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[178049_at]

Description T27A8.4 /REP_DB=WormBase Gene ID /WP=CE03744 /TR=Q22828 /GB=CAA92225.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Cluster 6grp_total > 70      4grp_total > 17      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 41
1 2 3 4 range
A 3
(-3)
A 		5.1
(-2.7)
A 		6.7
(-2.5)
A 		44.1
(3)
A 		41
B 3.8
(1.5)
A 		2.9
(-3)
A 		4.1
(3)
A 		4.1
(3)
A 		1
C 3.4
(-3)
A 		13.9
(3)
A 		5.1
(-2)
A 		5.9
(-1.6)
A 		11
avr 3.4
(-3) 		7.3
(-1.4) 		5.3
(-2.2) 		18
(3) 		15

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 39
1 2 3 4 5 6 range
A 10
(-1.7)
A 		1.6
(-3)
A 		4.7
(-2.5)
A 		39.6
(3)
A 		4.1
(-2.6)
A 		1.8
(-3)
A 		38
B 3.4
(-2.2)
A 		19.4
(3)
A 		7.8
(-0.8)
A 		2.8
(-2.4)
A 		3.1
(-2.3)
A 		0.9
(-3)
A 		19
C 1.2
(-3)
A 		2.2
(-2.3)
A 		10
(3)
A 		2.5
(-2.1)
A 		1.3
(-2.9)
A 		1.9
(-2.5)
A 		9
avr 4.9
(-1.5) 		7.7
(-0.2) 		7.5
(-0.3) 		15
(3) 		2.8
(-2.4) 		1.5
(-3) 		14
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 6
1 2 3 4 range
A 1.1
(-3)
A 		1.1
(-3)
A 		1.7
(-2.3)
A 		6.5
(3)
A 		5
B 1.2
(3)
A 		0.7
(-3)
A 		1
(0.6)
A 		0.9
(-0.6)
A 		1
C 1.1
(-2.8)
A 		4
(3)
A 		1.8
(-1.4)
A 		1
(-3)
A 		3
avr 1.1
(-3) 		1.9
(-0.2) 		1.5
(-1.6) 		2.8
(3) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 10
1 2 3 4 5 6 range
A 1.9
(-2.1)
A 		0.6
(-2.9)
A 		1
(-2.6)
A 		10
(3)
A 		1.2
(-2.5)
A 		0.4
(-3)
A 		10
B 1.2
(-2.1)
A 		6.5
(3)
A 		1.9
(-1.5)
A 		0.7
(-2.6)
A 		1.3
(-2)
A 		0.3
(-3)
A 		6
C 0.4
(-3)
A 		0.7
(-2.1)
A 		2.5
(3)
A 		0.5
(-2.7)
A 		0.4
(-3)
A 		0.6
(-2.4)
A 		2
avr 1.2
(-1.5) 		2.6
(1) 		1.8
(-0.5) 		3.7
(3) 		1
(-1.9) 		0.4
(-3) 		3
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 178049_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17991
Sequence Type Exemplar sequence
Representative Public ID T27A8.4 NCBI
Target Description T27A8.4 /REP_DB=WormBase Gene ID /WP=CE03744 /TR=Q22828 /GB=CAA92225.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_078228(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_078228 NCBI Caenorhabditis elegans T27A8.4 (T27A8.4) mRNA, complete cds. 11/11 None
SNAP00000006726 ENSEMBL cdna:SNAP chromosome:CEL140:X:16064466:16067377:-1 10/11 None
T27A8.4 ENSEMBL cdna:known chromosome:CEL140:X:16066983:16067377:-1 gene:T27A8.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000006727 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:16066982-16067377(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T27A8.4
Entrez Gene 188969 Entrez gene
SwissProt Q22828 EMBL-EBI
WormBase CE03744 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA92224 Hypothetical protein T27A8.5 [Caenorhabditis elegans] ref|NP_510628.1| T27A8.5 [Caenorhabditis elegans] 1.0E-129
blast NP_508311 C36C9.4 [Caenorhabditis elegans] gb|AAC67426.1| Hypothetical protein C36C9.4 [Caenorhabditis elegans] 2.0E-71
blast CAE70402 Hypothetical protein CBG16972 [Caenorhabditis briggsae] 3.0E-57
blast CAA92225 Hypothetical protein T27A8.4 [Caenorhabditis elegans] ref|NP_510629.1| T27A8.4 [Caenorhabditis elegans] 1.0E-52
Protein Domains
Database ID Description E-Value
Pfam IPR009667 EMBL-EBI Protein of unknown function DUF1258 2.7E-100
Sequence
Target Sequence 
>C. ELEGANS:178049_AT
atgcctcgatcaattgcgaagctgttcgatcccattcggtggcaacaggagccagttgtt
gataatgaagaggttgtgcctggagagaggacatctggagagaatcaagatctaggcaga
gaagagaatggaagtgaagaggatgagatccaacaagttgagacgcaaagcgacgacgga
aattactctcctccctctggaacccctgaaaccgaagtagaaacacctgcatcacagccg
caagatgttgaggatgac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGCCTCGATCAATTGCGAAGCTGT 610 41 13 Antisense
ATTGCGAAGCTGTTCGATCCCATTC 449 7 25 Antisense
GATCCCATTCGGTGGCAACAGGAGC 179 417 40 Antisense
CAGGAGCCAGTTGTTGATAATGAAG 680 207 58 Antisense
GAGGTTGTGCCTGGAGAGAGGACAT 171 403 82 Antisense
GAGAGGACATCTGGAGAGAATCAAG 323 395 97 Antisense
GATGAGATCCAACAAGTTGAGACGC 294 411 154 Antisense
GTTGAGACGCAAAGCGACGACGGAA 652 435 169 Antisense
GAAGTAGAAACACCTGCATCACAGC 251 337 226 Antisense
ACCTGCATCACAGCCGCAAGATGTT 698 85 237 Antisense
ACAGCCGCAAGATGTTGAGGATGAC 517 107 246 Antisense
Sequence Source Affymetrix Proprietary Database