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[178074_at]

Description M04D8.7 /REP_DB=WormBase Gene ID /WP=CE00580 /TR=Q21512 /GB=CAA83615.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 55      6grp_high1_10_10 > 44      4grp_highest_8_8 > 39      4grp_high_8_8 > 12      6grp_highest_nonorm > 74      6grp_total > 65      4grp_total > 13      6grp_high1_sub > 02      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1716
1 2 3 4 range
A 1156.9
(-0.8)
P 		722.8
(-3)
P 		840.9
(-2.4)
P 		1887.3
(3)
P 		1165
B 1090.4
(-1.1)
P 		871.8
(-1.9)
P 		573.3
(-3)
P 		2228
(3)
P 		1655
C 1207.1
(1.3)
P 		701.5
(-1.8)
P 		512.4
(-3)
P 		1476
(3)
P 		964
avr 1151.5
(-0.5) 		765.4
(-2.4) 		642.2
(-3) 		1863.8
(3) 		1222

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 411
1 2 3 4 5 6 range
A 593.9
(-2.8)
P 		812.4
(1)
P 		928.1
(3)
P 		717.8
(-0.6)
P 		582.4
(-3)
P 		636.5
(-2.1)
P 		346
B 735
(1.8)
P 		789.6
(3)
P 		516.8
(-3)
P 		781.1
(2.8)
P 		753.7
(2.2)
P 		763.5
(2.4)
P 		273
C 603.7
(-3)
P 		673.5
(-1.5)
P 		687.8
(-1.2)
P 		746.8
(0)
P 		615.5
(-2.8)
P 		887.2
(3)
P 		284
avr 644.2
(-3) 		758.5
(2.8) 		710.9
(0.4) 		748.6
(2.3) 		650.5
(-2.7) 		762.4
(3) 		118
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 342
1 2 3 4 range
A 421.2
(3)
P 		156.9
(-3)
P 		219.8
(-1.6)
P 		276.3
(-0.3)
P 		264
B 347.7
(0.8)
P 		215.3
(-1.6)
P 		132.8
(-3)
P 		474.8
(3)
P 		342
C 399.6
(3)
P 		201.7
(-2.4)
P 		178.6
(-3)
P 		248.9
(-1.1)
P 		221
avr 389.5
(3) 		191.3
(-2.6) 		177.1
(-3) 		333.3
(1.4) 		212

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 197
1 2 3 4 5 6 range
A 109.5
(-3)
P 		288.9
(3)
P 		202.1
(0.1)
P 		182
(-0.6)
P 		175.8
(-0.8)
P 		155
(-1.5)
P 		179
B 255.8
(1.3)
P 		262.8
(1.6)
P 		125.5
(-3)
P 		206.9
(-0.3)
P 		306.2
(3)
P 		284.7
(2.3)
P 		181
C 223.8
(0)
P 		205.5
(-0.7)
P 		175.1
(-2)
P 		150.2
(-3)
P 		199.2
(-1)
P 		297
(3)
P 		147
avr 196.4
(-1) 		252.4
(3) 		167.6
(-3) 		179.7
(-2.1) 		227.1
(1.2) 		245.6
(2.5) 		85
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178074_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6476
Sequence Type Exemplar sequence
Representative Public ID M04D8.7 NCBI
Target Description M04D8.7 /REP_DB=WormBase Gene ID /WP=CE00580 /TR=Q21512 /GB=CAA83615.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066827(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066827 NCBI Caenorhabditis elegans M04D8.7 (M04D8.7) mRNA, complete cds. 11/11 None
GENEFINDER00000022951 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:10037344:10038102:1 11/11 None
M04D8.7 ENSEMBL cdna:known chromosome:CEL140:III:10037344:10038102:1 gene:M04D8.7 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000022947 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:10037339-10038098(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl M04D8.7
Entrez Gene 187446 Entrez gene
SwissProt Q21512 EMBL-EBI
WormBase CE00580 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA83615 Hypothetical protein M04D8.7 [Caenorhabditis elegans] ref|NP_499228.1| M04D8.7 [Caenorhabditis elegans] 1.0E-94
blast CAE65060 Hypothetical protein CBG09906 [Caenorhabditis briggsae] 6.0E-53
blast CAD90169 Hypothetical protein C05B5.8 [Caenorhabditis elegans] ref|NP_001021142.1| C05B5.8 [Caenorhabditis elegans] sp|Q86DA7|YKO8_CAEEL Hypothetical protein C05B5.8 in chromosome III 9.0E-23
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_499228.1 3 33-55,68-90,119-141
M04D8.7 3 33-55,68-90,119-141
GENEFINDER00000022951 3 33-55,68-90,119-141
Sequence
Target Sequence 
>C. ELEGANS:178074_AT
tcgtccagtgatttatgctctcgcatcggccggaatccttcgttcaggagctcaatttta
ctatggccctcacggcattttcctgtctctgattccaattgcctacctattcttcaatgg
tttcttgattttcgccgttgccaagcgagacgttaaacacctcaaatgggctcaaagact
cactatgactgcaacaattctatcagtgatcccatttctactgttcccagtcgtgtctgc
cagcttctttgcatcgggagaaatagaagcaatagaaaagaacggaactcattttcgtcc
tgaacattacggaaatatgacttccccagactttcgctttgttttcggggtcgtcgccgg
gttctgtgttgaaattggagccgcgttctttattgcggttgagctcttcaagtacattct
tgtgagcagaatctggctatccgaagtcaactggaccct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCGTCCAGTGATTTATGCTCTCGCA 535 605 36 Antisense
AGCTCAATTTTACTATGGCCCTCAC 531 79 84 Antisense
TTCCAATTGCCTACCTATTCTTCAA 594 701 128 Antisense
CAATGGTTTCTTGATTTTCGCCGTT 691 181 150 Antisense
TTTCGCCGTTGCCAAGCGAGACGTT 621 667 165 Antisense
TCAGTGATCCCATTTCTACTGTTCC 476 623 238 Antisense
GAACTCATTTTCGTCCTGAACATTA 362 349 320 Antisense
GAAATATGACTTCCCCAGACTTTCG 22 363 347 Antisense
GTCGCCGGGTTCTGTGTTGAAATTG 66 239 388 Antisense
GGAGCCGCGTTCTTTATTGCGGTTG 635 521 412 Antisense
GGCTATCCGAAGTCAACTGGACCCT 17 539 470 Antisense
Sequence Source Affymetrix Proprietary Database