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[178056_at]

Description F25H8.2 /REP_DB=WormBase Gene ID /WP=CE05728 /TR=Q19788 /GB=CAA93283.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 97      6grp_high1_10_10 > 78      4grp_highest_8_8 > 45      4grp_high_8_8 > 08      6grp_highest_nonorm > 92      6grp_total > 88      4grp_total > 15      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 220
1 2 3 4 range
A 422.5
(3)
P 		236.6
(-3)
P 		303.7
(-0.8)
P 		393.6
(2.1)
P 		186
B 451
(3)
P 		235.1
(-3)
P 		240
(-2.9)
P 		419.8
(2.1)
P 		216
C 454.9
(3)
P 		240.8
(-3)
P 		360.1
(0.3)
P 		357.6
(0.3)
P 		214
avr 442.8
(3) 		237.5
(-3) 		301.3
(-1.1) 		390.3
(1.5) 		205

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 351
1 2 3 4 5 6 range
A 319.9
(-1.3)
P 		252.4
(-3)
P 		277.7
(-2.3)
P 		307.3
(-1.6)
P 		436.3
(1.7)
P 		485.9
(3)
P 		234
B 345.3
(-1.2)
P 		362.1
(-0.9)
P 		238.5
(-3)
P 		290.7
(-2.1)
P 		338.9
(-1.3)
P 		589.2
(3)
P 		351
C 293.6
(-2.1)
P 		340.8
(-0.8)
P 		259.8
(-3)
P 		373.5
(0)
P 		433
(1.6)
P 		485.6
(3)
P 		226
avr 319.6
(-1.6) 		318.4
(-1.6) 		258.7
(-3) 		323.8
(-1.5) 		402.7
(0.3) 		520.2
(3) 		262
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 102
1 2 3 4 range
A 153.8
(3)
P 		51.4
(-3)
P 		79.4
(-1.4)
P 		57.6
(-2.6)
P 		102
B 143.8
(3)
P 		58.1
(-2.8)
P 		55.6
(-3)
P 		89.5
(-0.7)
P 		88
C 150.6
(3)
P 		69.2
(-2.4)
P 		125.5
(1.3)
P 		60.3
(-3)
P 		90
avr 149.4
(3) 		59.6
(-3) 		86.8
(-1.2) 		69.1
(-2.4) 		90

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 162
1 2 3 4 5 6 range
A 59
(-3)
P 		89.8
(-0.5)
P 		60.5
(-2.9)
P 		77.9
(-1.4)
P 		131.7
(3)
P 		118.3
(1.9)
P 		73
B 120.2
(-0.7)
P 		120.5
(-0.7)
P 		57.9
(-3)
P 		77
(-2.3)
P 		137.7
(-0)
P 		219.7
(3)
P 		162
C 108.8
(-0.3)
P 		104
(-0.6)
P 		66.1
(-3)
P 		75.1
(-2.4)
P 		140.1
(1.6)
P 		162.6
(3)
P 		97
avr 96
(-1) 		104.8
(-0.5) 		61.5
(-3) 		76.7
(-2.1) 		136.5
(1.3) 		166.9
(3) 		105
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178056_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9738
Sequence Type Exemplar sequence
Representative Public ID F25H8.2 NCBI
Target Description F25H8.2 /REP_DB=WormBase Gene ID /WP=CE05728 /TR=Q19788 /GB=CAA93283.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069389(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069389 NCBI Caenorhabditis elegans F25H8.2 (F25H8.2) mRNA, complete cds. 11/11 None
SNAP00000013891 ENSEMBL cdna:SNAP chromosome:CEL140:IV:9931543:9933736:1 11/11 None
GENEFINDER00000013897 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:9932325:9933736:1 11/11 None
F25H8.2.2 ENSEMBL cdna:known chromosome:CEL140:IV:9932255:9933859:1 gene:F25H8.2 11/11 None
F25H8.2.1 ENSEMBL cdna:known chromosome:CEL140:IV:9932255:9933793:1 gene:F25H8.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:9932330-9933742(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F25H8.2
Entrez Gene 177848 Entrez gene
SwissProt Q19788 EMBL-EBI
WormBase CE05728 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA93283 Hypothetical protein F25H8.2 [Caenorhabditis elegans] ref|NP_501790.1| F25H8.2 [Caenorhabditis elegans] 0.0
blast CAE59964 Hypothetical protein CBG03454 [Caenorhabditis briggsae] 1.0E-128
Protein Domains
Database ID Description E-Value
Pfam IPR008696 EMBL-EBI NAF1 4.6E-11
Pfam IPR008696 EMBL-EBI NAF1 4.6E-11
Sequence
Target Sequence 
>C. ELEGANS:178056_AT
tctcgacttcgattcatttcttttcgatcaaaaaggaaatgcgttgggacaaatttatga
catttttggacaagtcaaaaatccacaatacgttattcgattcaattcttgtgaagaggc
atcgatgatacccattgacatgaaagtatattatgctcctacagaagaacaattttccaa
aactccattcaaaggacttaatcttgctgcagcaaatagggatgctattaaatccctaaa
tcgtcgcattgatcatcaagaagctgttgcaaaagctggtgaaacgatggctcaggttgg
catcggtagtgacgtggagttcagtgatgatgaagcggagaaagaatttaaacgcaccac
atatacccaaccagctagtcaaatgtcaagaggattcgattcgaacagagggcgaaaacg
agatcgccgcggaggacaaaaagttagattttctgctcagaatccacctgccagatcgga
taatcttgggcccatccctcagcagccatctgcgccaccgccacctgctcgtccagcaca
atcaaccgaatc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCTCGACTTCGATTCATTTCTTTTC 429 611 576 Antisense
GAGGCATCGATGATACCCATTGACA 426 405 691 Antisense
AAGGACTTAATCTTGCTGCAGCAAA 584 159 767 Antisense
TTAAATCCCTAAATCGTCGCATTGA 165 683 803 Antisense
GGCTCAGGTTGGCATCGGTAGTGAC 547 523 864 Antisense
TTTAAACGCACCACATATACCCAAC 97 667 922 Antisense
ATACCCAACCAGCTAGTCAAATGTC 263 23 938 Antisense
GAAAACGAGATCGCCGCGGAGGACA 159 353 989 Antisense
TCAGAATCCACCTGCCAGATCGGAT 174 621 1032 Antisense
AGATCGGATAATCTTGGGCCCATCC 267 67 1048 Antisense
CTCGTCCAGCACAATCAACCGAATC 210 233 1103 Antisense
Sequence Source Affymetrix Proprietary Database