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[178082_s_at]

Description R160.7 /REP_DB=WormBase Gene ID /WP=CE19557 /TR=Q9TZD0 /GB=AAC68738.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 82      6grp_high1_10_10 > 28      4grp_highest_8_8 > 31      4grp_high_8_8 > 12      6grp_highest_nonorm > 74      6grp_total > 27      4grp_total > 12      6grp_highest_sub > Cu      6grp_cgc > 04      6grp_high1_sub > De      6grp_cgc_sub > Cu      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 787
1 2 3 4 range
A 790
(-0.8)
P 		637.9
(-2)
P 		503.7
(-3)
P 		1291.1
(3)
P 		787
B 571
(-3)
P 		615.7
(-2.4)
P 		567
(-3)
P 		1064.4
(3)
P 		497
C 729.4
(-0.4)
P 		549.4
(-2.8)
P 		536.7
(-3)
P 		982.7
(3)
P 		446
avr 696.8
(-1.3) 		601
(-2.3) 		535.8
(-3) 		1112.7
(3) 		577

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 259
1 2 3 4 5 6 range
A 753.3
(3)
P 		646
(0.5)
P 		496.7
(-3)
P 		504.3
(-2.8)
P 		634.3
(0.2)
P 		654
(0.7)
P 		257
B 705.1
(3)
P 		651.9
(1.5)
P 		493.9
(-3)
P 		636.5
(1.1)
P 		522
(-2.2)
P 		612.3
(0.4)
P 		211
C 701
(3)
P 		676.2
(2.1)
P 		551.3
(-2.5)
P 		616.9
(-0.1)
P 		539.1
(-3)
P 		581.3
(-1.4)
P 		162
avr 719.8
(3) 		658
(1.2) 		514
(-3) 		585.9
(-0.9) 		565.1
(-1.5) 		615.9
(-0) 		206
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 156
1 2 3 4 range
A 287.6
(3)
P 		138.5
(-2.7)
P 		131.7
(-3)
P 		189
(-0.8)
P 		156
B 182.1
(0.2)
P 		152.1
(-1.7)
P 		131.4
(-3)
P 		226.8
(3)
P 		95
C 241.5
(3)
P 		158
(-3)
P 		187.1
(-0.9)
P 		165.7
(-2.4)
P 		84
avr 237.1
(3) 		149.5
(-3) 		150.1
(-3) 		193.8
(0) 		88

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 152
1 2 3 4 5 6 range
A 138.9
(-1.5)
P 		229.8
(3)
P 		108.2
(-3)
P 		127.9
(-2)
P 		191.4
(1.1)
P 		159.2
(-0.5)
P 		122
B 245.3
(3)
P 		217
(1.6)
P 		119.9
(-3)
P 		168.6
(-0.7)
P 		212.1
(1.4)
P 		228.3
(2.2)
P 		125
C 259.8
(3)
P 		206.3
(0.6)
P 		140.3
(-2.3)
P 		124.1
(-3)
P 		174.4
(-0.8)
P 		194.6
(0.1)
P 		136
avr 214.7
(2.8) 		217.7
(3) 		122.8
(-3) 		140.2
(-1.9) 		192.6
(1.4) 		194
(1.5) 		95
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 178082_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16941
Sequence Type Exemplar sequence
Representative Public ID R160.7 NCBI
Target Description R160.7 /REP_DB=WormBase Gene ID /WP=CE19557 /TR=Q9TZD0 /GB=AAC68738.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076355(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076355 NCBI Caenorhabditis elegans Lateral Signaling Target family member (lst-2) (lst-2) mRNA, complete cds. 11/11 None
SNAP00000044073 ENSEMBL cdna:SNAP chromosome:CEL140:X:4378610:4381661:-1 9/11 None
GENEFINDER00000044080 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:4378610:4383331:-1 9/11 None
R160.7 ENSEMBL cdna:known chromosome:CEL140:X:4377978:4383339:-1 gene:R160.7 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:4378288-4383332(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol lst-2
Ensembl R160.7
Entrez Gene 180712 Entrez gene
SwissProt Q9TZD0 EMBL-EBI
WormBase CE33815 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.22890.1.S1_AT similar to Zinc finger FYVE domain containing protein 28 cfa
CHICKEN:GGAAFFX.10003.1.S1_S_AT similar to mKIAA1643 protein gga
HG-U133_PLUS_2:232408_AT zinc finger, FYVE domain containing 28 hs
HG-U133B:232408_AT zinc finger, FYVE domain containing 28 hs
U133_X3P:HS.247383.0.S1_3P_AT zinc finger, FYVE domain containing 28 hs
HG-U133_PLUS_2:1569383_S_AT zinc finger, FYVE domain containing 28 hs
U133_X3P:HS2.388998.1.S1_3P_AT Zinc finger, FYVE domain containing 28 hs
U133_X3P:HS2.382606.1.S1_3P_S_AT Zinc finger, FYVE domain containing 28 hs
HG-U133_PLUS_2:1569369_AT Zinc finger, FYVE domain containing 28 hs
HG-U95C:50816_AT Zinc finger, FYVE domain containing 28 hs
HG-U95E:69901_AT Zinc finger, FYVE domain containing 28 hs
HG-U95E:79870_AT Zinc finger, FYVE domain containing 28 hs
MG-U74BV2:107366_AT zinc finger, FYVE domain containing 28 mm
MOE430B:1434504_AT zinc finger, FYVE domain containing 28 mm
MOUSE430_2:1434504_AT zinc finger, FYVE domain containing 28 mm
MG-U74BV2:111110_AT Zinc finger, FYVE domain containing 28 (Zfyve28), mRNA mm
MOUSE430_2:1443669_AT Zinc finger, FYVE domain containing 28 (Zfyve28), mRNA mm
MOE430B:1443669_AT Zinc finger, FYVE domain containing 28 (Zfyve28), mRNA mm
RAE230B:1398556_AT zinc finger, FYVE domain containing 28 (predicted) rn
RAT230_2:1398556_AT zinc finger, FYVE domain containing 28 (predicted) rn
RAE230A:1374240_AT Zinc finger, FYVE domain containing 28 (predicted) rn
RAT230_2:1374240_AT Zinc finger, FYVE domain containing 28 (predicted) rn
RAE230B:1381154_AT Zinc finger, FYVE domain containing 28 (predicted) rn
RAT230_2:1381154_AT Zinc finger, FYVE domain containing 28 (predicted) rn
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC68738 Lateral signaling target protein 2 [Caenorhabditis elegans] ref|NP_508756.2| Lateral Signaling Target family member (lst-2) [Caenorhabditis elegans] 0.0
blast CAE68590 Hypothetical protein CBG14460 [Caenorhabditis briggsae] 0.0
blast XP_700230 PREDICTED: similar to Zinc finger FYVE domain containing protein 28 [Danio rerio] 7.0E-79
Protein Domains
Database ID Description E-Value
Pfam IPR000306 EMBL-EBI Zn-finger, FYVE type 1.2E-23
Pfam IPR000306 EMBL-EBI Zn-finger, FYVE type 1.2E-23
Sequence
Target Sequence 
>C. ELEGANS:178082_S_AT
gatgcaaaccgatcccagatctcaaattgatccgaaaaacttgcgctcacgcttccgttc
atccgaagatcttgttcatcgactatttgtgtgcatcgccggtgttgccgatcagcttca
gaccaactattcgagcgaaattcgaaaagtgttgaagctgattcttcagccatcagaaat
cattccggtctacgaggtggtcaacgcgcaagtggcgaattctcagactgaaggagaaga
aactggcgttgaggcccaggaaacgctaccacttcccgcattcatgggtgtccgttgggt
tccggatgaagactgcgagcagtgcaccgcctgctcaatgccattcaactttgttaggcg
acgacaccactgtcgaaactgtggacgcattttttgccacaagtgctcgtgcaacacgat
cagcattccagaacacggatatgatagaaagcccgttgtgtcttccccccaatccaacct
ctcacttttaacacgtcaagaccaattttcttgtaatttgctccatcaatcccac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATGCAAACCGATCCCAGATCTCAA 696 411 1404 Antisense
TGATCCGAAAAACTTGCGCTCACGC 628 565 1431 Antisense
ACGCTTCCGTTCATCCGAAGATCTT 115 91 1452 Antisense
TCGACTATTTGTGTGCATCGCCGGT 421 601 1482 Antisense
GATCAGCTTCAGACCAACTATTCGA 462 421 1513 Antisense
ATCATTCCGGTCTACGAGGTGGTCA 521 29 1582 Antisense
TACCACTTCCCGCATTCATGGGTGT 387 649 1670 Antisense
TCATGGGTGTCCGTTGGGTTCCGGA 228 617 1685 Antisense
GCAACACGATCAGCATTCCAGAACA 476 319 1814 Antisense
CAACCTCTCACTTTTAACACGTCAA 515 185 1878 Antisense
TTGTAATTTGCTCCATCAATCCCAC 134 709 1914 Antisense
Sequence Source Affymetrix Proprietary Database