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[178105_at]

Description M04D8.5 /REP_DB=WormBase Gene ID /WP=CE00623 /TR=Q21510 /GB=CAA83613.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 32      4grp_total > 05      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 99
1 2 3 4 range
A 65.8
(-0.7)
P 		44
(-3)
A 		49.7
(-2.4)
A 		101.7
(3)
P 		58
B 74
(2.1)
P 		47.6
(-2.6)
P 		45.4
(-3)
A 		79.1
(3)
P 		34
C 69.4
(-1.1)
P 		50.1
(-2.2)
P 		37.4
(-3)
M 		136.6
(3)
P 		99
avr 69.7
(-0.5) 		47.2
(-2.7) 		44.2
(-3) 		105.8
(3) 		62

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 73
1 2 3 4 5 6 range
A 50.5
(0.3)
P 		30.3
(-1.6)
A 		78.3
(3)
P 		46.1
(-0.1)
A 		58
(1.1)
P 		15.5
(-3)
A 		63
B 56.9
(3)
P 		42.2
(0.4)
P 		50.6
(1.9)
P 		22.7
(-3)
A 		50.3
(1.8)
P 		37.3
(-0.4)
P 		34
C 28.2
(-0.9)
A 		71.2
(3)
P 		31
(-0.7)
A 		50.7
(1.1)
A 		28.5
(-0.9)
A 		5.5
(-3)
A 		66
avr 45.2
(1.6) 		47.9
(2) 		53.3
(3) 		39.8
(0.6) 		45.6
(1.6) 		19.4
(-3) 		34
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 14
1 2 3 4 range
A 24
(3)
P 		9.6
(-3)
A 		13
(-1.6)
A 		14.9
(-0.8)
P 		14
B 23.6
(3)
P 		11.8
(-2.4)
P 		10.5
(-3)
A 		16.8
(-0.1)
P 		13
C 23
(3)
P 		14.4
(-2.2)
P 		13
(-3)
M 		23
(3)
P 		10
avr 23.5
(3) 		11.9
(-3) 		12.2
(-2.8) 		18.2
(0.3) 		12

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 20
1 2 3 4 5 6 range
A 9.3
(-0.6)
P 		10.8
(0.1)
A 		17
(2.8)
P 		11.7
(0.5)
A 		17.5
(3)
P 		3.8
(-3)
A 		14
B 19.8
(2.8)
P 		14
(0.3)
P 		12.3
(-0.4)
P 		6
(-3)
A 		20.4
(3)
P 		13.9
(0.3)
P 		14
C 10.5
(-0.4)
A 		21.7
(3)
P 		7.9
(-1.2)
A 		10.2
(-0.5)
A 		9.2
(-0.8)
A 		1.9
(-3)
A 		20
avr 13.2
(1.4) 		15.5
(2.9) 		12.4
(0.8) 		9.3
(-1.2) 		15.7
(3) 		6.5
(-3) 		9
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178105_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6050
Sequence Type Exemplar sequence
Representative Public ID M04D8.5 NCBI
Target Description M04D8.5 /REP_DB=WormBase Gene ID /WP=CE00623 /TR=Q21510 /GB=CAA83613.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066825(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066825 NCBI Caenorhabditis elegans M04D8.5 (M04D8.5) mRNA, complete cds. 11/11 None
GENEFINDER00000022949 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:10032235:10033204:-1 11/11 None
M04D8.5 ENSEMBL cdna:known chromosome:CEL140:III:10032235:10033204:-1 gene:M04D8.5 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000022945 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:10032230-10033200(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl M04D8.5
Entrez Gene 187444 Entrez gene
SwissProt Q21510 EMBL-EBI
WormBase CE00623 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA83613 Hypothetical protein M04D8.5 [Caenorhabditis elegans] ref|NP_499226.1| M04D8.5 [Caenorhabditis elegans] 1.0E-106
blast CAE65064 Hypothetical protein CBG09912 [Caenorhabditis briggsae] 7.0E-28
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_499226.1 3 20-42,47-69,86-108
M04D8.5 3 20-42,47-69,86-108
GENEFINDER00000022949 3 20-42,47-69,86-108
Sequence
Target Sequence 
>C. ELEGANS:178105_AT
ttttttgtcctgcgtttctatcgctctcaacaatctctcatactcgtgctctaattctgg
catttgatttccaatgtcttgcagttttctttttgatgtttctaattgatccaaatcccc
atctgagaatctatttcatgttttacatttctctatttacacttccaactgcatacgttg
tggttttcggtgatcgaatttggatgagagtggcctttgtatgtcagctaacgagaatgg
tattcattggattccacaatgttttttacccaatttttgttgctgcatacattgcgattg
gctacgaaaaccatcacccgggaactacttcagatgagattctcatgcattcaacttcct
atggggaacctgcaaaacttttccaaatctaccgccttcaaatgatgctcaaaacaactc
cagatgtttcagtcgacgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTTTTTGTCCTGCGTTTCTATCGCT 549 675 32 Antisense
TCTCATACTCGTGCTCTAATTCTGG 455 613 67 Antisense
GATCCAAATCCCCATCTGAGAATCT 573 415 139 Antisense
CATTTCTCTATTTACACTTCCAACT 435 215 177 Antisense
GCATACGTTGTGGTTTTCGGTGATC 46 309 202 Antisense
GAGTGGCCTTTGTATGTCAGCTAAC 623 401 239 Antisense
GATTGGCTACGAAAACCATCACCCG 552 435 327 Antisense
ATCACCCGGGAACTACTTCAGATGA 643 23 344 Antisense
GATTCTCATGCATTCAACTTCCTAT 393 431 369 Antisense
TTCCAAATCTACCGCCTTCAAATGA 487 701 412 Antisense
CAACTCCAGATGTTTCAGTCGACGG 217 187 446 Antisense
Sequence Source Affymetrix Proprietary Database