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[178112_at]

Description F10B5.7 /REP_DB=WormBase Gene ID /WP=CE02178 /TR=Q19285 /GB=CAA91312.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 54      6grp_high1_10_10 > 44      4grp_highest_8_8 > 59      4grp_high_8_8 > 25      6grp_highest_nonorm > 81      6grp_total > 30      4grp_total > 38      6grp_cgc > 72      6grp_high1_sub > 02      6grp_cgc_sub > 02      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 220
1 2 3 4 range
A 347.4
(3)
P 		174
(-1.7)
P 		127.1
(-3)
P 		205.7
(-0.9)
P 		220
B 285.6
(3)
P 		188.7
(-0.7)
P 		128.4
(-3)
P 		162.1
(-1.7)
P 		157
C 292.9
(3)
P 		173.7
(-2.4)
P 		159.7
(-3)
P 		175.9
(-2.3)
P 		133
avr 308.6
(3) 		178.8
(-1.6) 		138.4
(-3) 		181.2
(-1.5) 		170

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 180
1 2 3 4 5 6 range
A 224.4
(-1.6)
P 		294
(1.5)
P 		316.4
(2.6)
P 		326.2
(3)
P 		193.6
(-3)
P 		265.4
(0.2)
P 		133
B 363.7
(2.6)
P 		373.8
(3)
P 		287.1
(-0.6)
P 		308.7
(0.3)
P 		231
(-3)
P 		320.6
(0.8)
P 		143
C 242.5
(-3)
P 		370.9
(3)
P 		312.5
(0.3)
P 		286.7
(-0.9)
P 		258.4
(-2.3)
P 		284.8
(-1)
P 		128
avr 276.9
(-0.5) 		346.2
(3) 		305.3
(0.9) 		307.2
(1) 		227.7
(-3) 		290.3
(0.2) 		119
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 97
1 2 3 4 range
A 126.5
(3)
P 		37.8
(-2.5)
P 		33.2
(-2.8)
P 		30.1
(-3)
P 		96
B 91.1
(3)
P 		46.6
(-1.4)
P 		29.8
(-3)
P 		34.5
(-2.5)
P 		61
C 96.9
(3)
P 		49.9
(-1.2)
P 		55.7
(-0.7)
P 		29.7
(-3)
P 		67
avr 104.8
(3) 		44.8
(-1.9) 		39.6
(-2.3) 		31.4
(-3) 		73

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 85
1 2 3 4 5 6 range
A 41.4
(-3)
P 		104.5
(3)
P 		68.9
(-0.4)
P 		82.7
(0.9)
P 		58.4
(-1.4)
P 		64.6
(-0.8)
P 		63
B 126.5
(3)
P 		124.4
(2.8)
P 		69.7
(-3)
P 		81.8
(-1.7)
P 		93.8
(-0.5)
P 		119.5
(2.3)
P 		57
C 89.9
(0.5)
P 		113.2
(3)
P 		79.5
(-0.6)
P 		57.7
(-3)
P 		83.6
(-0.2)
P 		95.3
(1.1)
P 		56
avr 85.9
(-1.1) 		114
(3) 		72.7
(-3) 		74.1
(-2.8) 		78.6
(-2.1) 		93.1
(-0) 		41
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178112_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2687
Sequence Type Exemplar sequence
Representative Public ID F10B5.7 NCBI
Target Description F10B5.7 /REP_DB=WormBase Gene ID /WP=CE02178 /TR=Q19285 /GB=CAA91312.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063312(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063312 NCBI Caenorhabditis elegans RNA-dependent RNA polymerase Family family member (rrf-3) (rrf-3) mRNA, complete cds. 11/11 None
GENEFINDER00000014825 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:8162479:8168072:1 9/11 None
F10B5.7 ENSEMBL cdna:known chromosome:CEL140:II:8162479:8168474:1 gene:F10B5.7 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000002106 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:8162475-8168430(+) 99.31 100.0
Public Domain and Genome References
Gene Title
Gene Symbol rrf-3
Ensembl F10B5.7
Entrez Gene 174315 Entrez gene
SwissProt Q19285 EMBL-EBI
WormBase CE02178 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA91312 Hypothetical protein F10B5.7 [Caenorhabditis elegans] emb|CAA88315.1| Hypothetical protein F10B5.7 [Caenorhabditis elegans] ref|NP_495713.1| RNA-dependent RNA polymerase Family family member (rrf-3) [Caenorhabditis elegans] 0.0
blast CAE57720 Hypothetical protein CBG00730 [Caenorhabditis briggsae] 0.0
blast CAB01701 Hypothetical protein F26A3.3 [Caenorhabditis elegans] ref|NP_492132.1| Enhancer of Glp-One (glp-1) family member (ego-1) [Caenorhabditis elegans] gb|AAF80367.1| RNA-directed RNA polymerase related EGO-1 [Caenorhabditis elegans] 1.0E-147
blast CAE57375 Hypothetical protein CBG00321 [Caenorhabditis briggsae] 1.0E-146
Protein Domains
Database ID Description E-Value
Pfam IPR007855 EMBL-EBI RNA dependent RNA polymerase 1.0E-126
Pfam IPR007855 EMBL-EBI RNA dependent RNA polymerase 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:178112_AT
gatgcagatgaagctccgatattgagaaatttcgatatgggaacaatgatgctcgaattt
ctcagatatttggcaagtcaatctttcgcatcagctgaaagcataagtcttcgagttttc
tacgaaaaagatattgttgagccaatacttactaaaagtgcacaatggatgcctttacat
cttattgcatatcgtacatttcattccatcgctgtgtctggacggtttgatgcgcttcat
ttggacgatgaggatgcggtggatcagattacagaaagcaaggatccaatactagttaac
gagtcattgttctcttcgaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATGCAGATGAAGCTCCGATATTGA 332 413 4783 Antisense
GCTCGAATTTCTCAGATATTTGGCA 417 297 4833 Antisense
TGGCAAGTCAATCTTTCGCATCAGC 329 545 4853 Antisense
AAGTGCACAATGGATGCCTTTACAT 379 159 4938 Antisense
GCCTTTACATCTTATTGCATATCGT 508 281 4953 Antisense
TGCATATCGTACATTTCATTCCATC 519 579 4968 Antisense
TCATTCCATCGCTGTGTCTGGACGG 335 619 4983 Antisense
TGTCTGGACGGTTTGATGCGCTTCA 185 559 4997 Antisense
GATGCGCTTCATTTGGACGATGAGG 535 413 5011 Antisense
GAGGATGCGGTGGATCAGATTACAG 628 403 5032 Antisense
TAACGAGTCATTGTTCTCTTCGAGA 205 639 5079 Antisense
Sequence Source Affymetrix Proprietary Database