis elegans] ref|NP_496934.1| F01D5.3 [Caenorhabditis elegans] 1.0E-71
blast CAB04040 Hypothetical protein F01D5.2 [Caenorhabditis elegans] ref|NP_496933.1| F01D5.2 [Caenorhabditis elegans] 6.0E-55
blast CAE73378 Hypothetical protein CBG20818 [Caenorhabditis briggsae] 4.0E-49
Protein Domains
Database ID Description E-Value
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.7E-15
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 5.1E-13
Sequence
Target Sequence 
>C. ELEGANS:178128_AT
ctactccaaagctctattctcttgctttccgtctcccttgcagcgtgccaaatttccact
gcaccatgccaagatgacccgtatacggattgcagggactacgcggctctttgcaataat
ccaatgtacaaaaattttctagatgttttttgtccaaaaacttgtgggctttgctcggat
tcgactactttagttccgccgacagggagctcgaattgtgtggatacaaatgttcattgc
aaatcatgggtgaagcagggttactgtacttcttgctttgtggattgtgctgaaagaatt
caaaactgtgcaaaatcatgtggcttctgcaacccgggagcctgcataaattgcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTACTCCAAAGCTCTATTCTCTTGC 584 225 16 Antisense
CTTGCAGCGTGCCAAATTTCCACTG 679 217 52 Antisense
TTTCCACTGCACCATGCCAAGATGA 521 667 68 Antisense
ATGCCAAGATGACCCGTATACGGAT 79 41 81 Antisense
TACGGATTGCAGGGACTACGCGGCT 45 647 99 Antisense
CTACGCGGCTCTTTGCAATAATCCA 135 227 114 Antisense
AAAAACTTGTGGGCTTTGCTCGGAT 67 131 171 Antisense
ACTTTAGTTCCGCCGACAGGGAGCT 685 101 202 Antisense
CAGGGAGCTCGAATTGTGTGGATAC 293 207 218 Antisense
GTTACTGTACTTCTTGCTTTGTGGA 705 445 275 Antisense
AACCCGGGAGCCTGCATAAATTGCC 688 145 346 Antisense
Sequence Source Affymetrix Proprietary Database