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[178061_at]

Description F25D1.4 /REP_DB=WormBase Gene ID /WP=CE05725 /TR=Q19777 /GB=CAA98267.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 24      4grp_total > 30      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 74
1 2 3 4 range
A 58.5
(3)
P 		23.4
(-1)
A 		6.4
(-3)
A 		35.1
(0.3)
A 		52
B 36.2
(0.5)
P 		9.4
(-3)
A 		32.8
(0.1)
A 		55.2
(3)
A 		46
C 78.6
(3)
P 		44.3
(0.2)
A 		4.5
(-3)
A 		23.4
(-1.5)
A 		74
avr 57.8
(3) 		25.7
(-1.5) 		14.6
(-3) 		37.9
(0.2) 		43

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 73
1 2 3 4 5 6 range
A 66.8
(3)
A 		39
(0.3)
A 		44.7
(0.9)
A 		10.3
(-2.4)
A 		27.7
(-0.7)
A 		3.9
(-3)
A 		63
B 75
(3)
P 		47.7
(0.7)
A 		55.7
(1.4)
A 		9
(-2.5)
A 		2.8
(-3)
A 		12.4
(-2.2)
A 		72
C 62.3
(2.6)
P 		66.4
(3)
P 		39
(0.4)
A 		16.3
(-1.7)
A 		2.8
(-2.9)
A 		2
(-3)
A 		64
avr 68
(3) 		51
(1.4) 		46.5
(0.9) 		11.9
(-2.4) 		11.1
(-2.5) 		6.1
(-3) 		62
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 24
1 2 3 4 range
A 21.3
(3)
P 		5.1
(-2)
A 		1.7
(-3)
A 		5.1
(-2)
A 		20
B 11.5
(2.8)
P 		2.3
(-3)
A 		7.6
(0.3)
A 		11.8
(3)
A 		10
C 26
(3)
P 		12.7
(-0.3)
A 		1.6
(-3)
A 		3.9
(-2.4)
A 		24
avr 19.6
(3) 		6.7
(-1.8) 		3.6
(-3) 		6.9
(-1.8) 		16

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 25
1 2 3 4 5 6 range
A 12.3
(2.3)
A 		13.9
(3)
A 		9.7
(1.1)
A 		2.6
(-2.2)
A 		8.4
(0.5)
A 		0.9
(-3)
A 		13
B 26.1
(3)
P 		15.9
(0.6)
A 		13.5
(-0)
A 		2.4
(-2.7)
A 		1.1
(-3)
A 		4.6
(-2.2)
A 		25
C 23.1
(3)
P 		20.3
(2.3)
P 		9.9
(-0.5)
A 		3.3
(-2.3)
A 		0.9
(-2.9)
A 		0.7
(-3)
A 		22
avr 20.5
(3) 		16.7
(1.8) 		11
(-0.1) 		2.8
(-2.8) 		3.5
(-2.5) 		2.1
(-3) 		18
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178061_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11414
Sequence Type Exemplar sequence
Representative Public ID F25D1.4 NCBI
Target Description F25D1.4 /REP_DB=WormBase Gene ID /WP=CE05725 /TR=Q19777 /GB=CAA98267.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073302(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073302 NCBI Caenorhabditis elegans F25D1.4 (F25D1.4) mRNA, complete cds. 11/11 None
SNAP00000015980 ENSEMBL cdna:SNAP chromosome:CEL140:V:10545398:10547918:1 9/11 None
GENEFINDER00000015986 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:10542962:10548323:1 10/11 None
F25D1.4 ENSEMBL cdna:known chromosome:CEL140:V:10542962:10547925:1 gene:F25D1.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:10542961-10547925(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F25D1.4
Entrez Gene 184921 Entrez gene
SwissProt Q19777 EMBL-EBI
WormBase CE05725 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6814 sodium ion transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5272 sodium channel activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA98267 Hypothetical protein F25D1.4 [Caenorhabditis elegans] ref|NP_505703.1| F25D1.4 [Caenorhabditis elegans] 0.0
blast CAE64838 Hypothetical protein CBG09634 [Caenorhabditis briggsae] 0.0
blast AAH95568 Amiloride-sensitive cation channel 4 b [Danio rerio] 2.0E-13
Protein Domains
Database ID Description E-Value
Pfam IPR001873 EMBL-EBI Na+ channel, amiloride-sensitive 5.4E-106
Pfam IPR001873 EMBL-EBI Na+ channel, amiloride-sensitive 3.7E-70
Pfam IPR001873 EMBL-EBI Na+ channel, amiloride-sensitive 6.9E-4
Pfam IPR001873 EMBL-EBI Na+ channel, amiloride-sensitive 2.7E-93
Pfam IPR001873 EMBL-EBI Na+ channel, amiloride-sensitive 2.5E-6
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_505703.1 1 62-84
F25D1.4 1 62-84
GENEFINDER00000015986 2 131-153,904-926
Sequence
Target Sequence 
>C. ELEGANS:178061_AT
ttttcaagaatcgctacactccacttattgaagcatctaacgtccatacaaaaacaaatc
caaattcggaatcatttaaactttacacagaagccatgaagaaagtgttggctaaactgc
aaatattaaaaacaagagttcgaatgagtgaatggaaagattttaaaattgatttaaaag
aatttgaatcatcgtatcgagaaatagcaaaagaccatgtggaaattgaagaaatgctaa
aattaagaaaaactatggttacggatattccagcattggcgaatgaattatcagaaacct
ttttggcagcaacagaaagtagaagaaagtttgcagtattgacaggaattgctgcagatg
aaagtttcagtgataaattttcaaatttctcgaaatgtttggatgaacttcacgaaaaag
cgccaaatttaaaggcaagaataattactgactttgaaatattcaaaatacctaaaaatt
acatttatttatctgattttaatttctatattttaaagaaaactcgattcatccgtggcg
aatggctttcaagacttcgaaaccaagttttgatggcccagtc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTTTCAAGAATCGCTACACTCCACT 6 677 1922 Antisense
TACACTCCACTTATTGAAGCATCTA 674 641 1936 Antisense
GAAGCATCTAACGTCCATACAAAAA 610 341 1951 Antisense
GAAAGTGTTGGCTAAACTGCAAATA 560 359 2022 Antisense
GGTTACGGATATTCCAGCATTGGCG 264 507 2178 Antisense
ATCAGAAACCTTTTTGGCAGCAACA 396 27 2211 Antisense
TGGATGAACTTCACGAAAAAGCGCC 106 549 2321 Antisense
AAGAAAACTCGATTCATCCGTGGCG 405 153 2437 Antisense
TCATCCGTGGCGAATGGCTTTCAAG 524 621 2450 Antisense
ATGGCTTTCAAGACTTCGAAACCAA 64 53 2463 Antisense
GAAACCAAGTTTTGATGGCCCAGTC 576 357 2480 Antisense
Sequence Source Affymetrix Proprietary Database