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[178125_at]

Description C54C6.5 /REP_DB=WormBase Gene ID /WP=CE05496 /TR=Q18819 /GB=CAB00856.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 42      6grp_high1_10_10 > 24      4grp_high_8_8 > 25      6grp_highest_nonorm > 90      6grp_total > 25      4grp_total > 38      6grp_highest_sub > 02      6grp_high1_sub > 02      6grp_total_sub > Decrease      6grp_rule_based > S2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 300
1 2 3 4 range
A 304.4
(3)
P 		195.3
(-0.4)
P 		114
(-3)
P 		208.1
(-0)
M 		190
B 282.3
(3)
P 		127.9
(-2)
P 		95.2
(-3)
P 		115.1
(-2.4)
P 		187
C 395.3
(3)
P 		136.9
(-2.7)
P 		122.1
(-3)
P 		189.8
(-1.5)
P 		273
avr 327.3
(3) 		153.4
(-1.8) 		110.4
(-3) 		171
(-1.3) 		217

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 258
1 2 3 4 5 6 range
A 192.3
(2.1)
P 		124.9
(-1.3)
P 		210.2
(3)
P 		137.8
(-0.7)
P 		127.3
(-1.2)
P 		92.5
(-3)
P 		118
B 201.6
(-0.4)
P 		324.4
(3)
P 		148
(-1.9)
P 		107.9
(-3)
P 		128.9
(-2.4)
P 		119.9
(-2.7)
P 		217
C 205.9
(0.7)
P 		291.7
(3)
P 		118.8
(-1.6)
P 		98.3
(-2.1)
P 		66.2
(-3)
P 		107.3
(-1.9)
P 		226
avr 199.9
(1) 		247
(3) 		159
(-0.8) 		114.7
(-2.7) 		107.5
(-3) 		106.6
(-3) 		140
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 109
1 2 3 4 range
A 110.8
(3)
P 		42.4
(-2.1)
P 		29.8
(-3)
P 		30.5
(-2.9)
M 		81
B 90
(3)
P 		31.6
(-2.2)
P 		22.1
(-3)
P 		24.5
(-2.8)
P 		68
C 130.9
(3)
P 		39.4
(-2.6)
P 		42.6
(-2.4)
P 		32
(-3)
P 		99
avr 110.6
(3) 		37.8
(-2.4) 		31.5
(-2.8) 		29
(-3) 		82

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 88
1 2 3 4 5 6 range
A 35.5
(0.3)
P 		44.4
(2.6)
P 		45.8
(3)
P 		34.9
(0.2)
P 		38.4
(1.1)
P 		22.5
(-3)
P 		23
B 70.1
(0.1)
P 		108
(3)
P 		35.9
(-2.4)
P 		28.6
(-3)
P 		52.4
(-1.2)
P 		44.7
(-1.8)
P 		79
C 76.3
(1.9)
P 		89
(3)
P 		30.2
(-2.1)
P 		19.8
(-3)
P 		21.4
(-2.9)
P 		35.9
(-1.6)
P 		69
avr 60.6
(0.7) 		80.5
(3) 		37.3
(-1.9) 		27.8
(-3) 		37.4
(-1.9) 		34.4
(-2.2) 		53
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178125_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6163
Sequence Type Exemplar sequence
Representative Public ID C54C6.5 NCBI
Target Description C54C6.5 /REP_DB=WormBase Gene ID /WP=CE05496 /TR=Q18819 /GB=CAB00856.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065328(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065328 NCBI Caenorhabditis elegans C54C6.5 (C54C6.5) mRNA, complete cds. 11/11 None
C54C6.5 ENSEMBL cdna:known chromosome:CEL140:III:3549322:3549821:-1 gene:C54C6.5 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000035319 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:3549321-3549821(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C54C6.5
Entrez Gene 183767 Entrez gene
SwissProt Q18819 EMBL-EBI
WormBase CE05496 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB00856 Hypothetical protein C54C6.5 [Caenorhabditis elegans] ref|NP_497729.1| C54C6.5 [Caenorhabditis elegans] 6.0E-51
blast CAE64930 Hypothetical protein CBG09756 [Caenorhabditis briggsae] 4.0E-10
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_497729.1 3 5-22,73-95,116-137
C54C6.5 3 5-22,73-95,116-137
Sequence
Target Sequence 
>C. ELEGANS:178125_AT
aaatgctccattttcttggctcttgtgctcttctcagccgtcttgctcttccagtcagaa
gcctcctctgttccatcttctgaagtacagaatacattgttcaagccatcatctgttgtt
ttccgtgctaaatgcaaaatgatgtgctcacaaatgtgctctccggttttggtgagcaaa
aaaatgatgacgtgcattcatattagtatgaatattttcagggagtatgtgatcggtaca
acaaatgcgtttgtctcaataattatcgcattttttaaaattatgacggaacatattttt
ttgtccaaaatgacagagaacaaaaaaaccaaaaaaaaaaaaggttttttttttcaaaac
actaccactattaaaaag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAATGCTCCATTTTCTTGGCTCTTG 609 119 16 Antisense
CAAGCCATCATCTGTTGTTTTCCGT 542 185 117 Antisense
TCTGTTGTTTTCCGTGCTAAATGCA 136 609 127 Antisense
GCTAAATGCAAAATGATGTGCTCAC 287 303 142 Antisense
CTCACAAATGTGCTCTCCGGTTTTG 700 225 162 Antisense
TGTGCTCTCCGGTTTTGGTGAGCAA 123 557 170 Antisense
GATGACGTGCATTCATATTAGTATG 286 411 201 Antisense
CAGGGAGTATGTGATCGGTACAACA 9 207 234 Antisense
GGTACAACAAATGCGTTTGTCTCAA 429 503 250 Antisense
GTCTCAATAATTATCGCATTTTTTA 669 469 268 Antisense
TCAAAACACTACCACTATTAAAAAG 244 629 369 Antisense
Sequence Source Affymetrix Proprietary Database