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Cluster Members
Consensus/Exemplar

GeneChip Array Information

Probe Set ID

178064_at

GeneChip Array

C. elegans Genome Array

Organism Common Name

Nematode

Probe Design Information

Transcript ID(Array Design)

affy.Ce.1276

Sequence Type

Exemplar sequence

Representative Public ID

T23D8.3 NCBI

Target Description

T23D8.3 /REP_DB=WormBase Gene ID /WP=CE14052 /TR=O02327 /GB=CAB03401.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation

Annotation Method Description

Annotation Description

This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).

Annotation Grade

This is a grade A annotation.

Annotation Transcript Cluster (# of Matching Probes)

NM_060236(11)

Transcript Assignments

Representative Transcript	Description	Matching Probes	Related Probesets by Grade
NM_060236 NCBI	Caenorhabditis elegans T23D8.3 (T23D8.3) mRNA, complete cds.	11/11	None
SNAP00000036748 ENSEMBL	cdna:SNAP chromosome:CEL140:I:9981699:9984407:-1	10/11	None
GENEFINDER00000036759 ENSEMBL	cdna:GeneFinder chromosome:CEL140:I:9981699:9984407:-1	11/11	None
T23D8.3 ENSEMBL	cdna:known chromosome:CEL140:I:9981089:9984436:-1 gene:T23D8.3	11/11	None

Annotation Notes

There are no noteworthy cross hybridizing mRNAs found for this probe set.

Genomic Alignment of Consensus/Exemplar Sequence

Assembly

Wormbase March 2004

Alignment(s)

Position	Identity	Coverage	Cytoband
chrI:9981629-9984338(-)	91.4	100.0

Public Domain and Genome References

Gene Title

Ensembl

T23D8.3

Entrez Gene

172857 Entrez gene

SwissProt

O02327 EMBL-EBI

WormBase

CE14052 Wormbase

Functional Annotations

Ortholog

ID	Title	Organism
ATH1-121501:252239_AT	expressed protein	at
CANINE_2:CFA.2235.1.S1_AT	similar to CG7686-PA	cfa
CANINE_2:CFAAFFX.1417.1.S1_S_AT	similar to CG7686-PA	cfa
DROSOPHILA_2:1638367_AT		dm
DROSGENOME1:154018_AT		dm
CHICKEN:GGA.14413.1.S1_AT	similar to RIKEN cDNA 2610020N02 gene	gga
HU35KSUBC:RC_AA453435_AT	chromosome 6 open reading frame 93	hs
U133_X3P:HS.61164.0.A1_3P_S_AT	chromosome 6 open reading frame 93	hs
U133_X3P:HS.61164.0.A1_3P_AT	chromosome 6 open reading frame 93	hs
U133_X3P:HS.33944.0.A1_3P_AT	chromosome 6 open reading frame 93	hs
U133_X3P:225748_3P_AT	chromosome 6 open reading frame 93	hs
HG-U133_PLUS_2:225748_AT	chromosome 6 open reading frame 93	hs
HG-U133B:225748_AT	chromosome 6 open reading frame 93	hs
HG-U133_PLUS_2:239212_AT	chromosome 6 open reading frame 93	hs
HG-U133B:239212_AT	chromosome 6 open reading frame 93	hs
HG-U95B:46171_AT	chromosome 6 open reading frame 93	hs
HG-U95B:50925_R_AT	Chromosome 6 open reading frame 93	hs
HU35KSUBB:RC_W86002_AT	Chromosome 6 open reading frame 93	hs
MU11KSUBA:U01139_S_AT	RIKEN cDNA 2610020N02 gene	mm
MG-U74AV2:160521_AT	RIKEN cDNA 2610020N02 gene	mm
MOE430A:1426813_AT	RIKEN cDNA 2610020N02 gene	mm
MOUSE430A_2:1426813_AT	RIKEN cDNA 2610020N02 gene	mm
MOUSE430_2:1426813_AT	RIKEN cDNA 2610020N02 gene	mm
MU19KSUBB:TC27145_AT	RIKEN cDNA 2610020N02 gene, mRNA (cDNA clone MGC:78294 IMAGE:5038459)	mm
MU19KSUBB:TC27145_G_AT	RIKEN cDNA 2610020N02 gene, mRNA (cDNA clone MGC:78294 IMAGE:5038459)	mm
RG-U34C:H34870_AT	similar to hypothetical protein FLJ14909 (predicted)	rn
RG-U34C:RC_AI071749_AT	similar to hypothetical protein FLJ14909 (predicted)	rn
RG-U34C:RC_AI176212_AT	similar to hypothetical protein FLJ14909 (predicted)	rn
RAE230A:1372312_AT	similar to hypothetical protein FLJ14909 (predicted)	rn
RAT230_2:1372312_AT	similar to hypothetical protein FLJ14909 (predicted)	rn
YEAST_2:1774933_AT	Protein required for growth at low temperature	Sc

Protein Similarities

Method	ID	Description	E-Value
blast	CAB03401	Hypothetical protein T23D8.3 [Caenorhabditis elegans] ref\|NP_492637.1\| T23D8.3 [Caenorhabditis elegans]	0.0
blast	CAE60248	Hypothetical protein CBG03822 [Caenorhabditis briggsae]	1.0E-173
blast	CAE60248	Hypothetical protein CBG03822 [Caenorhabditis briggsae]	1.0E-172
blast	XP_393157	PREDICTED: similar to ENSANGP00000016262 [Apis mellifera]	2.0E-29

Protein Domains

Database	ID	Description	E-Value
Pfam	IPR007307 EMBL-EBI	Low temperature viability protein	1.9E-6
Pfam	IPR007307 EMBL-EBI	Low temperature viability protein	6.5E-8
Pfam	IPR007307 EMBL-EBI	Low temperature viability protein	4.4E-7
Pfam	IPR007307 EMBL-EBI	Low temperature viability protein	6.5E-8
Pfam	IPR007307 EMBL-EBI	Low temperature viability protein	4.4E-7

Sequence

Target Sequence

>C. ELEGANS:178064_AT
cgtgaatacaacaccgatcagatcggagagcttgatggtgacgactatgatgttggagga
gcacttgaaccgaacgctggacgacttcacaaacttataaatgacaaaggaccttcaaat
gcagagtatgatgaagagctcgccaagcattatgtacgtgaacgaatgcgtctcattgaa
gagggagtcatcaaagacaaagaagaatacgagattgtggaagtggatgagggtacaaat
aagaaaatgaaatgggattgtgaatcatttgctactcaatacactaatatctacaatcat
ccgacactcatcaaggagccacgtggtttatccagaaaggcattgaaacgattcgataaa
gctgttgaagagatggatattgctgaagaagatgaagatgatgatgaggatatggaggat
gatgatgataaggaaagcgttttttcaactgtttctactttccgaccaaaaaatgaaact
ccggaacagagaagtctgcggaaaaaagctgtaaaagaggccagaaagctgagaagagtt
gagaaaaaggctaacaagactatgttcgccgag

BLASTn GenBank NR

Probe Info

Probe Sequence(5'-3')	Probe X	Probe Y	Probe Interrogation Position	Strandedness
CGTGAATACAACACCGATCAGATCG	530	243	616	Antisense
GGAGAGCTTGATGGTGACGACTATG	126	517	640	Antisense
GGAGCACTTGAACCGAACGCTGGAC	87	521	673	Antisense
AACGCTGGACGACTTCACAAACTTA	70	147	688	Antisense
GAGCTCGCCAAGCATTATGTACGTG	614	387	751	Antisense
GTACGTGAACGAATGCGTCTCATTG	455	459	769	Antisense
GAATCATTTGCTACTCAATACACTA	541	323	877	Antisense
TATCTACAATCATCCGACACTCATC	227	659	903	Antisense
GACACTCATCAAGGAGCCACGTGGT	527	367	918	Antisense
GGAGCCACGTGGTTTATCCAGAAAG	643	521	930	Antisense
GGCTAACAAGACTATGTTCGCCGAG	126	539	1164	Antisense

Sequence Source

Affymetrix Proprietary Database

Description	T23D8.3 /REP_DB=WormBase Gene ID /WP=CE14052 /TR=O02327 /GB=CAB03401.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation
Cluster	6grp_highest_10_10 > 83 6grp_high1_10_10 > 47 4grp_highest_8_8 > 30 4grp_high_8_8 > 05 6grp_highest_nonorm > 86 6grp_total > 37 4grp_total > 03 6grp_highest_sub > Cu 6grp_high1_sub > Cu 6grp_total_sub > Cup 4grp_rule_based > L4